(Circular) (Six-base) MAPSORT of: pFN21AB4718.seq Check: 8296 from: 1 to: 5338 pFN21AB4718 5338 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3148 278 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 2681 1937 Size: 250 494 4594 Fragments arranged by size: 4594 494 250 Acc65I G'GTAC_C Cuts at: 2586 2659 2586 Size: 73 5265 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3401 4785 5053 1632 Size: 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2282 820 Size: 197 1265 3876 Fragments arranged by size: 3876 1265 197 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 2311 3081 4653 2311 Size: 770 1572 2996 Fragments arranged by size: 2996 1572 770 ApaI G_GGCC'C Cuts at: 2172 2602 2172 Size: 430 4908 ApaLI G'TGCA_C Cuts at: 2074 3395 4551 2074 Size: 1321 1156 2861 Fragments arranged by size: 2861 1321 1156 ApoI r'AATT_y Cuts at: 1493 2647 2873 3025 5134 1493 Size: 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1154 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2663 1870 Size: 73 75 645 4545 Fragments arranged by size: 4545 645 75 73 BamHI G'GATC_C Cuts at: 2668 3035 2668 Size: 367 4971 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 2586 2659 4114 618 Size: 325 217 627 276 523 73 1455 1842 Fragments arranged by size: 1842 1455 627 523 325 276 217 73 BanII G_rGCy'C Cuts at: 1391 2025 2172 2602 2657 1391 Size: 634 147 430 55 4072 Fragments arranged by size: 4072 634 430 147 55 BbeI G_GCGC'C Cuts at: 2067 2067 Size: 5338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3949 136 Size: 107 122 71 631 605 2277 1525 Fragments arranged by size: 2277 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2078 2172 2602 3399 4555 1790 Size: 288 94 430 797 1156 2573 Fragments arranged by size: 2573 1156 797 430 288 94 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3069 4027 Size: 416 53 83 399 24 651 1001 958 Cuts at: 4027 442 Size: 1753 Fragments arranged by size: 1753 1001 958 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2309 3998 1239 Size: 826 244 1689 2579 Fragments arranged by size: 2579 1689 826 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3129 493 Size: 711 1925 2702 Fragments arranged by size: 2702 1925 711 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2103 3145 3527 Size: 53 83 186 936 531 39 1042 382 Cuts at: 3527 4162 275 Size: 635 1451 Fragments arranged by size: 1451 1042 936 635 531 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 1178 Size: 30 165 30 1092 30 3991 Fragments arranged by size: 3991 1092 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5338 BseYI C'CCAG_C Cuts at: 2087 2394 4541 5037 2087 Size: 307 2147 496 2388 Fragments arranged by size: 2388 2147 496 307 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 1163 Size: 573 525 4240 Fragments arranged by size: 4240 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2788 3549 4577 1540 Size: 275 186 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2078 3399 3484 4555 2034 Size: 44 1321 85 1071 2817 Fragments arranged by size: 2817 1321 1071 85 44 BsmI GAATG_Cn' Cuts at: 2849 2942 2849 Size: 93 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2034 2078 2172 2602 2657 3399 Size: 399 235 9 44 94 430 55 742 Cuts at: 3399 3484 4555 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 235 94 85 55 44 9 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2469 2693 844 Size: 1475 150 224 3489 Fragments arranged by size: 3489 1475 224 150 BsrBI CCG'CTC Cuts at: 1394 1823 3225 1394 Size: 429 1402 3507 Fragments arranged by size: 3507 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3982 4169 1700 Size: 110 2172 187 2869 Fragments arranged by size: 2869 2172 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5338 BssSI C'ACGA_G Cuts at: 3398 4410 3398 Size: 1012 4326 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 2522 1184 Size: 1338 4000 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2143 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333 Size: 696 367 385 17 1452 86 12 346 Cuts at: 5333 1972 Size: 1977 Fragments arranged by size: 1977 1452 696 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4786 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2854 3648 3668 2854 Size: 794 20 4524 Fragments arranged by size: 4524 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2785 3674 5303 Size: 54 1425 50 275 18 955 889 1629 Cuts at: 5303 8 Size: 43 Fragments arranged by size: 1629 1425 955 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2785 1537 Size: 275 973 4090 Fragments arranged by size: 4090 973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3268 1353 Size: 1915 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3925 4299 4445 387 Size: 1064 138 2336 374 146 1280 Fragments arranged by size: 2336 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2309 3401 3998 4785 5053 1239 Size: 393 433 244 1092 597 787 268 1524 Fragments arranged by size: 1524 1092 787 597 433 393 268 244 EcoO109I rG'GnC_Cy Cuts at: 2168 2169 2599 2168 Size: 1 430 4907 Fragments arranged by size: 4907 430 1 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 2067 4485 1678 Size: 389 2418 2531 Fragments arranged by size: 2531 2418 389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 3979 4011 4053 Size: 32 135 32 923 32 1452 32 42 Cuts at: 4053 4085 1373 Size: 32 2626 Fragments arranged by size: 2626 1452 923 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 2682 2928 669 Size: 988 281 358 386 246 3079 Fragments arranged by size: 3079 988 386 358 281 246 HindIII A'AGCT_T Cuts at: 748 2259 748 Size: 1511 3827 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KasI G'GCGC_C Cuts at: 2063 2063 Size: 5338 KpnI G_GTAC'C Cuts at: 2590 2663 2590 Size: 73 5265 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 4452 4636 1305 Size: 837 2310 184 2007 Fragments arranged by size: 2310 2007 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3516 Fragments arranged by size: 3516 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 3296 3655 3814 518 Size: 648 1062 1068 359 159 2042 Fragments arranged by size: 2042 1068 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2583 2754 3431 4579 4824 1606 Size: 437 540 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 437 245 171 NarI GG'CG_CC Cuts at: 2064 2064 Size: 5338 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4786 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2082 2509 2696 4241 1132 Size: 97 853 427 187 1545 2229 Fragments arranged by size: 2229 1545 853 427 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5338 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PspOMI G'GGCC_C Cuts at: 2168 2598 2168 Size: 430 4908 PstI C_TGCA'G Cuts at: 830 2582 2690 830 Size: 1752 108 3478 Fragments arranged by size: 3478 1752 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2043 2583 2043 Size: 540 4798 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2578 2686 3821 4502 4693 826 Size: 219 1533 108 1135 681 191 1471 Fragments arranged by size: 1533 1471 1135 681 219 191 108 SfoI GGC'GCC Cuts at: 2065 2065 Size: 5338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 3442 4343 4605 820 Size: 197 926 339 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 339 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2012 2136 2777 513 Size: 552 947 124 641 3074 Fragments arranged by size: 3074 947 641 552 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2516 3365 3382 3535 3720 4165 325 Size: 1255 936 849 17 153 185 445 1498 Fragments arranged by size: 1498 1255 936 849 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2343 3584 Size: 274 80 33 51 1443 267 99 1241 Cuts at: 3584 5296 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1241 274 267 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3146 276 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI