Restriction map for
pFN21AB4800
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
AspI BamHI BbsI BbvCI BciVI
BclI BfrI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 838 GGTACC G^GTACC
AlwNI 231 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 428 GGGCCC GGGCC^C
Asp718I 838 GGTACC G^GTACC
AviII 821 TGCGCA TGC^GCA
AvrII 588 CCTAGG C^CTAGG
BbrPI 817 CACGTG CAC^GTG
BcgI 869 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 589 CTAG C^TAG
BglI 798 GCCGCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 944 AGATCT A^GATCT
BlnI 588 CCTAGG C^CTAGG
BsaAI 817 CACGTG [CT]AC^GT[GA]
BsmI 1126 GCATTC GAATGC(1/-1)
BsrBI 372 GAGCGG CCGCTC(-3/-3)
BsrI 1087 ACTGG ACTGG(1/-1)
BstYI 944 AGATCT [GA]^GATC[CT]
DdeI 1002 CTCAG C^T[ACGT]AG
DpnII 945 GATC ^GATC
EagI 491 CGGCCG C^GGCCG
EclXI 491 CGGCCG C^GGCCG
Eco52I 491 CGGCCG C^GGCCG
EcoNI 583 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 588 CCTAGG C^C[AT][AT]GG
FspI 821 TGCGCA TGC^GCA
KpnI 838 GGTACC GGTAC^C
MaeI 589 CTAG C^TAG
MaeII 818 ACGT A^CGT
MaeIII 279 GTCAC ^GT[ACGT]AC
MboI 945 GATC ^GATC
MflI 944 AGATCT [GA]^GATC[CT]
MslI 1084 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 520 GCCGGC GCC^GGC
NdeII 945 GATC ^GATC
NgoMIV 520 GCCGGC G^CCGGC
PaeR7I 808 CTCGAG C^TCGAG
PleI 718 GAGTC GAGTC(4/5)
PmaCI 817 CACGTG CAC^GTG
PmlI 817 CACGTG CAC^GTG
PpuMI 826 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 552 CTGCAG CTGCA^G
SapI 954 GAAGAGC GCTCTTC(1/4)
Sau3AI 945 GATC ^GATC
ScaI 1028 AGTACT AGT^ACT
SexAI 401 ACCTGGT A^CC[AT]GGT
SfcI 552 CTGCAG C^T[GA][CT]AG
SmaI 152 CCCGGG CCC^GGG
SmlI 808 CTCGAG C^T[CT][GA]AG
StyI 588 CCTAGG C^C[AT][AT]GG
TaqI 809 TCGA T^CGA
TfiI 172 GAATC G^A[AT]TC
Tsp45I 279 GTCAC ^GT[GC]AC
TthHB8I 809 TCGA T^CGA
XhoI 808 CTCGAG C^TCGAG
XhoII 944 AGATCT [GA]^GATC[CT]
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 157 GACCGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
604 GACTTCGGGTC
Asp700I 954 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1206 GAAGGGTTTC
AspEI 157 GACCGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
604 GACTTCGGGTC
BalI 483 TGGCCA TGG^CCA
675 TGGCCA
BbeI 99 GGCGCC GGCGC^C
540 GGCGCC
BsiEI 418 CGACCG CG[GA][CT]^CG
491 CGGCCG
BsmAI 1246 GAGAC GTCTC(1/5)
291 GTCTC
BsmBI 290 CGTCTC CGTCTC(1/5)
1246 GAGACG
DraIII 400 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
883 CACGCGGTG
DsaI 641 CCACGG C^C[GA][CT]GG
678 CCACGG
Eam1105I 157 GACCGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
604 GACTTCGGGTC
EarI 457 CTCTTC CTCTTC(1/4)
954 GAAGAG
HinfI 172 GAATC G^A[ACGT]TC
718 GAGTC
KasI 99 GGCGCC G^GCGCC
540 GGCGCC
Ksp632I 457 CTCTTC CTCTTC(1/4)
954 GAAGAG
MluNI 483 TGGCCA TGG^CCA
675 TGGCCA
MscI 483 TGGCCA TGG^CCA
675 TGGCCA
NarI 99 GGCGCC GG^CGCC
540 GGCGCC
PvuII 178 CAGCTG CAG^CTG
219 CAGCTG
SacI 246 GAGCTC GAGCT^C
1137 GAGCTC
SfaNI 208 GCATC GCATC(5/9)
770 GCATC
SfoI 99 GGCGCC GGC^GCC
540 GGCGCC
TspRI 977 CACTG CA[GC]TG[ACGT][ACGT]^
1086 CACTG
XmnI 954 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1206 GAAGGGTTTC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I ApaLI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI TseI