(Circular) (Six-base) MAPSORT of: pFN21AB4783.seq Check: 5157 from: 1 to: 6661 pFN21AB4783 6661 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2542 2542 Size: 6661 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2680 4471 278 Size: 53 83 186 936 1144 1791 2468 Fragments arranged by size: 2468 1791 1144 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4004 1937 Size: 2067 4594 Acc65I G'GTAC_C Cuts at: 3314 3982 3314 Size: 668 5993 AclI AA'CG_TT Cuts at: 1563 4788 5161 1563 Size: 3225 373 3063 Fragments arranged by size: 3225 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3471 4724 6108 6376 1632 Size: 1839 1253 1384 268 1917 Fragments arranged by size: 1917 1839 1384 1253 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 5560 6537 1203 Size: 4357 977 1327 Fragments arranged by size: 4357 1327 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 5390 5390 Size: 6661 AleI CACnn'nnGTG Cuts at: 1166 2827 1166 Size: 1661 5000 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6661 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2766 2798 2766 Size: 32 6629 AlwNI CAG_nnn'CTG Cuts at: 2824 3847 4404 5976 2824 Size: 1023 557 1572 3509 Fragments arranged by size: 3509 1572 1023 557 ApaLI G'TGCA_C Cuts at: 3275 4718 5874 3275 Size: 1443 1156 4062 Fragments arranged by size: 4062 1443 1156 ApoI r'AATT_y Cuts at: 1493 2093 2164 3640 3970 4196 4348 6457 1493 Size: 600 71 1476 330 226 152 2109 1697 Fragments arranged by size: 2109 1697 1476 600 330 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 3986 1870 Size: 73 2043 4545 Fragments arranged by size: 4545 2043 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3298 3331 3298 Size: 33 6628 BamHI G'GATC_C Cuts at: 3991 4358 3991 Size: 367 6294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 3314 3686 3710 3982 Size: 325 217 627 1385 142 372 24 272 Cuts at: 3982 5437 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1385 627 372 325 272 217 142 24 BanII G_rGCy'C Cuts at: 1391 2148 3797 3980 1391 Size: 757 1649 183 4072 Fragments arranged by size: 4072 1649 757 183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2435 928 Size: 971 536 5154 Fragments arranged by size: 5154 971 536 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4852 4886 1903 Size: 34 50 34 2831 34 3678 Fragments arranged by size: 3678 2831 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3119 4553 5763 3119 Size: 1434 1210 4017 Fragments arranged by size: 4017 1434 1210 BclI T'GATC_A Cuts at: 1548 3237 1548 Size: 1689 4972 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2815 3392 5272 Size: 107 122 71 631 605 1143 577 1880 Cuts at: 5272 136 Size: 1525 Fragments arranged by size: 1880 1525 1143 631 605 577 122 107 71 BglII A'GATC_T Cuts at: 2655 6656 2655 Size: 4001 2660 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 3279 4722 5878 1790 Size: 1489 1443 1156 2573 Fragments arranged by size: 2573 1489 1443 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3786 4392 5350 Size: 416 53 83 399 24 2369 606 958 Cuts at: 5350 442 Size: 1753 Fragments arranged by size: 2369 1753 958 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 2924 2933 5321 1239 Size: 1475 210 9 2388 2579 Fragments arranged by size: 2579 2388 1475 210 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3714 4786 5651 5949 3714 Size: 1072 865 298 4426 Fragments arranged by size: 4426 1072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5324 882 Size: 617 3825 2219 Fragments arranged by size: 3825 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3956 4452 493 Size: 711 2752 496 2702 Fragments arranged by size: 2752 2702 711 496 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2677 4468 4850 5485 Size: 53 83 186 936 1144 1791 382 635 Cuts at: 5485 275 Size: 1451 Fragments arranged by size: 1791 1451 1144 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2182 3474 5094 5492 5766 5913 6324 2182 Size: 1292 1620 398 274 147 411 2519 Fragments arranged by size: 2519 1620 1292 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6436 Fragments arranged by size: 6436 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 3447 3555 3918 3921 3924 3933 3936 Size: 842 1419 108 363 3 3 9 3 Cuts at: 3936 1186 Size: 3911 Fragments arranged by size: 3911 1419 842 363 108 9 3 3 3 BseYI C'CCAG_C Cuts at: 2538 5864 6360 2538 Size: 3326 496 2839 Fragments arranged by size: 3326 2839 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6088 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2614 3279 3658 3797 4722 4807 5878 2148 Size: 466 665 379 139 925 85 1071 2931 Fragments arranged by size: 2931 1071 925 665 466 379 139 85 BsmI GAATG_Cn' Cuts at: 2760 4172 4265 2760 Size: 1412 93 5156 Fragments arranged by size: 5156 1412 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3046 3046 Size: 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2614 3279 3658 3797 3980 4722 Size: 399 358 466 665 379 139 183 742 Cuts at: 4722 4807 5878 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 665 466 399 379 358 183 139 85 BspEI T'CCGG_A Cuts at: 2182 6324 2182 Size: 4142 2519 BspHI T'CATG_A Cuts at: 4550 6280 4550 Size: 1730 4931 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 4016 844 Size: 1698 1474 3489 Fragments arranged by size: 3489 1698 1474 BsrBI CCG'CTC Cuts at: 1394 1823 4548 1394 Size: 429 2725 3507 Fragments arranged by size: 3507 2725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 5156 5330 65 Size: 1183 1158 2750 174 1396 Fragments arranged by size: 2750 1396 1183 1158 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3822 5305 5492 1700 Size: 110 2012 1483 187 2869 Fragments arranged by size: 2869 2012 1483 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6661 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 4721 5733 4721 Size: 1012 5649 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6661 BstBI TT'CG_AA Cuts at: 5465 5465 Size: 6661 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6661 BstYI r'GATC_y Cuts at: 1972 2655 2781 3991 4358 4743 4760 6212 6298 Size: 683 126 1210 367 385 17 1452 86 Cuts at: 6298 6310 6656 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1210 683 385 367 346 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2047 3112 3205 513 Size: 552 982 1065 93 3969 Fragments arranged by size: 3969 1065 982 552 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 3628 4177 4971 4991 3628 Size: 549 794 20 5298 Fragments arranged by size: 5298 794 549 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3535 3570 529 Size: 35 2971 35 3620 Fragments arranged by size: 3620 2971 35 35 DraI TTT'AAA Cuts at: 3965 3965 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2556 2843 Size: 54 1425 50 275 18 559 167 287 Cuts at: 2843 3095 3286 3556 4108 4997 6626 8 Size: 252 191 270 552 889 1629 43 Fragments arranged by size: 1629 1425 889 559 552 287 275 270 252 191 167 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 3375 4591 1353 Size: 1229 793 1216 3423 Fragments arranged by size: 3423 1229 1216 793 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3456 5248 5622 5768 387 Size: 1064 138 1867 1792 374 146 1280 Fragments arranged by size: 1867 1792 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 3795 2146 Size: 1649 5012 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2714 2924 2933 3471 4724 5321 6108 Size: 393 1082 210 9 538 1253 597 787 Cuts at: 6108 6376 1239 Size: 268 1524 Fragments arranged by size: 1524 1253 1082 787 597 538 393 268 210 9 EcoNI CCTnn'n_nnAGG Cuts at: 3350 3350 Size: 6661 EcoO109I rG'GnC_Cy Cuts at: 2621 2621 Size: 6661 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 3083 5167 3083 Size: 2084 4577 HaeII r_GCGC'y Cuts at: 1678 5808 1678 Size: 4130 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3185 3217 3351 3383 5302 Size: 32 135 32 1613 32 134 32 1919 Cuts at: 5302 5334 5376 5408 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1919 1613 135 134 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 3128 4005 4251 669 Size: 988 281 859 331 877 246 3079 Fragments arranged by size: 3079 988 877 859 331 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3318 3986 3318 Size: 668 5993 MfeI C'AATT_G Cuts at: 2454 4260 2454 Size: 1806 4855 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3013 5775 5959 1305 Size: 1708 2762 184 2007 Fragments arranged by size: 2762 2007 1708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2845 3097 10 Size: 54 1425 343 1013 252 3574 Fragments arranged by size: 3574 1425 1013 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2827 4619 4978 5137 518 Size: 648 1661 1792 359 159 2042 Fragments arranged by size: 2042 1792 1661 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3701 4077 4754 5902 6147 1606 Size: 2095 376 677 1148 245 2120 Fragments arranged by size: 2120 2095 1148 677 376 245 NcoI C'CATG_G Cuts at: 513 1065 3112 513 Size: 552 2047 4062 Fragments arranged by size: 4062 2047 552 NdeI CA'TA_TG Cuts at: 387 2206 387 Size: 1819 4842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 4019 5564 1132 Size: 97 2790 1545 2229 Fragments arranged by size: 2790 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3338 1348 Size: 1990 4671 PciI A'CATG_T Cuts at: 5560 5560 Size: 6661 PflMI CCAn_nnn'nTGG Cuts at: 1108 2947 2995 1108 Size: 1839 48 4774 Fragments arranged by size: 4774 1839 48 PfoI T'CCnGG_A Cuts at: 2671 2691 3874 2671 Size: 20 1183 5458 Fragments arranged by size: 5458 1183 20 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2621 2621 Size: 6661 PshAI GACnn'nnGTC Cuts at: 1613 3123 1613 Size: 1510 5151 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PstI C_TGCA'G Cuts at: 830 4013 830 Size: 3183 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 PvuII CAG'CTG Cuts at: 3701 3701 Size: 6661 SacI G_AGCT'C Cuts at: 2148 3797 2148 Size: 1649 5012 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SbfI CC_TGCA'GG Cuts at: 4013 4013 Size: 6661 ScaI AGT'ACT Cuts at: 2593 4909 6621 2593 Size: 2316 1712 2633 Fragments arranged by size: 2633 2316 1712 SfcI C'TryA_G Cuts at: 826 1045 3189 4009 5144 5825 6016 826 Size: 219 2144 820 1135 681 191 1471 Fragments arranged by size: 2144 1471 1135 820 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 3988 1872 Size: 2116 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3729 4765 5666 5928 820 Size: 197 926 1786 1036 901 262 1553 Fragments arranged by size: 1786 1553 1036 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 4019 1132 Size: 2887 3774 SspI AAT'ATT Cuts at: 5 52 3036 4585 5 Size: 47 2984 1549 2081 Fragments arranged by size: 2984 2081 1549 47 StuI AGG'CCT Cuts at: 1517 2809 1517 Size: 1292 5369 StyI C'CwwG_G Cuts at: 513 1065 3112 4100 513 Size: 552 2047 988 3074 Fragments arranged by size: 3074 2047 988 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2906 3190 3543 4688 4705 4858 5043 Size: 1255 1326 284 353 1145 17 153 185 Cuts at: 5043 5488 325 Size: 445 1498 Fragments arranged by size: 1498 1326 1255 1145 445 353 284 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2545 2591 2723 Size: 274 80 33 51 1443 568 46 132 Cuts at: 2723 4907 6619 96 Size: 2184 1712 138 Fragments arranged by size: 2184 1712 1443 568 274 138 132 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6621 6653 6621 Size: 32 6629 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 2579 3997 2579 Size: 1418 5243 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3138 3674 3809 3138 Size: 536 135 5990 Fragments arranged by size: 5990 536 135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 3986 1870 Size: 2116 4545 XmnI GAAnn'nnTTC Cuts at: 4790 4790 Size: 6661 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2678 4469 276 Size: 53 83 186 936 1144 1791 2468 Fragments arranged by size: 2468 1791 1144 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI