(Circular) (Six-base) MAPSORT of: pFN21AB4976.seq Check: 5271 from: 1 to: 5959 pFN21AB4976 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2064 2817 3302 1937 Size: 127 753 485 4594 Fragments arranged by size: 4594 753 485 127 Acc65I G'GTAC_C Cuts at: 3230 3280 3230 Size: 50 5909 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2743 1184 Size: 1559 4400 AlwNI CAG_nnn'CTG Cuts at: 3190 3702 5274 3190 Size: 512 1572 3875 Fragments arranged by size: 3875 1572 512 ApaI G_GGCC'C Cuts at: 2403 2403 Size: 5959 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2461 2488 3268 3494 3646 5755 1493 Size: 968 27 780 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 780 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2276 2994 3284 1870 Size: 73 79 254 718 290 4545 Fragments arranged by size: 4545 718 290 254 79 73 BamHI G'GATC_C Cuts at: 2134 3289 3656 2134 Size: 1155 367 4437 Fragments arranged by size: 4437 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 2930 3230 3280 4735 Size: 325 217 627 1004 139 300 50 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 1004 627 325 300 217 139 50 BanII G_rGCy'C Cuts at: 1391 2403 3278 1391 Size: 1012 875 4072 Fragments arranged by size: 4072 1012 875 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2412 2898 928 Size: 971 513 486 3989 Fragments arranged by size: 3989 971 513 486 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2781 2815 4150 4184 1903 Size: 34 844 34 1335 34 3678 Fragments arranged by size: 3678 1335 844 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2622 2700 3013 5954 2622 Size: 78 313 2941 2627 Fragments arranged by size: 2941 2627 313 78 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2403 2759 4020 5176 1790 Size: 613 356 1261 1156 2573 Fragments arranged by size: 2573 1261 1156 613 356 BmgBI CAC'GTC Cuts at: 1318 2662 3031 1318 Size: 1344 369 4246 Fragments arranged by size: 4246 1344 369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4619 1239 Size: 3380 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 4084 4949 5247 2867 Size: 1217 865 298 3579 Fragments arranged by size: 3579 1217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2552 4622 882 Size: 617 1053 2070 2219 Fragments arranged by size: 2219 2070 1053 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 2699 3126 5953 2699 Size: 427 2827 2705 Fragments arranged by size: 2827 2705 427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3113 3766 4148 4783 Size: 53 83 186 936 1580 653 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1580 1451 936 653 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2508 4392 4790 5064 5211 5622 2508 Size: 1884 398 274 147 411 2845 Fragments arranged by size: 2845 1884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3223 3253 1178 Size: 30 165 30 1820 30 3884 Fragments arranged by size: 3884 1820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 3236 1186 Size: 1297 753 3909 Fragments arranged by size: 3909 1297 753 BseYI C'CCAG_C Cuts at: 2326 2544 2795 5162 5658 2326 Size: 218 251 2367 496 2627 Fragments arranged by size: 2627 2367 496 251 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 4020 4105 5176 2337 Size: 1683 85 1071 3120 Fragments arranged by size: 3120 1683 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 3104 3104 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2403 2759 3278 4020 4105 5176 Size: 399 547 66 356 519 742 85 1071 Cuts at: 5176 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 547 519 399 356 85 66 BspEI T'CCGG_A Cuts at: 2508 5622 2508 Size: 3114 2845 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2186 3314 844 Size: 1342 1128 3489 Fragments arranged by size: 3489 1342 1128 BsrBI CCG'CTC Cuts at: 1394 1823 2155 3846 1394 Size: 429 332 1691 3507 Fragments arranged by size: 3507 1691 429 332 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2269 3233 4603 4790 1700 Size: 110 459 964 1370 187 2869 Fragments arranged by size: 2869 1370 964 459 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2102 4019 5031 2102 Size: 1917 1012 3030 Fragments arranged by size: 3030 1917 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 3031 3031 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2134 2358 2475 2622 2700 2943 3013 3289 Size: 162 224 117 147 78 243 70 276 Cuts at: 3289 3656 4041 4058 5510 5596 5608 5954 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 276 243 224 162 147 117 86 78 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2632 2766 3036 513 Size: 552 939 628 134 270 3436 Fragments arranged by size: 3436 939 628 552 270 134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2387 3475 4269 4289 2387 Size: 1088 794 20 4057 Fragments arranged by size: 4057 1088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2210 2438 4966 809 Size: 507 894 228 2528 1802 Fragments arranged by size: 2528 1802 894 507 228 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2247 3033 Size: 54 1425 50 275 18 364 53 786 Cuts at: 3033 3406 4295 5924 8 Size: 373 889 1629 43 Fragments arranged by size: 1629 1425 889 786 373 364 275 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 3213 3889 1353 Size: 1413 447 676 3423 Fragments arranged by size: 3423 1413 676 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4022 4619 5406 5674 1239 Size: 393 2390 597 787 268 1524 Fragments arranged by size: 2390 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2019 2203 2236 2399 2860 2019 Size: 184 33 163 461 5118 Fragments arranged by size: 5118 461 184 163 33 EcoRI G'AATT_C Cuts at: 2488 3268 3646 2488 Size: 780 378 4801 Fragments arranged by size: 4801 780 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2628 2660 3005 3037 4600 Size: 32 135 32 1056 32 345 32 1563 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1563 1056 345 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2653 3303 3549 669 Size: 988 281 715 650 246 3079 Fragments arranged by size: 3079 988 715 650 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3234 3284 3234 Size: 50 5909 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 5073 5257 1305 Size: 1257 2511 184 2007 Fragments arranged by size: 2511 2007 1257 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 3720 Fragments arranged by size: 3720 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3056 3917 4276 4435 518 Size: 648 1890 861 359 159 2042 Fragments arranged by size: 2042 1890 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3375 4052 5200 5445 1606 Size: 1769 677 1148 245 2120 Fragments arranged by size: 2120 1769 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2632 2766 513 Size: 552 939 628 134 3706 Fragments arranged by size: 3706 939 628 552 134 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2891 2923 4028 4060 5541 5573 2891 Size: 32 1105 32 1481 32 3277 Fragments arranged by size: 3277 1481 1105 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2019 2203 2236 2019 Size: 184 33 5742 Fragments arranged by size: 5742 184 33 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2399 2399 Size: 5959 PstI C_TGCA'G Cuts at: 830 2307 2394 3311 830 Size: 1477 87 917 3478 Fragments arranged by size: 3478 1477 917 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SanDI GG'GwC_CC Cuts at: 2019 2019 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2303 2390 3307 4442 5123 5314 826 Size: 219 1258 87 917 1135 681 191 1471 Fragments arranged by size: 1471 1258 1135 917 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2882 4063 4964 5226 820 Size: 197 926 939 1181 901 262 1553 Fragments arranged by size: 1553 1181 939 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2536 1517 Size: 1019 4940 StyI C'CwwG_G Cuts at: 513 1065 2004 2632 2766 2868 3398 513 Size: 552 939 628 134 102 530 3074 Fragments arranged by size: 3074 939 628 552 530 134 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2809 3986 4003 4156 4341 4786 325 Size: 1255 1229 1177 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1229 1177 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 4205 5917 Size: 274 80 33 51 1443 771 1457 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1457 1443 771 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI