(Circular) (Six-base) MAPSORT of: pFN21AB4822.seq Check: 8698 from: 1 to: 6058 pFN21AB4822 6058 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4121 5505 5773 1632 Size: 2489 1384 268 1917 Fragments arranged by size: 2489 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3132 1184 Size: 1948 4110 AlwNI CAG_nnn'CTG Cuts at: 2086 2875 3801 5373 2086 Size: 789 926 1572 2771 Fragments arranged by size: 2771 1572 926 789 ApaI G_GGCC'C Cuts at: 3021 3021 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 3367 3593 3745 5854 1493 Size: 1874 226 152 2109 1697 Fragments arranged by size: 2109 1874 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2816 3383 1870 Size: 73 174 699 567 4545 Fragments arranged by size: 4545 699 567 174 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2976 3021 3377 1391 Size: 1585 45 356 4072 Fragments arranged by size: 4072 1585 356 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2291 928 Size: 971 392 4695 Fragments arranged by size: 4695 971 392 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2820 4669 136 Size: 107 122 71 631 605 1148 1849 1525 Fragments arranged by size: 1849 1525 1148 631 605 122 107 71 BglII A'GATC_T Cuts at: 3329 6053 3329 Size: 2724 3334 BlpI GC'TnA_GC Cuts at: 2927 3475 2927 Size: 548 5510 Bme1580I G_kGCm'C Cuts at: 1790 2230 2677 3021 4119 5275 1790 Size: 440 447 344 1098 1156 2573 Fragments arranged by size: 2573 1156 1098 447 440 344 BmgBI CAC'GTC Cuts at: 1318 2808 1318 Size: 1490 4568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3019 3789 4747 Size: 416 53 83 399 24 1602 770 958 Cuts at: 4747 442 Size: 1753 Fragments arranged by size: 1753 1602 958 770 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3233 1056 Size: 2177 3881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2447 2557 2957 3074 4718 1239 Size: 794 414 110 400 117 1644 2579 Fragments arranged by size: 2579 1644 794 414 400 117 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3054 4721 882 Size: 617 1555 1667 2219 Fragments arranged by size: 2219 1667 1555 617 BsaAI yAC'GTr Cuts at: 493 1204 2847 3217 3849 493 Size: 711 1643 370 632 2702 Fragments arranged by size: 2702 1643 711 632 370 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2205 2853 3865 4247 Size: 53 83 186 936 672 648 1012 382 Cuts at: 4247 4882 275 Size: 635 1451 Fragments arranged by size: 1451 1012 936 672 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 1178 Size: 30 165 30 1713 30 4090 Fragments arranged by size: 4090 1713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2213 3185 1186 Size: 967 60 972 4059 Fragments arranged by size: 4059 972 967 60 BseYI C'CCAG_C Cuts at: 2488 2493 2626 5261 5757 2488 Size: 5 133 2635 496 2789 Fragments arranged by size: 2789 2635 496 133 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2744 1163 Size: 573 1008 4477 Fragments arranged by size: 4477 1008 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 2530 3040 3130 3508 4269 Size: 275 186 25 504 510 90 378 761 Cuts at: 4269 5297 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 510 504 378 275 186 90 25 BsiHKAI G_wGCw'C Cuts at: 4119 4204 5275 4119 Size: 85 1071 4902 Fragments arranged by size: 4902 1071 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2230 2677 2976 3021 3377 4119 4204 Size: 399 440 447 299 45 356 742 85 Cuts at: 4204 5275 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 440 399 356 299 85 45 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3077 3413 844 Size: 1644 589 336 3489 Fragments arranged by size: 3489 1644 589 336 BsrBI CCG'CTC Cuts at: 1394 1823 2341 3132 3945 1394 Size: 429 518 791 813 3507 Fragments arranged by size: 3507 813 791 518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2323 4553 4727 65 Size: 1183 1075 2230 174 1396 Fragments arranged by size: 2230 1396 1183 1075 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2754 3039 4702 4889 1700 Size: 110 944 285 1663 187 2869 Fragments arranged by size: 2869 1663 944 285 187 110 BsrGI T'GTAC_A Cuts at: 96 2376 96 Size: 2280 3778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 2789 4118 5130 2789 Size: 1329 1012 3717 Fragments arranged by size: 3717 1329 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2863 3052 2011 Size: 852 189 5017 Fragments arranged by size: 5017 852 189 BstYI r'GATC_y Cuts at: 1972 2829 3003 3329 3388 3755 4140 4157 5609 Size: 857 174 326 59 367 385 17 1452 Cuts at: 5609 5695 5707 6053 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 857 385 367 346 326 174 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2391 3278 2391 Size: 887 5171 BtgI C'CryG_G Cuts at: 513 1065 2605 2836 3124 513 Size: 552 1540 231 288 3447 Fragments arranged by size: 3447 1540 552 288 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3574 4368 4388 3574 Size: 794 20 5244 Fragments arranged by size: 5244 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 2236 3362 2236 Size: 1126 4932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2608 2899 Size: 54 1425 50 275 18 193 585 291 Cuts at: 2899 3127 3505 4394 6023 8 Size: 228 378 889 1629 43 Fragments arranged by size: 1629 1425 889 585 378 291 275 228 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2023 3127 3505 1537 Size: 275 211 1104 378 4090 Fragments arranged by size: 4090 1104 378 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2219 3988 1353 Size: 785 81 1769 3423 Fragments arranged by size: 3423 1769 785 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2046 2066 3326 4645 5019 5165 Size: 1064 138 457 20 1260 1319 374 146 Cuts at: 5165 387 Size: 1280 Fragments arranged by size: 1319 1280 1260 1064 457 374 146 138 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2447 2557 2957 3074 4121 4718 Size: 393 401 414 110 400 117 1047 597 Cuts at: 4718 5505 5773 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 787 597 414 401 400 393 268 117 110 EcoNI CCTnn'n_nnAGG Cuts at: 2792 2867 2792 Size: 75 5983 EcoO109I rG'GnC_Cy Cuts at: 2431 2872 3018 2431 Size: 441 146 5471 Fragments arranged by size: 5471 441 146 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 3029 5205 1678 Size: 1351 2176 2531 Fragments arranged by size: 2531 2176 1351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 4699 4731 4773 Size: 32 135 32 669 32 2426 32 42 Cuts at: 4773 4805 1373 Size: 32 2626 Fragments arranged by size: 2626 2426 669 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5172 5356 1305 Size: 3867 184 2007 Fragments arranged by size: 3867 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2901 10 Size: 54 1425 343 1069 3167 Fragments arranged by size: 3167 1425 1069 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2847 4016 4375 4534 518 Size: 648 1681 1169 359 159 2042 Fragments arranged by size: 2042 1681 1169 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2350 2607 3474 4151 5299 5544 1606 Size: 500 244 257 867 677 1148 245 2120 Fragments arranged by size: 2120 1148 867 677 500 257 245 244 NaeI GCC'GGC Cuts at: 2756 2756 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 2836 513 Size: 552 1771 3735 Fragments arranged by size: 3735 1771 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NgoMIV G'CCGG_C Cuts at: 2754 2754 Size: 6058 NheI G'CTAG_C Cuts at: 1052 3229 1052 Size: 2177 3881 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 3416 4961 1132 Size: 97 2187 1545 2229 Fragments arranged by size: 2229 2187 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6058 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6058 PfoI T'CCnGG_A Cuts at: 2550 2695 2550 Size: 145 5913 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2872 2431 Size: 441 5617 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 3017 3017 Size: 6058 PstI C_TGCA'G Cuts at: 830 3410 830 Size: 2580 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 PvuII CAG'CTG Cuts at: 2350 2350 Size: 6058 SacII CC_GC'GG Cuts at: 2608 2608 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SapI GCTCTTCn'nnn_ Cuts at: 2138 2219 2138 Size: 81 5977 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SexAI A'CCwGG_T Cuts at: 2868 2868 Size: 6058 SfcI C'TryA_G Cuts at: 826 1045 3406 4541 5222 5413 826 Size: 219 2361 1135 681 191 1471 Fragments arranged by size: 2361 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2119 3385 1872 Size: 247 1266 4545 Fragments arranged by size: 4545 1266 247 SmlI C'TyrA_G Cuts at: 820 1017 1943 4162 5063 5325 820 Size: 197 926 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2847 3849 493 Size: 2354 1002 2702 Fragments arranged by size: 2702 2354 1002 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2736 2836 3497 513 Size: 552 1671 100 661 3074 Fragments arranged by size: 3074 1671 661 552 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2544 4085 4102 4255 4440 4885 325 Size: 1255 964 1541 17 153 185 445 1498 Fragments arranged by size: 1541 1498 1255 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 2772 4304 Size: 274 80 33 51 1443 399 396 1532 Cuts at: 4304 6016 96 Size: 1712 138 Fragments arranged by size: 1712 1532 1443 399 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3116 3148 6018 6050 3116 Size: 32 2870 32 3124 Fragments arranged by size: 3124 2870 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2546 2806 2870 1316 Size: 1230 260 64 4504 Fragments arranged by size: 4504 1230 260 64 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 2117 3383 1870 Size: 247 1266 4545 Fragments arranged by size: 4545 1266 247 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI