Restriction map for
pFN21AB4637
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BseRI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MluNI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 187 GGTACC G^GTACC
AccIII 788 TCCGGA T^CCGGA
AcsI 88 AAATTC [GA]^AATT[CT]
AhdI 226 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 535 GGATC GGATC(4/5)
AlwNI 251 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 88 AAATTC [GA]^AATT[CT]
Asp700I 885 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 187 GGTACC G^GTACC
AspEI 226 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 187 GGTACC G^G[CT][GA]CC
BanII 876 GGGCTC G[GA]GC[CT]^C
BbsI 76 GAAGAC GAAGAC(2/6)
BclI 558 TGATCA T^GATCA
BpuAI 76 GAAGAC GAAGAC(2/6)
BsaAI 442 TACGTG [CT]AC^GT[GA]
BsaI 448 GAGACC GGTCTC(1/5)
BsaWI 788 TCCGGA [AT]^CCGG[AT]
BseAI 788 TCCGGA T^CCGGA
BsgI 93 CTGCAC GTGCAG(16/14)
BsiEI 726 CGGCCG CG[GA][CT]^CG
BspEI 788 TCCGGA T^CCGGA
BsrBI 864 CCGCTC CCGCTC(-3/-3)
BsrDI 647 GCAATG GCAATG(2/0)
BsrFI 724 GCCGGC [GA]^CCGG[CT]
BstYI 535 GGATCT [GA]^GATC[CT]
Cfr10I 724 GCCGGC [GA]^CCGG[CT]
DraII 689 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 71 CCGTGG C^C[GA][CT]GG
EaeI 726 CGGCCG [CT]^GGCC[GA]
EagI 726 CGGCCG C^GGCCG
Eam1105I 226 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 360 GAAGAG CTCTTC(1/4)
EclXI 726 CGGCCG C^GGCCG
Eco52I 726 CGGCCG C^GGCCG
EcoO109I 689 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 475 GATATC GAT^ATC
FauI 214 GCGGG CCCGC(4/6)
FbaI 558 TGATCA T^GATCA
KpnI 187 GGTACC GGTAC^C
Ksp632I 360 GAAGAG CTCTTC(1/4)
MaeII 443 ACGT A^CGT
MflI 535 GGATCT [GA]^GATC[CT]
MroI 788 TCCGGA T^CCGGA
MseI 151 TTAA T^TAA
MslI 660 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 724 GCCGGC GCC^GGC
NgoMIV 724 GCCGGC G^CCGGC
PaeR7I 528 CTCGAG C^TCGAG
PpuMI 689 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 594 GACTATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 277 CTGCAG CTGCA^G
SfaNI 382 GATGC GCATC(5/9)
SfcI 277 CTGCAG C^T[GA][CT]AG
SmlI 528 CTCGAG C^T[CT][GA]AG
Tru9I 151 TTAA T^TAA
Tsp509I 89 AATT ^AATT
XhoI 528 CTCGAG C^TCGAG
XhoII 535 GGATCT [GA]^GATC[CT]
XmnI 885 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 188 GTAC GT^AC
402 GTAC
AvaI 504 CTCGGG C^[CT]CG[GA]G
528 CTCGAG
AvaII 68 GGACC G^G[AT]CC
690 GGACC
BmyI 241 GTGCCC G[AGT]GC[ACT]^C
876 GGGCTC
BsmAI 448 GAGAC GTCTC(1/5)
253 GTCTC
BsoBI 504 CTCGGG C^[CT]CG[GA]G
528 CTCGAG
Bsp1286I 241 GTGCCC G[AGT]GC[ACT]^C
876 GGGCTC
BssSI 456 CACGAG CACGAG(-5/-1)
694 CTCGTG
BstF5I 123 CATCC GGATG(2/0)
849 CATCC
Cfr13I 68 GGACC G^G[ACGT]CC
690 GGACC
FokI 123 CATCC GGATG(9/13)
849 CATCC
HaeIII 108 GGCC GG^CC
727 GGCC
HgaI 211 GACGC GACGC(5/10)
232 GCGTC
HphI 553 TCACC GGTGA(8/7)
768 TCACC
MspA1I 261 CCGCTG C[AC]G^C[GT]G
431 CCGCTG
RsaI 188 GTAC GT^AC
402 GTAC
Sau96I 68 GGACC G^G[ACGT]CC
690 GGACC
TaqI 529 TCGA T^CGA
740 TCGA
TfiI 682 GATTC G^A[AT]TC
765 GATTC
Tsp45I 552 GTCAC ^GT[GC]AC
697 GTGAC
TthHB8I 529 TCGA T^CGA
740 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BsmFI BspMI
BsrI BssKI BstNI BstUI Cac8I
CfoI DdeI DpnII EcoRII Fnu4HI
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI TseI TspRI