(Circular) (Six-base) MAPSORT of: pFN21AB4800.seq Check: 3553 from: 1 to: 5971 pFN21AB4800 5971 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3003 3003 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 2843 3292 2843 Size: 449 5522 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4034 5418 5686 1632 Size: 2402 1384 268 1917 Fragments arranged by size: 2402 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 2167 2614 4700 2167 Size: 447 2086 3438 Fragments arranged by size: 3438 2086 447 AleI CACnn'nnGTG Cuts at: 1166 3093 1166 Size: 1927 4044 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2762 1184 Size: 1578 4393 AlwNI CAG_nnn'CTG Cuts at: 2241 3714 5286 2241 Size: 1473 1572 2926 Fragments arranged by size: 2926 1572 1473 ApaI G_GGCC'C Cuts at: 2437 2437 Size: 5971 ApaLI G'TGCA_C Cuts at: 2737 2819 2918 4028 5184 2737 Size: 82 99 1110 1156 3524 Fragments arranged by size: 3524 1156 1110 99 82 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2672 2813 3296 1870 Size: 73 214 515 141 483 4545 Fragments arranged by size: 4545 515 483 214 141 73 AvrII C'CTAG_G Cuts at: 2593 2593 Size: 5971 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2827 2860 2827 Size: 33 5938 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2403 2545 2573 2843 Size: 325 217 627 317 299 142 28 270 Cuts at: 2843 3243 3292 4747 618 Size: 400 49 1455 1842 Fragments arranged by size: 1842 1455 627 400 325 317 299 270 217 142 49 28 BanII G_rGCy'C Cuts at: 1391 2157 2255 2437 2601 2814 3146 3267 3290 Size: 766 98 182 164 213 332 121 23 Cuts at: 3290 1391 Size: 4072 Fragments arranged by size: 4072 766 332 213 182 164 121 98 23 BbeI G_GCGC'C Cuts at: 2108 2549 2108 Size: 441 5530 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2863 2897 4162 4196 1903 Size: 34 50 34 842 34 1265 34 3678 Fragments arranged by size: 3678 1265 842 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2809 4582 136 Size: 107 122 71 631 605 1137 1773 1525 Fragments arranged by size: 1773 1525 1137 631 605 122 107 71 BglII A'GATC_T Cuts at: 2949 5966 2949 Size: 3017 2954 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2324 2437 2741 2823 2889 2922 4032 5188 Size: 534 113 304 82 66 33 1110 1156 Cuts at: 5188 1790 Size: 2573 Fragments arranged by size: 2573 1156 1110 534 304 113 82 66 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3702 4660 442 Size: 416 53 83 399 24 2285 958 1753 Fragments arranged by size: 2285 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2293 2459 2921 4631 1239 Size: 1044 10 166 462 1710 2579 Fragments arranged by size: 2579 1710 1044 462 166 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 3762 493 Size: 711 1620 938 2702 Fragments arranged by size: 2702 1620 938 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 2546 2712 3778 Size: 53 83 186 936 572 441 166 1066 Cuts at: 3778 4160 4795 275 Size: 382 635 1451 Fragments arranged by size: 1451 1066 936 635 572 441 382 186 166 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2720 2750 1178 Size: 30 165 30 789 30 498 30 4399 Fragments arranged by size: 4399 789 498 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5971 BseYI C'CCAG_C Cuts at: 2340 2752 5174 5670 2340 Size: 412 2422 496 2641 Fragments arranged by size: 2641 2422 496 412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 2675 2803 1163 Size: 573 696 243 128 4331 Fragments arranged by size: 4331 696 573 243 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2426 2499 3421 4182 5210 1540 Size: 275 186 425 73 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 425 275 186 73 BsiHKAI G_wGCw'C Cuts at: 2255 2741 2823 2922 3146 4032 4117 5188 2255 Size: 486 82 99 224 886 85 1071 3038 Fragments arranged by size: 3038 1071 886 486 224 99 85 82 BsmI GAATG_Cn' Cuts at: 3131 3482 3575 3131 Size: 351 93 5527 Fragments arranged by size: 5527 351 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2301 3245 2301 Size: 944 5027 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2255 2324 2437 2601 2741 2814 Size: 399 367 98 69 113 164 140 73 Cuts at: 2814 2823 2889 2922 3146 3267 3290 4032 4117 Size: 9 66 33 224 121 23 742 85 Cuts at: 4117 5188 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 224 164 140 121 113 98 85 73 69 66 33 23 9 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 2820 3003 3326 844 Size: 1562 414 183 323 3489 Fragments arranged by size: 3489 1562 414 323 183 BsrBI CCG'CTC Cuts at: 1394 1823 2379 3858 1394 Size: 429 556 1479 3507 Fragments arranged by size: 3507 1479 556 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2525 2570 4615 4802 1700 Size: 110 291 424 45 2045 187 2869 Fragments arranged by size: 2869 2045 424 291 187 110 45 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 2241 2410 2781 794 Size: 1447 169 371 3984 Fragments arranged by size: 3984 1447 371 169 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2949 3301 3668 4053 4070 5522 5608 5620 Size: 977 352 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 977 385 367 352 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2646 2683 513 Size: 552 1581 37 3801 Fragments arranged by size: 3801 1581 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2310 487 Size: 683 56 1084 4148 Fragments arranged by size: 4148 1084 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DraIII CAC_nnn'GTG Cuts at: 2410 2893 2410 Size: 483 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2488 2496 2680 Size: 54 1425 50 275 18 658 8 184 Cuts at: 2680 3418 4307 5936 8 Size: 738 889 1629 43 Fragments arranged by size: 1629 1425 889 738 658 275 184 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2496 3418 1537 Size: 275 684 922 4090 Fragments arranged by size: 4090 922 684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2468 2954 3901 1353 Size: 1115 486 947 3423 Fragments arranged by size: 3423 1115 947 486 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 2165 4558 4932 5078 387 Size: 1064 138 570 6 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 570 374 146 138 6 EcoICRI GAG'CTC Cuts at: 2253 3144 2253 Size: 891 5080 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2293 2459 2921 4034 4631 5418 Size: 393 651 10 166 462 1113 597 787 Cuts at: 5418 5686 1239 Size: 268 1524 Fragments arranged by size: 1524 1113 787 651 597 462 393 268 166 10 EcoNI CCTnn'n_nnAGG Cuts at: 2592 2592 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2277 2433 2434 2832 2277 Size: 156 1 398 5416 Fragments arranged by size: 5416 398 156 1 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 2828 4477 2828 Size: 1649 4322 HaeII r_GCGC'y Cuts at: 1678 2108 2451 2549 2709 5118 1678 Size: 430 343 98 160 2409 2531 Fragments arranged by size: 2531 2409 430 343 160 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2192 2224 2600 Size: 32 135 32 581 32 7 32 376 Cuts at: 2600 2632 4612 4644 4686 4718 1373 Size: 32 1980 32 42 32 2626 Fragments arranged by size: 2626 1980 581 376 135 42 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KasI G'GCGC_C Cuts at: 2104 2545 2104 Size: 441 5530 KpnI G_GTAC'C Cuts at: 2847 3296 2847 Size: 449 5522 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 3171 5085 5269 1305 Size: 1401 465 1914 184 2007 Fragments arranged by size: 2007 1914 1401 465 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 2682 10 Size: 54 1425 343 658 192 3299 Fragments arranged by size: 3299 1425 658 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 3093 3929 4288 4447 518 Size: 648 1927 836 359 159 2042 Fragments arranged by size: 2042 1927 836 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2226 2582 2654 2841 3387 4064 5212 Size: 579 41 356 72 187 546 677 1148 Cuts at: 5212 5457 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 579 546 356 245 187 72 41 NaeI GCC'GGC Cuts at: 2527 2527 Size: 5971 NarI GG'CG_CC Cuts at: 2105 2546 2105 Size: 441 5530 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NgoMIV G'CCGG_C Cuts at: 2525 2525 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PmlI CAC'GTG Cuts at: 2824 2824 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2832 2832 Size: 5971 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2433 2433 Size: 5971 PspXI vC'TCGA_Gb Cuts at: 2813 2813 Size: 5971 PstI C_TGCA'G Cuts at: 830 2561 3323 830 Size: 1731 762 3478 Fragments arranged by size: 3478 1731 762 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2185 2226 2185 Size: 41 5930 SacI G_AGCT'C Cuts at: 2255 3146 2255 Size: 891 5080 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2954 2954 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 3035 4219 5931 3035 Size: 1184 1712 3075 Fragments arranged by size: 3075 1712 1184 SexAI A'CCwGG_T Cuts at: 2406 2406 Size: 5971 SfcI C'TryA_G Cuts at: 826 1045 2557 3319 4454 5135 5326 826 Size: 219 1512 762 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 762 681 219 191 SfoI GGC'GCC Cuts at: 2106 2547 2106 Size: 441 5530 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 2159 3298 1872 Size: 287 1139 4545 Fragments arranged by size: 4545 1139 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 2813 4075 4976 5238 820 Size: 197 926 870 1262 901 262 1553 Fragments arranged by size: 1553 1262 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 2593 3410 513 Size: 552 1528 817 3074 Fragments arranged by size: 3074 1528 817 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 3173 3998 4015 4168 4353 4798 Size: 1255 624 969 825 17 153 185 445 Cuts at: 4798 325 Size: 1498 Fragments arranged by size: 1498 1255 969 825 624 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 4217 5929 Size: 274 80 33 51 1443 1056 1184 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1712 1443 1184 1056 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2720 2752 5931 5963 2720 Size: 32 3179 32 2728 Fragments arranged by size: 3179 2728 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 2813 1943 Size: 870 5101 XmaI C'CCGG_G Cuts at: 1870 2157 3296 1870 Size: 287 1139 4545 Fragments arranged by size: 4545 1139 287 XmnI GAAnn'nnTTC Cuts at: 2963 3215 4100 2963 Size: 252 885 4834 Fragments arranged by size: 4834 885 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI