(Circular) (Six-base) MAPSORT of: pFN21AB4908.seq Check: 8499 from: 1 to: 6637 pFN21AB4908 6637 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2453 2515 3063 3206 3786 2453 Size: 62 548 143 580 5304 Fragments arranged by size: 5304 580 548 143 62 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2526 4447 278 Size: 53 83 186 936 990 1921 2468 Fragments arranged by size: 2468 1921 990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3980 1937 Size: 2043 4594 Acc65I G'GTAC_C Cuts at: 3958 3958 Size: 6637 AclI AA'CG_TT Cuts at: 1563 3936 4764 5137 1563 Size: 2373 828 373 3063 Fragments arranged by size: 3063 2373 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2926 3010 3346 3430 3514 3598 4700 Size: 746 548 84 336 84 84 84 1102 Cuts at: 4700 6084 6352 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1102 746 548 336 268 84 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6440 AflIII A'CryG_T Cuts at: 1203 2572 3372 5536 6513 1203 Size: 1369 800 2164 977 1327 Fragments arranged by size: 2164 1369 1327 977 800 AgeI A'CCGG_T Cuts at: 3084 3572 5468 3084 Size: 488 1896 4253 Fragments arranged by size: 4253 1896 488 AhdI GACnn_n'nnGTC Cuts at: 5366 5366 Size: 6637 AleI CACnn'nnGTG Cuts at: 1166 3573 1166 Size: 2407 4230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3056 3071 1184 Size: 1872 15 4750 Fragments arranged by size: 4750 1872 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 3233 3265 2729 Size: 32 472 32 6101 Fragments arranged by size: 6101 472 32 32 AlwNI CAG_nnn'CTG Cuts at: 3012 3264 3733 4380 5952 3012 Size: 252 469 647 1572 3697 Fragments arranged by size: 3697 1572 647 469 252 ApaI G_GGCC'C Cuts at: 2340 2340 Size: 6637 ApaLI G'TGCA_C Cuts at: 2966 3062 3386 3470 3554 4694 5850 2966 Size: 96 324 84 84 1140 1156 3753 Fragments arranged by size: 3753 1156 1140 324 96 84 84 ApoI r'AATT_y Cuts at: 1493 2537 3907 3946 4172 4324 6433 1493 Size: 1044 1370 39 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 1044 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 5191 160 Size: 872 4159 1606 Fragments arranged by size: 4159 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2081 2579 3962 1870 Size: 73 84 54 498 1383 4545 Fragments arranged by size: 4545 1383 498 84 73 54 AvrII C'CTAG_G Cuts at: 2248 2248 Size: 6637 BamHI G'GATC_C Cuts at: 2053 3844 3967 4334 2053 Size: 1791 123 367 4356 Fragments arranged by size: 4356 1791 367 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 2454 2497 2729 3958 Size: 325 217 627 578 89 43 232 1229 Cuts at: 3958 5413 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1229 627 578 325 232 217 89 43 BanII G_rGCy'C Cuts at: 1391 2028 2340 2415 2786 3710 3956 1391 Size: 637 312 75 371 924 246 4072 Fragments arranged by size: 4072 924 637 371 312 246 75 BbeI G_GCGC'C Cuts at: 2458 2501 2733 2458 Size: 43 232 6362 Fragments arranged by size: 6362 232 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 2096 928 Size: 971 140 57 5469 Fragments arranged by size: 5469 971 140 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4828 4862 1903 Size: 34 2891 34 3678 Fragments arranged by size: 3678 2891 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4529 5739 4529 Size: 1210 5427 BclI T'GATC_A Cuts at: 1548 1548 Size: 6637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5248 136 Size: 107 122 71 631 605 3576 1525 Fragments arranged by size: 3576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6632 6632 Size: 6637 BlpI GC'TnA_GC Cuts at: 3044 3782 4054 3044 Size: 738 272 5627 Fragments arranged by size: 5627 738 272 Bme1580I G_kGCm'C Cuts at: 1790 2340 2370 2970 3066 3390 3474 3558 4698 Size: 550 30 600 96 324 84 84 1140 Cuts at: 4698 5854 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1140 600 550 324 96 84 84 30 BmgBI CAC'GTC Cuts at: 1318 3841 1318 Size: 2523 4114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4368 5326 442 Size: 416 53 83 399 24 2951 958 1753 Fragments arranged by size: 2951 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3945 3945 Size: 6637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2884 2900 3724 5297 1239 Size: 1645 16 824 1573 2579 Fragments arranged by size: 2579 1645 1573 824 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4762 5627 5925 4762 Size: 865 298 5474 Fragments arranged by size: 5474 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5300 882 Size: 617 3801 2219 Fragments arranged by size: 3801 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3796 4428 493 Size: 711 2592 632 2702 Fragments arranged by size: 2702 2592 711 632 BsaBI GATnn'nnATC Cuts at: 6631 6631 Size: 6637 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2455 2498 2523 2730 Size: 53 83 186 936 922 43 25 207 Cuts at: 2730 4444 4826 5461 275 Size: 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 922 635 382 207 186 83 53 43 25 BsaWI w'CCGG_w Cuts at: 3084 3572 5070 5468 5742 5889 6300 3084 Size: 488 1498 398 274 147 411 3421 Fragments arranged by size: 3421 1498 488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2729 2759 3653 3683 1178 Size: 30 165 30 1326 30 894 30 4132 Fragments arranged by size: 4132 1326 894 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2315 2491 2776 1186 Size: 997 132 176 285 5047 Fragments arranged by size: 5047 997 285 176 132 BseYI C'CCAG_C Cuts at: 2565 3811 5840 6336 2565 Size: 1246 2029 496 2866 Fragments arranged by size: 2866 2029 1246 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 2679 2783 3025 3277 3613 3719 Size: 573 510 433 104 242 252 336 106 Cuts at: 3719 3837 1163 Size: 118 3963 Fragments arranged by size: 3963 573 510 433 336 252 242 118 106 104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4087 4848 5876 1540 Size: 275 186 2086 761 1028 2301 Fragments arranged by size: 2301 2086 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2470 2786 2870 2886 2970 3066 3390 3474 3558 Size: 316 84 16 84 96 324 84 84 Cuts at: 3558 3710 3726 4698 4783 5854 2470 Size: 152 16 972 85 1071 3253 Fragments arranged by size: 3253 1071 972 324 316 152 96 85 84 84 84 84 16 16 BsmI GAATG_Cn' Cuts at: 2849 4148 4241 2849 Size: 1299 93 5245 Fragments arranged by size: 5245 1299 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2340 2370 2415 2470 2786 2870 Size: 399 238 312 30 45 55 316 84 Cuts at: 2870 2886 2970 3066 3390 3474 3558 3710 3726 Size: 16 84 96 324 84 84 152 16 Cuts at: 3726 3956 4698 4783 5854 1391 Size: 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 316 312 238 230 152 96 85 84 84 84 84 55 45 30 16 16 BspEI T'CCGG_A Cuts at: 6300 6300 Size: 6637 BspHI T'CATG_A Cuts at: 4526 6256 4526 Size: 1730 4907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2453 2515 3063 3206 3387 3786 3992 844 Size: 1609 62 548 143 181 399 206 3489 Fragments arranged by size: 3489 1609 548 399 206 181 143 62 BsrBI CCG'CTC Cuts at: 1394 1823 2425 2992 3288 4524 1394 Size: 429 602 567 296 1236 3507 Fragments arranged by size: 3507 1236 602 567 429 296 BsrDI GCAATG_nn' Cuts at: 65 1248 5132 5306 65 Size: 1183 3884 174 1396 Fragments arranged by size: 3884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2401 2712 2802 2886 2900 3068 3084 Size: 110 591 311 90 84 14 168 16 Cuts at: 3084 3390 3474 3572 5281 5468 1700 Size: 306 84 98 1709 187 2869 Fragments arranged by size: 2869 1709 591 311 306 187 168 110 98 90 84 84 16 14 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6637 BssSI C'ACGA_G Cuts at: 3383 4697 5709 3383 Size: 1314 1012 4311 Fragments arranged by size: 4311 1314 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3012 3264 3432 3600 3824 794 Size: 2218 252 168 168 224 3607 Fragments arranged by size: 3607 2218 252 224 168 168 BstBI TT'CG_AA Cuts at: 5441 5441 Size: 6637 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6637 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2878 Size: 6637 BstYI r'GATC_y Cuts at: 1972 2010 2053 3228 3844 3967 4334 4719 4736 Size: 38 43 1175 616 123 367 385 17 Cuts at: 4736 6188 6274 6286 6632 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1175 616 385 367 346 123 86 43 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2546 2546 Size: 6637 BtgI C'CryG_G Cuts at: 513 1065 2004 2306 2511 513 Size: 552 939 302 205 4639 Fragments arranged by size: 4639 939 552 302 205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2853 487 Size: 683 56 1627 4271 Fragments arranged by size: 4271 1627 683 56 BtsI GCAGTG_nn' Cuts at: 2258 4153 4947 4967 2258 Size: 1895 794 20 3928 Fragments arranged by size: 3928 1895 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6602 DraI TTT'AAA Cuts at: 3941 3941 Size: 6637 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5644 809 Size: 507 4328 1802 Fragments arranged by size: 4328 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3756 4084 4973 Size: 54 1425 50 275 18 1926 328 889 Cuts at: 4973 6602 8 Size: 1629 43 Fragments arranged by size: 1926 1629 1425 889 328 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4084 1537 Size: 275 2272 4090 Fragments arranged by size: 4090 2272 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 4567 1353 Size: 1211 2003 3423 Fragments arranged by size: 3423 2003 1211 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 5224 5598 5744 387 Size: 1064 138 1176 2459 374 146 1280 Fragments arranged by size: 2459 1280 1176 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2784 3708 2784 Size: 924 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2884 2900 2926 3010 3346 3430 Size: 393 746 506 16 26 84 336 84 Cuts at: 3430 3514 3598 3724 4700 5297 6084 6352 1239 Size: 84 84 126 976 597 787 268 1524 Fragments arranged by size: 1524 976 787 746 597 506 393 336 268 126 84 84 84 84 26 16 EcoO109I rG'GnC_Cy Cuts at: 2199 2252 2336 2337 2588 2641 2199 Size: 53 84 1 251 53 6195 Fragments arranged by size: 6195 251 84 53 53 1 EcoRI G'AATT_C Cuts at: 2537 3946 4324 2537 Size: 1409 378 4850 Fragments arranged by size: 4850 1409 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 6637 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 2458 2501 2733 2809 3012 5784 1678 Size: 780 43 232 76 203 2772 2531 Fragments arranged by size: 2772 2531 780 232 203 76 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2264 2269 2296 Size: 32 135 32 503 32 157 5 27 Cuts at: 2296 2301 5278 5310 5352 5384 1373 Size: 5 2977 32 42 32 2626 Fragments arranged by size: 2977 2626 503 157 135 42 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2180 3981 4227 669 Size: 988 281 242 1801 246 3079 Fragments arranged by size: 3079 1801 988 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 6637 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KasI G'GCGC_C Cuts at: 2454 2497 2729 2454 Size: 43 232 6362 Fragments arranged by size: 6362 232 43 KpnI G_GTAC'C Cuts at: 3962 3962 Size: 6637 MfeI C'AATT_G Cuts at: 4236 4236 Size: 6637 MluI A'CGCG_T Cuts at: 2572 3372 6513 2572 Size: 800 3141 2696 Fragments arranged by size: 3141 2696 800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 3500 5751 5935 1305 Size: 897 1298 2251 184 2007 Fragments arranged by size: 2251 2007 1298 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3758 10 Size: 54 1425 343 1926 2889 Fragments arranged by size: 2889 1926 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3573 4595 4954 5113 518 Size: 648 2407 1022 359 159 2042 Fragments arranged by size: 2407 2042 1022 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2946 3030 3237 3366 3405 3534 4053 4730 Size: 1340 84 207 129 39 129 519 677 Cuts at: 4730 5878 6123 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1340 1148 677 519 245 207 129 129 84 39 NaeI GCC'GGC Cuts at: 2714 2714 Size: 6637 NarI GG'CG_CC Cuts at: 2455 2498 2730 2455 Size: 43 232 6362 Fragments arranged by size: 6362 232 43 NcoI C'CATG_G Cuts at: 513 1065 2004 2306 2511 513 Size: 552 939 302 205 4639 Fragments arranged by size: 4639 939 552 302 205 NdeI CA'TA_TG Cuts at: 387 387 Size: 6637 NgoMIV G'CCGG_C Cuts at: 2712 2712 Size: 6637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 2317 3052 3995 5540 1132 Size: 97 1088 735 943 1545 2229 Fragments arranged by size: 2229 1545 1088 943 735 97 PasI CC'CwG_GG Cuts at: 1348 2493 3916 1348 Size: 1145 1423 4069 Fragments arranged by size: 4069 1423 1145 PciI A'CATG_T Cuts at: 5536 5536 Size: 6637 PflMI CCAn_nnn'nTGG Cuts at: 1108 2517 1108 Size: 1409 5228 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2729 2761 4706 4738 6219 6251 2729 Size: 32 1945 32 1481 32 3115 Fragments arranged by size: 3115 1945 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2252 2588 2252 Size: 336 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 4207 4207 Size: 6637 PspOMI G'GGCC_C Cuts at: 2336 2336 Size: 6637 PstI C_TGCA'G Cuts at: 830 2475 3035 3512 3989 830 Size: 1645 560 477 477 3478 Fragments arranged by size: 3478 1645 560 477 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 PvuII CAG'CTG Cuts at: 2946 3030 3366 3534 2946 Size: 84 336 168 6049 Fragments arranged by size: 6049 336 168 84 SacI G_AGCT'C Cuts at: 2786 3710 2786 Size: 924 5713 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SanDI GG'GwC_CC Cuts at: 2588 2588 Size: 6637 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 4885 6597 4885 Size: 1712 4925 SfcI C'TryA_G Cuts at: 826 1045 2471 2529 3031 3508 3985 5120 5801 Size: 219 1426 58 502 477 477 1135 681 Cuts at: 5801 5992 826 Size: 191 1471 Fragments arranged by size: 1471 1426 1135 681 502 477 477 219 191 58 SfoI GGC'GCC Cuts at: 2456 2499 2731 2456 Size: 43 232 6362 Fragments arranged by size: 6362 232 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 2802 2886 2900 3068 3390 3474 3572 1700 Size: 1102 84 14 168 322 84 98 4765 Fragments arranged by size: 4765 1102 322 168 98 84 84 14 SmaI CCC'GGG Cuts at: 1872 3964 1872 Size: 2092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 4741 5642 5904 820 Size: 197 926 138 2660 901 262 1553 Fragments arranged by size: 2660 1553 926 901 262 197 138 SnaBI TAC'GTA Cuts at: 493 4428 493 Size: 3935 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6637 SphI G_CATG'C Cuts at: 1132 2317 3052 3995 1132 Size: 1185 735 943 3774 Fragments arranged by size: 3774 1185 943 735 SspI AAT'ATT Cuts at: 5 52 4561 5 Size: 47 4509 2081 Fragments arranged by size: 4509 2081 47 StuI AGG'CCT Cuts at: 1517 2855 2939 3023 3107 3191 3275 3527 1517 Size: 1338 84 84 84 84 84 252 4627 Fragments arranged by size: 4627 1338 252 84 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2248 2306 2511 2637 3753 Size: 552 939 117 127 58 205 126 1116 Cuts at: 3753 4076 513 Size: 323 3074 Fragments arranged by size: 3074 1116 939 552 323 205 127 126 117 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 3803 4664 4681 4834 5019 5464 Size: 1255 597 1626 861 17 153 185 445 Cuts at: 5464 325 Size: 1498 Fragments arranged by size: 1626 1498 1255 861 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4883 6595 96 Size: 274 80 33 51 1443 2906 1712 138 Fragments arranged by size: 2906 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6597 6629 6597 Size: 32 6605 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3973 3973 Size: 6637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3856 3856 Size: 6637 XhoI C'TCGA_G Cuts at: 1943 2081 1943 Size: 138 6499 XmaI C'CCGG_G Cuts at: 1870 3962 1870 Size: 2092 4545 XmnI GAAnn'nnTTC Cuts at: 2771 2912 2939 3023 3275 3332 3359 3416 3500 Size: 141 27 84 252 57 27 57 84 Cuts at: 3500 3527 4766 2771 Size: 27 1239 4642 Fragments arranged by size: 4642 1239 252 141 84 84 57 57 27 27 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2524 4445 276 Size: 53 83 186 936 990 1921 2468 Fragments arranged by size: 2468 1921 990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI