(Circular) (Six-base) MAPSORT of: pFN21AB4878.seq Check: 9462 from: 1 to: 6238 pFN21AB4878 6238 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 2567 2957 1166 Size: 1401 390 4447 Fragments arranged by size: 4447 1401 390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 3247 3981 5553 3247 Size: 734 1572 3932 Fragments arranged by size: 3932 1572 734 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2557 2621 3547 3773 3925 6034 1493 Size: 1064 64 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 926 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3563 1870 Size: 73 1620 4545 Fragments arranged by size: 4545 1620 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3303 3344 3559 5014 618 Size: 325 217 627 1516 41 215 1455 1842 Fragments arranged by size: 1842 1516 1455 627 325 217 215 41 BanII G_rGCy'C Cuts at: 1391 2769 2937 3557 1391 Size: 1378 168 620 4072 Fragments arranged by size: 4072 1378 620 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2680 2810 3370 928 Size: 971 781 130 560 3796 Fragments arranged by size: 3796 971 781 560 130 BbvCI CC'TCA_GC Cuts at: 2552 2552 Size: 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 3315 1548 Size: 1767 4471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 2080 3655 2080 Size: 1575 4663 Bme1580I G_kGCm'C Cuts at: 1790 3306 4299 5455 1790 Size: 1516 993 1156 2573 Fragments arranged by size: 2573 1516 1156 993 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 3969 4927 Size: 416 53 83 399 24 1892 660 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1892 1753 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 4898 1239 Size: 1280 2379 2579 Fragments arranged by size: 2579 2379 1280 Bpu10I CC'TnA_GC Cuts at: 2552 2552 Size: 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3286 4363 5228 5526 3286 Size: 1077 865 298 3998 Fragments arranged by size: 3998 1077 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4901 882 Size: 617 1158 2244 2219 Fragments arranged by size: 2244 2219 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 4427 5062 275 Size: 53 83 186 936 2512 382 635 1451 Fragments arranged by size: 2512 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2691 4671 5069 5343 5490 5901 2691 Size: 1980 398 274 147 411 3028 Fragments arranged by size: 3028 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6013 Fragments arranged by size: 6013 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 1186 Size: 1000 5238 BseYI C'CCAG_C Cuts at: 2140 2249 2574 3331 5441 5937 2140 Size: 109 325 757 2110 496 2441 Fragments arranged by size: 2441 2110 757 496 325 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2540 1163 Size: 573 804 4861 Fragments arranged by size: 4861 804 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 2769 2781 4299 4384 5455 2727 Size: 42 12 1518 85 1071 3510 Fragments arranged by size: 3510 1518 1071 85 42 12 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2727 2769 2781 2937 3306 3557 4299 Size: 399 937 42 12 156 369 251 742 Cuts at: 4299 4384 5455 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 399 369 251 156 85 42 12 BspEI T'CCGG_A Cuts at: 2691 5901 2691 Size: 3210 3028 BspHI T'CATG_A Cuts at: 2193 4127 5857 2193 Size: 1934 1730 2574 Fragments arranged by size: 2574 1934 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3593 844 Size: 2749 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 4125 1394 Size: 429 206 2096 3507 Fragments arranged by size: 3507 2096 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 4733 4907 65 Size: 1183 822 2663 174 1396 Fragments arranged by size: 2663 1396 1183 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2755 4882 5069 1700 Size: 110 945 2127 187 2869 Fragments arranged by size: 2869 2127 945 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 2569 1184 Size: 1385 4853 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2351 2896 2923 2959 3292 2011 Size: 340 545 27 36 333 4957 Fragments arranged by size: 4957 545 340 333 36 27 BstYI r'GATC_y Cuts at: 1972 2885 3568 3935 4320 4337 5789 5875 5887 Size: 913 683 367 385 17 1452 86 12 Cuts at: 5887 6233 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 913 683 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3410 3410 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2928 2952 3154 3309 513 Size: 552 1863 24 202 155 3442 Fragments arranged by size: 3442 1863 552 202 155 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2890 487 Size: 683 56 1664 3835 Fragments arranged by size: 3835 1664 683 56 BtsI GCAGTG_nn' Cuts at: 2401 3754 4548 4568 2401 Size: 1353 794 20 4071 Fragments arranged by size: 4071 1353 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 2178 2925 Size: 54 1425 50 275 18 200 148 747 Cuts at: 2925 2995 3151 3294 3429 3685 4574 6203 8 Size: 70 156 143 135 256 889 1629 43 Fragments arranged by size: 1629 1425 889 747 275 256 200 156 148 143 135 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2784 3494 4168 1353 Size: 1431 710 674 3423 Fragments arranged by size: 3423 1431 710 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2767 2767 Size: 6238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 4301 4898 5685 5953 1239 Size: 393 887 1782 597 787 268 1524 Fragments arranged by size: 1782 1524 887 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2232 3414 2232 Size: 1182 5056 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 5385 1678 Size: 3707 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2670 2702 2870 Size: 32 135 32 434 32 632 32 168 Cuts at: 2870 2902 4879 4911 4953 4985 1373 Size: 32 1977 32 42 32 2626 Fragments arranged by size: 2626 1977 632 434 168 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3441 5352 5536 1305 Size: 2136 1911 184 2007 Fragments arranged by size: 2136 2007 1911 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 2927 2997 3296 3431 Size: 54 1425 343 348 747 70 299 135 Cuts at: 3431 10 Size: 2817 Fragments arranged by size: 2817 1425 747 348 343 299 135 70 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2343 2567 2921 2957 3290 4196 Size: 648 843 334 224 354 36 333 906 Cuts at: 4196 4555 4714 518 Size: 359 159 2042 Fragments arranged by size: 2042 906 843 648 359 354 334 333 224 159 36 MspA1I CmG'CkG Cuts at: 1606 3427 3654 4331 5479 5724 1606 Size: 1821 227 677 1148 245 2120 Fragments arranged by size: 2120 1821 1148 677 245 227 NcoI C'CATG_G Cuts at: 513 1065 2928 3154 3309 513 Size: 552 1863 226 155 3442 Fragments arranged by size: 3442 1863 552 226 155 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 2257 2701 3596 5141 1132 Size: 97 1028 444 895 1545 2229 Fragments arranged by size: 2229 1545 1028 895 444 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PfoI T'CCnGG_A Cuts at: 3197 3197 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2232 3414 2232 Size: 1182 5056 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3590 830 Size: 2760 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 3427 3427 Size: 6238 SacI G_AGCT'C Cuts at: 2769 2769 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SexAI A'CCwGG_T Cuts at: 2973 3240 2973 Size: 267 5971 SfcI C'TryA_G Cuts at: 826 1045 2392 3279 3586 4721 5402 5593 826 Size: 219 1347 887 307 1135 681 191 1471 Fragments arranged by size: 1471 1347 1135 887 681 307 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3265 4342 5243 5505 820 Size: 197 926 1322 1077 901 262 1553 Fragments arranged by size: 1553 1322 1077 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 2257 2701 3596 1132 Size: 1125 444 895 3774 Fragments arranged by size: 3774 1125 895 444 SspI AAT'ATT Cuts at: 5 52 2305 4162 5 Size: 47 2253 1857 2081 Fragments arranged by size: 2253 2081 1857 47 StuI AGG'CCT Cuts at: 1517 2398 1517 Size: 881 5357 StyI C'CwwG_G Cuts at: 513 1065 2166 2423 2928 3039 3154 3309 3677 Size: 552 1101 257 505 111 115 155 368 Cuts at: 3677 513 Size: 3074 Fragments arranged by size: 3074 1101 552 505 368 257 155 115 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2263 2469 4265 4282 4435 4620 5065 Size: 1255 683 206 1796 17 153 185 445 Cuts at: 5065 325 Size: 1498 Fragments arranged by size: 1796 1498 1255 683 445 206 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3447 4484 6196 Size: 274 80 33 51 1443 1470 1037 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1712 1470 1443 1037 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 2684 1316 Size: 1368 4870 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2173 2173 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI