Restriction map for
pFN21AB4637
(Circular) (Six-base) MAPSORT of: pFN21AB4637.seq Check: 3641 from: 1 to: 5605
pFN21AB4637 5605 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2289 2611 2289
Size: 322 5283
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2192 2926 2192
Size: 734 4871
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 2444 2607 3668 5052 5320 1632
Size: 740 72 163 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 740 268 163 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 2236 4334 2236
Size: 2098 3507
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2261 3348 4920 2261
Size: 1087 1572 2946
Fragments arranged by size:
2946 1572 1087
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2093 2914 3140 3292 5401 1493
Size: 600 821 226 152 2109 1697
Fragments arranged by size:
2109 1697 821 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2509 2533 2930 1870
Size: 73 566 24 397 4545
Fragments arranged by size:
4545 566 397 73 24
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2926 4381 618
Size: 325 217 627 405 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 405 325 217
BanII G_rGCy'C
Cuts at: 1391 2885 2924 1391
Size: 1494 39 4072
Fragments arranged by size:
4072 1494 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 928
Size: 971 189 4445
Fragments arranged by size:
4445 971 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 2563 1548
Size: 1015 4590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2250 3666 4822 1790
Size: 460 1416 1156 2573
Fragments arranged by size:
2573 1416 1156 460
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 2307 3336
Size: 416 53 83 399 24 632 258 1029
Cuts at: 3336 4294 442
Size: 958 1753
Fragments arranged by size:
1753 1029 958 632 416 399 258 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4265 1239
Size: 3026 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 4268 882
Size: 617 948 1821 2219
Fragments arranged by size:
2219 1821 948 617
BsaAI yAC'GTr
Cuts at: 493 1204 2449 3396 493
Size: 711 1245 947 2702
Fragments arranged by size:
2702 1245 947 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2793 4038 4436 4710 4857 5268 2793
Size: 1245 398 274 147 411 3130
Fragments arranged by size:
3130 1245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5605
BseYI C'CCAG_C
Cuts at: 2168 2211 2495 2514 4808 5304 2168
Size: 43 284 19 2294 496 2469
Fragments arranged by size:
2469 2294 496 284 43 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 1163
Size: 573 347 4685
Fragments arranged by size:
4685 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3055 3816 4844 1540
Size: 275 186 733 321 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 3666 3751 4822 3666
Size: 85 1071 4449
Fragments arranged by size:
4449 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2885 2924 3666 3751 4822 1391
Size: 399 460 635 39 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 460 399 85 39
BspEI T'CCGG_A
Cuts at: 2793 5268 2793
Size: 2475 3130
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2611 2714 2960 844
Size: 1445 322 103 246 3489
Fragments arranged by size:
3489 1445 322 246 103
BsrBI CCG'CTC
Cuts at: 1394 1823 2871 3492 1394
Size: 429 1048 621 3507
Fragments arranged by size:
3507 1048 621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2659 4100 4274 65
Size: 1183 1411 1441 174 1396
Fragments arranged by size:
1441 1411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2729 4249 4436 1700
Size: 110 919 1520 187 2869
Fragments arranged by size:
2869 1520 919 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 2461 2699 3665 4677 2461
Size: 238 966 1012 3389
Fragments arranged by size:
3389 1012 966 238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2540 2935 3302 3687 3704 5156 5242 5254
Size: 568 395 367 385 17 1452 86 12
Cuts at: 5254 5600 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 568 395 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 513
Size: 552 939 72 4042
Fragments arranged by size:
4042 939 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2218 487
Size: 683 56 992 3874
Fragments arranged by size:
3874 992 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 3121 3915 3935 2184
Size: 937 794 20 3854
Fragments arranged by size:
3854 937 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 3052 3941
Size: 54 1425 50 275 18 901 321 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 901 889 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 3052 1537
Size: 275 919 321 4090
Fragments arranged by size:
4090 919 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 3535 1353
Size: 1007 1175 3423
Fragments arranged by size:
3423 1175 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2846 4192 4566 4712 387
Size: 1064 138 1257 1346 374 146 1280
Fragments arranged by size:
1346 1280 1257 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2444 2607 3668 4265 5052 5320
Size: 393 740 72 163 1061 597 787 268
Cuts at: 5320 1239
Size: 1524
Fragments arranged by size:
1524 1061 787 740 597 393 268 163
72
EcoO109I rG'GnC_Cy
Cuts at: 2695 2695
Size: 5605
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
EcoRV GAT'ATC
Cuts at: 2482 2482
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2222 2254 2492
Size: 32 135 32 428 32 190 32 238
Cuts at: 2492 2516 2524 2548 4246 4278 4320 4352 1373
Size: 24 8 24 1698 32 42 32 2626
Fragments arranged by size:
2626 1698 428 238 190 135 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2196 2930 2196
Size: 734 4871
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 4719 4903 1305
Size: 1584 1830 184 2007
Fragments arranged by size:
2007 1830 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 3563 3922 4081 518
Size: 648 1503 894 359 159 2042
Fragments arranged by size:
2042 1503 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2268 2438 3021 3698 4846 5091 1606
Size: 662 170 583 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 583 245 170
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 5605
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2885 2898 2885
Size: 13 5592
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5605
PshAI GACnn'nnGTC
Cuts at: 1613 2603 1613
Size: 990 4615
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspXI vC'TCGA_Gb
Cuts at: 2533 2533
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2286 2957 830
Size: 1456 671 3478
Fragments arranged by size:
3478 1456 671
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2282 2953 4088 4769 4960 826
Size: 219 1237 671 1135 681 191 1471
Fragments arranged by size:
1471 1237 1135 681 671 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2533 3709 4610 4872 820
Size: 197 926 590 1176 901 262 1553
Fragments arranged by size:
1553 1176 926 901 590 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 3044 513
Size: 552 939 1040 3074
Fragments arranged by size:
3074 1040 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2607 2639 5565 5597 2607
Size: 32 2926 32 2615
Fragments arranged by size:
2926 2615 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 2533 1943
Size: 590 5015
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 2894 3734 2894
Size: 840 4765
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI