Restriction map for
pFN21AB4822
(Linear) (Six-base) MAPSORT of: KIBB4822.seq Check: 4902 from: 1 to: 1353
KIBB4822.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1127 1353
Size: 1127 226
AlwNI CAG_nnn'CTG
Cuts at: 0 81 870 1353
Size: 81 789 483
Fragments arranged by size:
789 483 81
ApaI G_GGCC'C
Cuts at: 0 1016 1353
Size: 1016 337
AvaI C'yCGr_G
Cuts at: 0 112 811 1353
Size: 112 699 542
Fragments arranged by size:
699 542 112
BanII G_rGCy'C
Cuts at: 0 971 1016 1353
Size: 971 45 337
Fragments arranged by size:
971 337 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 1353
Size: 286 1067
BglI GCCn_nnn'nGGC
Cuts at: 0 815 1353
Size: 815 538
BglII A'GATC_T
Cuts at: 0 1324 1353
Size: 1324 29
BlpI GC'TnA_GC
Cuts at: 0 922 1353
Size: 922 431
Bme1580I G_kGCm'C
Cuts at: 0 225 672 1016 1353
Size: 225 447 344 337
Fragments arranged by size:
447 344 337 225
BmgBI CAC'GTC
Cuts at: 0 803 1353
Size: 803 550
BmrI ACTGGGnnnn_n'
Cuts at: 0 1014 1353
Size: 1014 339
BmtI G_CTAG'C
Cuts at: 0 1228 1353
Size: 1228 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 442 552 952 1069 1353
Size: 28 414 110 400 117 284
Fragments arranged by size:
414 400 284 117 110 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 1049 1353
Size: 1049 304
BsaAI yAC'GTr
Cuts at: 0 842 1212 1353
Size: 842 370 141
Fragments arranged by size:
842 370 141
BsaHI Gr'CG_yC
Cuts at: 0 200 848 1353
Size: 200 648 505
Fragments arranged by size:
648 505 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1111 1141 1353
Size: 1111 30 212
Fragments arranged by size:
1111 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 208 1180 1353
Size: 148 60 972 173
Fragments arranged by size:
972 173 148 60
BseYI C'CCAG_C
Cuts at: 0 483 488 621 1353
Size: 483 5 133 732
Fragments arranged by size:
732 483 133 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1353
Size: 739 614
BsiEI CG_ry'CG
Cuts at: 0 21 525 1035 1125 1353
Size: 21 504 510 90 228
Fragments arranged by size:
510 504 228 90 21
Bsp1286I G_dGCh'C
Cuts at: 0 225 672 971 1016 1353
Size: 225 447 299 45 337
Fragments arranged by size:
447 337 299 225 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 1072 1353
Size: 483 589 281
Fragments arranged by size:
589 483 281
BsrBI CCG'CTC
Cuts at: 0 336 1127 1353
Size: 336 791 226
Fragments arranged by size:
791 336 226
BsrDI GCAATG_nn'
Cuts at: 0 318 1353
Size: 318 1035
BsrFI r'CCGG_y
Cuts at: 0 749 1034 1353
Size: 749 285 319
Fragments arranged by size:
749 319 285
BsrGI T'GTAC_A
Cuts at: 0 371 1353
Size: 371 982
BssSI C'ACGA_G
Cuts at: 0 784 1353
Size: 784 569
BstXI CCAn_nnnn'nTGG
Cuts at: 0 858 1047 1353
Size: 858 189 306
Fragments arranged by size:
858 306 189
BstYI r'GATC_y
Cuts at: 0 824 998 1324 1353
Size: 824 174 326 29
Fragments arranged by size:
824 326 174 29
Bsu36I CC'TnA_GG
Cuts at: 0 386 1273 1353
Size: 386 887 80
Fragments arranged by size:
887 386 80
BtgI C'CryG_G
Cuts at: 0 600 831 1119 1353
Size: 600 231 288 234
Fragments arranged by size:
600 288 234 231
DraI TTT'AAA
Cuts at: 0 231 1353
Size: 231 1122
EaeI y'GGCC_r
Cuts at: 0 18 603 894 1122 1353
Size: 18 585 291 228 231
Fragments arranged by size:
585 291 231 228 18
EagI C'GGCC_G
Cuts at: 0 18 1122 1353
Size: 18 1104 231
Fragments arranged by size:
1104 231 18
EarI CTCTTCn'nnn_
Cuts at: 0 133 214 1353
Size: 133 81 1139
Fragments arranged by size:
1139 133 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 61 1321 1353
Size: 41 20 1260 32
Fragments arranged by size:
1260 41 32 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 442 552 952 1069 1353
Size: 28 414 110 400 117 284
Fragments arranged by size:
414 400 284 117 110 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 787 862 1353
Size: 787 75 491
Fragments arranged by size:
787 491 75
EcoO109I rG'GnC_Cy
Cuts at: 0 426 867 1013 1353
Size: 426 441 146 340
Fragments arranged by size:
441 426 340 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 1353
Size: 162 1191
HaeII r_GCGC'y
Cuts at: 0 1024 1353
Size: 1024 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 1353
Size: 236 32 1085
Fragments arranged by size:
1085 236 32
MscI TGG'CCA
Cuts at: 0 896 1353
Size: 896 457
MslI CAynn'nnrTG
Cuts at: 0 842 1353
Size: 842 511
MspA1I CmG'CkG
Cuts at: 0 101 345 602 1353
Size: 101 244 257 751
Fragments arranged by size:
751 257 244 101
NaeI GCC'GGC
Cuts at: 0 751 1353
Size: 751 602
NcoI C'CATG_G
Cuts at: 0 831 1353
Size: 831 522
NgoMIV G'CCGG_C
Cuts at: 0 749 1353
Size: 749 604
NheI G'CTAG_C
Cuts at: 0 1224 1353
Size: 1224 129
PfoI T'CCnGG_A
Cuts at: 0 545 690 1353
Size: 545 145 663
Fragments arranged by size:
663 545 145
PpuMI rG'GwC_Cy
Cuts at: 0 426 867 1353
Size: 426 441 486
Fragments arranged by size:
486 441 426
PspOMI G'GGCC_C
Cuts at: 0 1012 1353
Size: 1012 341
PvuII CAG'CTG
Cuts at: 0 345 1353
Size: 345 1008
SacII CC_GC'GG
Cuts at: 0 603 1353
Size: 603 750
SapI GCTCTTCn'nnn_
Cuts at: 0 133 214 1353
Size: 133 81 1139
Fragments arranged by size:
1139 133 81
SexAI A'CCwGG_T
Cuts at: 0 863 1353
Size: 863 490
SmaI CCC'GGG
Cuts at: 0 114 1353
Size: 114 1239
SnaBI TAC'GTA
Cuts at: 0 842 1353
Size: 842 511
StyI C'CwwG_G
Cuts at: 0 731 831 1353
Size: 731 100 522
Fragments arranged by size:
731 522 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 539 1353
Size: 539 814
TatI w'GTAC_w
Cuts at: 0 371 767 1353
Size: 371 396 586
Fragments arranged by size:
586 396 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1353
Size: 1111 32 210
Fragments arranged by size:
1111 210 32
Tth111I GACn'n_nGTC
Cuts at: 0 541 801 865 1353
Size: 541 260 64 488
Fragments arranged by size:
541 488 260 64
XmaI C'CCGG_G
Cuts at: 0 112 1353
Size: 112 1241
Enzymes that do cut:
AlfI AlwNI ApaI AvaI BanII BbsI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I
BtgI DraI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NheI PfoI PpuMI PspOMI PvuII SacII
SapI SexAI SmaI SnaBI StyI TaqII TatI TstI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BplI Bpu10I BpuEI BsaBI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmlI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
XmnI ZraI