Restriction map for
pFN21AB4862
(Circular) (Six-base) MAPSORT of: pFN21AB4862.seq Check: 6606 from: 1 to: 6160
pFN21AB4862 6160 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3441 3970 278
Size: 53 83 186 936 1905 529 2468
Fragments arranged by size:
2468 1905 936 529 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2415 3503 1937
Size: 478 1088 4594
Fragments arranged by size:
4594 1088 478
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4223 5607 5875 1632
Size: 2591 1384 268 1917
Fragments arranged by size:
2591 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 2841 3366 5059 6036 1203
Size: 1638 525 1693 977 1327
Fragments arranged by size:
1693 1638 1327 977 525
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2467 1184
Size: 1283 4877
AlwNI CAG_nnn'CTG
Cuts at: 3397 3903 5475 3397
Size: 506 1572 4082
Fragments arranged by size:
4082 1572 506
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 2396 3221 3469 3695 3847 5956 1493
Size: 903 825 248 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 825 248 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2873 3485 1870
Size: 73 534 396 612 4545
Fragments arranged by size:
4545 612 534 396 73
BamHI G'GATC_C
Cuts at: 2328 3490 3857 2328
Size: 1162 367 4631
Fragments arranged by size:
4631 1162 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3041 3481 4936 618
Size: 325 217 627 1254 440 1455 1842
Fragments arranged by size:
1842 1455 1254 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2670 2724 3479 1391
Size: 1279 54 755 4072
Fragments arranged by size:
4072 1279 755 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4771 136
Size: 107 122 71 631 605 3099 1525
Fragments arranged by size:
3099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 3044 4221 5377 1790
Size: 1254 1177 1156 2573
Fragments arranged by size:
2573 1254 1177 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2333 2383 2632
Size: 416 53 83 399 24 916 50 249
Cuts at: 2632 2926 3891 4849 442
Size: 294 965 958 1753
Fragments arranged by size:
1753 965 958 916 416 399 294 249
83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2743 2792 2465
Size: 278 49 5833
Fragments arranged by size:
5833 278 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 2959 4820 1239
Size: 1523 197 1861 2579
Fragments arranged by size:
2579 1861 1523 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 2717 4285 5150 5448 2259
Size: 458 1568 865 298 2971
Fragments arranged by size:
2971 1568 865 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2763 4823 882
Size: 617 1264 2060 2219
Fragments arranged by size:
2219 2060 1264 617
BsaAI yAC'GTr
Cuts at: 493 1204 3367 3951 493
Size: 711 2163 584 2702
Fragments arranged by size:
2702 2163 711 584
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3438 3967 4349 4984
Size: 53 83 186 936 1905 529 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1905 1451 936 635 529 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2845 2875 1178
Size: 30 165 30 1442 30 4463
Fragments arranged by size:
4463 1442 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2714 3431 1186
Size: 1528 717 3915
Fragments arranged by size:
3915 1528 717
BseYI C'CCAG_C
Cuts at: 2027 2575 2669 3216 3400 5363 5859 2027
Size: 548 94 547 184 1963 496 2328
Fragments arranged by size:
2328 1963 548 547 496 184 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2250 3407 1163
Size: 573 514 1157 3916
Fragments arranged by size:
3916 1157 573 514
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 4221 4306 5377 2724
Size: 1497 85 1071 3507
Fragments arranged by size:
3507 1497 1071 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 2724 3044 3479 4221 4306 5377
Size: 399 880 54 320 435 742 85 1071
Cuts at: 5377 1391
Size: 2174
Fragments arranged by size:
2174 1071 880 742 435 399 320 85
54
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3396 3515 844
Size: 2552 119 3489
Fragments arranged by size:
3489 2552 119
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4804 4991 1700
Size: 110 2994 187 2869
Fragments arranged by size:
2994 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3397 3430 794
Size: 2603 33 3524
Fragments arranged by size:
3524 2603 33
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2101 2328 2526 3490 3857 4242 4259 5711
Size: 129 227 198 964 367 385 17 1452
Cuts at: 5711 5797 5809 6155 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 964 385 367 346 227 198
129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3414 3414
Size: 6160
BtgI C'CryG_G
Cuts at: 513 1065 3307 513
Size: 552 2242 3366
Fragments arranged by size:
3366 2242 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 2073 2354 3136 3676 4470 4490 2073
Size: 281 782 540 794 20 3743
Fragments arranged by size:
3743 794 782 540 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3092 5167 809
Size: 507 1776 2075 1802
Fragments arranged by size:
2075 1802 1776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3249 3607 4496
Size: 54 1425 50 275 18 1419 358 889
Cuts at: 4496 6125 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1419 889 358 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2712 4090 1353
Size: 1359 1378 3423
Fragments arranged by size:
3423 1378 1359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 6160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 2959 4223 4820 5607 5875 1239
Size: 393 1130 197 1264 597 787 268 1524
Fragments arranged by size:
1524 1264 1130 787 597 393 268 197
EcoNI CCTnn'n_nnAGG
Cuts at: 3080 3080
Size: 6160
EcoO109I rG'GnC_Cy
Cuts at: 2081 2081
Size: 6160
EcoRI G'AATT_C
Cuts at: 2396 3221 3469 3847 2396
Size: 825 248 378 4709
Fragments arranged by size:
4709 825 378 248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 6160
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2057 5307 1678
Size: 379 3250 2531
Fragments arranged by size:
3250 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2313 2345 2686
Size: 32 135 32 592 32 117 32 341
Cuts at: 2686 2718 2738 2770 2845 2877 4801 4833 4875
Size: 32 20 32 75 32 1924 32 42
Cuts at: 4875 4907 1373
Size: 32 2626
Fragments arranged by size:
2626 1924 592 341 135 117 75 42
32 32 32 32 32 32 32 32
32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 3504 3750 669
Size: 988 281 634 932 246 3079
Fragments arranged by size:
3079 988 932 634 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3251 10
Size: 54 1425 343 1419 2919
Fragments arranged by size:
2919 1425 1419 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2846 4118 4477 4636 518
Size: 648 1680 1272 359 159 2042
Fragments arranged by size:
2042 1680 1272 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3145 3201 3353 3576 4253 5401 5646 1606
Size: 1539 56 152 223 677 1148 245 2120
Fragments arranged by size:
2120 1539 1148 677 245 223 152 56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5608
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2636 2845 3518 5063 1132
Size: 97 1407 209 673 1545 2229
Fragments arranged by size:
2229 1545 1407 673 209 97
PasI CC'CwG_GG
Cuts at: 1348 2458 1348
Size: 1110 5050
PciI A'CATG_T
Cuts at: 2841 5059 2841
Size: 2218 3942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3258 1108
Size: 2150 4010
PfoI T'CCnGG_A
Cuts at: 2936 2936
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 6160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2068 2535 2970 3393 3512 830
Size: 1238 467 435 423 119 3478
Fragments arranged by size:
3478 1238 467 435 423 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 3145 3201 3145
Size: 56 6104
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SapI GCTCTTCn'nnn_
Cuts at: 2712 2712
Size: 6160
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2064 2531 2966 3389 3508 4643 5324
Size: 219 1019 467 435 423 119 1135 681
Cuts at: 5324 5515 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1019 681 467 435 423 219
191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 2477 2732 4264 5165 5427
Size: 197 926 331 203 255 1532 901 262
Cuts at: 5427 820
Size: 1553
Fragments arranged by size:
1553 1532 926 901 331 262 255 203
197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 2636 3518 1132
Size: 1504 882 3774
Fragments arranged by size:
3774 1504 882
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 3036 3599 513
Size: 552 1971 563 3074
Fragments arranged by size:
3074 1971 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2365 2746 2945 4187 4204 4357 4542
Size: 1255 785 381 199 1242 17 153 185
Cuts at: 4542 4987 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1242 785 445 381 199 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4406 6118 96
Size: 274 80 33 51 1443 2429 1712 138
Fragments arranged by size:
2429 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2848 2880 6120 6152 2848
Size: 32 3240 32 2856
Fragments arranged by size:
3240 2856 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 2521 3496 2521
Size: 975 5185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3144 3144
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 2477 1943
Size: 534 5626
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2396 4289 2396
Size: 1893 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3439 3968 276
Size: 53 83 186 936 1905 529 2468
Fragments arranged by size:
2468 1905 936 529 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI