Restriction map for
pFN21AB4667
(Circular) (Six-base) MAPSORT of: pFN21AB4667.seq Check: 4342 from: 1 to: 5770
pFN21AB4667 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2079 2987 2079
Size: 908 4862
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3033 3580 278
Size: 53 83 186 936 1497 547 2468
Fragments arranged by size:
2468 1497 936 547 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2910 3113 1937
Size: 973 203 4594
Fragments arranged by size:
4594 973 203
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2410 3833 5217 5485 1632
Size: 778 1423 1384 268 1917
Fragments arranged by size:
1917 1423 1384 778 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2889 4669 5646 1203
Size: 1686 1780 977 1327
Fragments arranged by size:
1780 1686 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2343 2437 1184
Size: 1159 94 4517
Fragments arranged by size:
4517 1159 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 5738
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaI G_GGCC'C
Cuts at: 2266 2342 2266
Size: 76 5694
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 2538 2870 3095 1870
Size: 73 426 169 332 225 4545
Fragments arranged by size:
4545 426 332 225 169 73
BamHI G'GATC_C
Cuts at: 2640 3100 3467 2640
Size: 460 367 4943
Fragments arranged by size:
4943 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 3091 4546 618
Size: 325 217 627 336 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 336 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2342 2589 2688 3089 1391
Size: 875 76 247 99 401 4072
Fragments arranged by size:
4072 875 401 247 99 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2937 3004 928
Size: 971 1038 67 3694
Fragments arranged by size:
3694 1038 971 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2961 5765 2961
Size: 2804 2966
BlpI GC'TnA_GC
Cuts at: 2687 3187 2687
Size: 500 5270
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2266 2342 2416 3831 4987 1790
Size: 336 140 76 74 1415 1156 2573
Fragments arranged by size:
2573 1415 1156 336 140 76 74
BmgBI CAC'GTC
Cuts at: 1318 2608 1318
Size: 1290 4480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2419 2471 2504 4430 1239
Size: 974 206 52 33 1926 2579
Fragments arranged by size:
2579 1926 974 206 52 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3895 4760 5058 3035
Size: 860 865 298 3747
Fragments arranged by size:
3747 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2945 4433 882
Size: 617 1446 1488 2219
Fragments arranged by size:
2219 1488 1446 617
BsaAI yAC'GTr
Cuts at: 493 1204 2892 3561 493
Size: 711 1688 669 2702
Fragments arranged by size:
2702 1688 711 669
BsaBI GATnn'nnATC
Cuts at: 2165 5764 2165
Size: 3599 2171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 3030 3577 3959
Size: 53 83 186 936 1125 372 547 382
Cuts at: 3959 4594 275
Size: 635 1451
Fragments arranged by size:
1451 1125 936 635 547 382 372 186
83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 1178
Size: 30 165 30 1152 30 4363
Fragments arranged by size:
4363 1152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2912 1186
Size: 1726 4044
BseYI C'CCAG_C
Cuts at: 2119 2377 4973 5469 2119
Size: 258 2596 496 2420
Fragments arranged by size:
2596 2420 496 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4594
Fragments arranged by size:
4594 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2302 3220 3981 5009 1540
Size: 275 186 301 918 761 1028 2301
Fragments arranged by size:
2301 1028 918 761 301 275 186
BsiHKAI G_wGCw'C
Cuts at: 2147 2589 2688 3831 3916 4987 2147
Size: 442 99 1143 85 1071 2930
Fragments arranged by size:
2930 1143 1071 442 99 85
BsiWI C'GTAC_G
Cuts at: 2892 2892
Size: 5770
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2147 2266 2342 2416 2589 2688
Size: 399 336 21 119 76 74 173 99
Cuts at: 2688 3089 3831 3916 4987 1391
Size: 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 336 173 119
99 85 76 74 21
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2832 2987 3125 844
Size: 1235 753 155 138 3489
Fragments arranged by size:
3489 1235 753 155 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2433 2854 3657 1394
Size: 429 610 421 803 3507
Fragments arranged by size:
3507 803 610 429 421
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2640 2838 2961 3100 3467 3852 3869 5321
Size: 668 198 123 139 367 385 17 1452
Cuts at: 5321 5407 5419 5765 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 198 139
123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2276 2276
Size: 5770
BtgI C'CryG_G
Cuts at: 513 1065 2004 2550 513
Size: 552 939 546 3733
Fragments arranged by size:
3733 939 552 546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2476 487
Size: 683 56 1250 3781
Fragments arranged by size:
3781 1250 683 56
BtsI GCAGTG_nn'
Cuts at: 2644 3286 4080 4100 2644
Size: 642 794 20 4314
Fragments arranged by size:
4314 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2539 2574 529
Size: 35 1975 35 3725
Fragments arranged by size:
3725 1975 35 35
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 3217 4106
Size: 54 1425 50 275 18 438 949 889
Cuts at: 4106 5735 8
Size: 1629 43
Fragments arranged by size:
1629 1425 949 889 438 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 3700 1353
Size: 1457 890 3423
Fragments arranged by size:
3423 1457 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2831 4357 4731 4877 387
Size: 1064 138 1242 1526 374 146 1280
Fragments arranged by size:
1526 1280 1242 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2587 2686 2587
Size: 99 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2410 2419 2471 2504 3833 4430
Size: 393 581 197 9 52 33 1329 597
Cuts at: 4430 5217 5485 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1329 787 597 581 393 268 197
52 33 9
EcoO109I rG'GnC_Cy
Cuts at: 2263 2339 2821 2263
Size: 76 482 5212
Fragments arranged by size:
5212 482 76
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373
Size: 32 135 32 2839 32 42 32 2626
Fragments arranged by size:
2839 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2711 4884 5068 1305
Size: 1406 2173 184 2007
Fragments arranged by size:
2173 2007 1406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 4087 4246 518
Size: 648 2562 359 159 2042
Fragments arranged by size:
2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2178 2345 2501 2691 2906 2920 3186
Size: 483 89 167 156 190 215 14 266
Cuts at: 3186 3863 5011 5256 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 483 266 245 215 190
167 156 89 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2408 1348
Size: 1060 4710
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2051 2051
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 3839 3871 5352 5384 2555
Size: 32 1252 32 1481 32 2941
Fragments arranged by size:
2941 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 2821
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 3029 1613
Size: 1416 4354
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PspOMI G'GGCC_C
Cuts at: 2262 2338 2262
Size: 76 5694
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2501 2501
Size: 5770
SacI G_AGCT'C
Cuts at: 2589 2688 2589
Size: 99 5671
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 3118 4253 4934 5125 826
Size: 219 2073 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2540 3097 1872
Size: 668 557 4545
Fragments arranged by size:
4545 668 557
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3050 3874 4775 5037 820
Size: 197 926 1107 824 901 262 1553
Fragments arranged by size:
1553 1107 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 2198 2420 1517
Size: 681 222 4867
Fragments arranged by size:
4867 681 222
StyI C'CwwG_G
Cuts at: 513 1065 2004 2955 3209 513
Size: 552 939 951 254 3074
Fragments arranged by size:
3074 951 939 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 3797 3814 3967 4152 4597 325
Size: 1255 789 1428 17 153 185 445 1498
Fragments arranged by size:
1498 1428 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2874 3054
Size: 274 80 33 51 1443 231 666 180
Cuts at: 3054 4016 5728 96
Size: 962 1712 138
Fragments arranged by size:
1712 1443 962 666 274 231 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2557 2557
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2538 3095 1870
Size: 668 557 4545
Fragments arranged by size:
4545 668 557
XmnI GAAnn'nnTTC
Cuts at: 2570 3899 2570
Size: 1329 4441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3031 3578 276
Size: 53 83 186 936 1497 547 2468
Fragments arranged by size:
2468 1497 936 547 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI