(Circular) (Six-base) MAPSORT of: pFN21AB4948.seq Check: 6390 from: 1 to: 6445 pFN21AB4948 6445 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2116 2968 2116 Size: 852 5593 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2026 4255 278 Size: 53 83 186 936 490 2229 2468 Fragments arranged by size: 2468 2229 936 490 186 83 53 AccI GT'mk_AC Cuts at: 1937 2598 3788 1937 Size: 661 1190 4594 Fragments arranged by size: 4594 1190 661 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4508 5892 6160 1632 Size: 2876 1384 268 1917 Fragments arranged by size: 2876 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 2819 1166 Size: 1653 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AlwNI CAG_nnn'CTG Cuts at: 2146 2963 3409 3628 4188 5760 2146 Size: 817 446 219 560 1572 2831 Fragments arranged by size: 2831 1572 817 560 446 219 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 2008 2378 3754 3980 4132 6241 1493 Size: 515 370 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 515 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 3468 3770 1870 Size: 73 1525 302 4545 Fragments arranged by size: 4545 1525 302 73 BamHI G'GATC_C Cuts at: 2171 2280 2900 3775 4142 2171 Size: 109 620 875 367 4474 Fragments arranged by size: 4474 875 620 367 109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 3512 3766 5221 618 Size: 325 217 627 346 1379 254 1455 1842 Fragments arranged by size: 1842 1455 1379 627 346 325 254 217 BanII G_rGCy'C Cuts at: 1391 3238 3416 3764 1391 Size: 1847 178 348 4072 Fragments arranged by size: 4072 1847 348 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 3687 928 Size: 971 636 1152 3686 Fragments arranged by size: 3686 1152 971 636 BbvCI CC'TCA_GC Cuts at: 3285 3348 3641 3285 Size: 63 293 6089 Fragments arranged by size: 6089 293 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 3059 5056 Size: 107 122 71 631 605 547 840 1997 Cuts at: 5056 136 Size: 1525 Fragments arranged by size: 1997 1525 840 631 605 547 122 107 71 BglII A'GATC_T Cuts at: 2643 3359 6440 2643 Size: 716 3081 2648 Fragments arranged by size: 3081 2648 716 BlpI GC'TnA_GC Cuts at: 3862 3862 Size: 6445 Bme1580I G_kGCm'C Cuts at: 1790 4506 5662 1790 Size: 2716 1156 2573 Fragments arranged by size: 2716 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3412 4176 5134 Size: 416 53 83 399 24 1995 764 958 Cuts at: 5134 442 Size: 1753 Fragments arranged by size: 1995 1753 958 764 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2179 2229 2993 5105 1239 Size: 912 28 50 764 2112 2579 Fragments arranged by size: 2579 2112 912 764 50 28 Bpu10I CC'TnA_GC Cuts at: 3285 3348 3410 3641 3285 Size: 63 62 231 6089 Fragments arranged by size: 6089 231 63 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4570 5435 5733 4570 Size: 865 298 5282 Fragments arranged by size: 5282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5108 882 Size: 617 3609 2219 Fragments arranged by size: 3609 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4236 493 Size: 711 3032 2702 Fragments arranged by size: 3032 2702 711 BsaBI GATnn'nnATC Cuts at: 2357 6439 2357 Size: 4082 2363 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2023 4252 4634 5269 Size: 53 83 186 936 490 2229 382 635 Cuts at: 5269 275 Size: 1451 Fragments arranged by size: 2229 1451 936 635 490 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3422 4878 5276 5550 5697 6108 3422 Size: 1456 398 274 147 411 3759 Fragments arranged by size: 3759 1456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3120 3150 3485 3515 1178 Size: 30 165 30 1717 30 335 30 4108 Fragments arranged by size: 4108 1717 335 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 2152 3336 1186 Size: 957 9 1184 4295 Fragments arranged by size: 4295 1184 957 9 BseYI C'CCAG_C Cuts at: 2139 2749 3273 3277 3300 3564 3660 5648 6144 Size: 610 524 4 23 264 96 1988 496 Cuts at: 6144 2139 Size: 2440 Fragments arranged by size: 2440 1988 610 524 496 264 96 23 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3566 1163 Size: 573 1830 4042 Fragments arranged by size: 4042 1830 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4506 4591 5662 4506 Size: 85 1071 5289 Fragments arranged by size: 5289 1071 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3238 3416 3764 4506 4591 5662 1391 Size: 399 1448 178 348 742 85 1071 2174 Fragments arranged by size: 2174 1448 1071 742 399 348 178 85 BspEI T'CCGG_A Cuts at: 3422 6108 3422 Size: 2686 3759 BspHI T'CATG_A Cuts at: 4334 6064 4334 Size: 1730 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2085 2116 2425 2968 3800 844 Size: 1217 24 31 309 543 832 3489 Fragments arranged by size: 3489 1217 832 543 309 31 24 BsrBI CCG'CTC Cuts at: 1394 1823 4332 1394 Size: 429 2509 3507 Fragments arranged by size: 3507 2509 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5089 5276 1700 Size: 110 3279 187 2869 Fragments arranged by size: 3279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6445 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 3444 1184 Size: 2260 4185 BstXI CCAn_nnnn'nTGG Cuts at: 2897 3362 2897 Size: 465 5980 BstYI r'GATC_y Cuts at: 1972 2171 2280 2358 2468 2643 2900 3359 3425 Size: 199 109 78 110 175 257 459 66 Cuts at: 3425 3775 4142 4527 4544 5996 6082 6094 6440 Size: 350 367 385 17 1452 86 12 346 Cuts at: 6440 1972 Size: 1977 Fragments arranged by size: 1977 1452 459 385 367 350 346 257 199 175 110 109 86 78 66 17 12 Bsu36I CC'TnA_GG Cuts at: 2217 2227 3713 2217 Size: 10 1486 4949 Fragments arranged by size: 4949 1486 10 BtgI C'CryG_G Cuts at: 513 1065 2121 2329 2829 513 Size: 552 1056 208 500 4129 Fragments arranged by size: 4129 1056 552 500 208 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5706 Fragments arranged by size: 5706 683 56 BtsI GCAGTG_nn' Cuts at: 3961 4755 4775 3961 Size: 794 20 5631 Fragments arranged by size: 5631 794 20 ClaI AT'CG_AT Cuts at: 1554 2295 1554 Size: 741 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2245 2280 529 Size: 35 1681 35 4694 Fragments arranged by size: 4694 1681 35 35 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2435 3170 5452 809 Size: 507 1119 735 2282 1802 Fragments arranged by size: 2282 1802 1119 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2832 2982 Size: 54 1425 50 275 18 216 786 150 Cuts at: 2982 3159 3892 4781 6410 8 Size: 177 733 889 1629 43 Fragments arranged by size: 1629 1425 889 786 733 275 216 177 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3135 3570 4375 1353 Size: 1782 435 805 3423 Fragments arranged by size: 3423 1782 805 435 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2486 5032 5406 5552 387 Size: 1064 138 897 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 897 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2179 2229 2993 4508 5105 5892 Size: 393 519 28 50 764 1515 597 787 Cuts at: 5892 6160 1239 Size: 268 1524 Fragments arranged by size: 1524 1515 787 764 597 519 393 268 50 28 EcoNI CCTnn'n_nnAGG Cuts at: 3288 3318 3288 Size: 30 6415 EcoO109I rG'GnC_Cy Cuts at: 2212 2249 2660 3281 3326 3669 2212 Size: 37 411 621 45 343 4988 Fragments arranged by size: 4988 621 411 343 45 37 EcoRI G'AATT_C Cuts at: 2378 3754 4132 2378 Size: 1376 378 4691 Fragments arranged by size: 4691 1376 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 6445 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 3215 5592 1678 Size: 1537 2377 2531 Fragments arranged by size: 2531 2377 1537 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 2265 2297 3005 Size: 32 135 32 655 32 6 32 708 Cuts at: 3005 3037 3075 3107 5086 5118 5160 5192 1373 Size: 32 38 32 1979 32 42 32 2626 Fragments arranged by size: 2626 1979 708 655 135 42 38 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3789 4035 669 Size: 988 281 643 1208 246 3079 Fragments arranged by size: 3079 1208 988 643 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 3675 5559 5743 1305 Size: 1623 747 1884 184 2007 Fragments arranged by size: 2007 1884 1623 747 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 2834 2984 10 Size: 54 1425 343 216 786 150 3471 Fragments arranged by size: 3471 1425 786 343 216 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 4403 4762 4921 518 Size: 648 1653 1584 359 159 2042 Fragments arranged by size: 2042 1653 1584 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3018 3121 3154 3277 3861 4538 5686 5931 Size: 1412 103 33 123 584 677 1148 245 Cuts at: 5931 1606 Size: 2120 Fragments arranged by size: 2120 1412 1148 677 584 245 123 103 33 NcoI C'CATG_G Cuts at: 513 1065 2121 2329 2829 513 Size: 552 1056 208 500 4129 Fragments arranged by size: 4129 1056 552 500 208 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 2434 2507 2759 3137 3803 5348 1132 Size: 97 1205 73 252 378 666 1545 2229 Fragments arranged by size: 2229 1545 1205 666 378 252 97 73 PasI CC'CwG_GG Cuts at: 1348 2521 2619 2656 3263 1348 Size: 1173 98 37 607 4530 Fragments arranged by size: 4530 1173 607 98 37 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 2656 1108 Size: 1038 510 4897 Fragments arranged by size: 4897 1038 510 PfoI T'CCnGG_A Cuts at: 2167 2805 2167 Size: 638 5807 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2249 2660 3669 2249 Size: 411 1009 5025 Fragments arranged by size: 5025 1009 411 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PstI C_TGCA'G Cuts at: 830 2559 2612 3797 830 Size: 1729 53 1185 3478 Fragments arranged by size: 3478 1729 1185 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 3018 3154 3277 3018 Size: 136 123 6186 Fragments arranged by size: 6186 136 123 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SanDI GG'GwC_CC Cuts at: 3669 3669 Size: 6445 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2509 2555 2608 3793 4928 5609 5800 Size: 219 1464 46 53 1185 1135 681 191 Cuts at: 5800 826 Size: 1471 Fragments arranged by size: 1471 1464 1185 1135 681 219 191 53 46 SfiI GGCCn_nnn'nGGCC Cuts at: 2219 2219 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3470 3772 1872 Size: 1598 302 4545 Fragments arranged by size: 4545 1598 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 4549 5450 5712 820 Size: 197 926 2606 901 262 1553 Fragments arranged by size: 2606 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 2507 3803 1132 Size: 1375 1296 3774 Fragments arranged by size: 3774 1375 1296 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 2858 3500 1517 Size: 1341 642 4462 Fragments arranged by size: 4462 1341 642 StyI C'CwwG_G Cuts at: 513 1065 2121 2329 2829 3230 3690 3884 513 Size: 552 1056 208 500 401 460 194 3074 Fragments arranged by size: 3074 1056 552 500 460 401 208 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 2998 4472 4489 4642 4827 5272 Size: 1255 1031 387 1474 17 153 185 445 Cuts at: 5272 325 Size: 1498 Fragments arranged by size: 1498 1474 1255 1031 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4691 6403 96 Size: 274 80 33 51 1443 2714 1712 138 Fragments arranged by size: 2714 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3120 3152 3661 3693 6405 6437 3120 Size: 32 509 32 2712 32 3128 Fragments arranged by size: 3128 2712 509 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 3074 2284 Size: 790 5655 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3468 3770 1870 Size: 1598 302 4545 Fragments arranged by size: 4545 1598 302 XmnI GAAnn'nnTTC Cuts at: 2324 4574 2324 Size: 2250 4195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2024 4253 276 Size: 53 83 186 936 490 2229 2468 Fragments arranged by size: 2468 2229 936 490 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI