Restriction map for
pFN21AB4637
(Linear) (Six-base) MAPSORT of: KIBB4637.seq Check: 3922 from: 1 to: 900
KIBB4637.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 284 606 900
Size: 284 322 294
Fragments arranged by size:
322 294 284
Acc65I G'GTAC_C
Cuts at: 0 187 900
Size: 187 713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 439 602 900
Size: 367 72 163 298
Fragments arranged by size:
367 298 163 72
AhdI GACnn_n'nnGTC
Cuts at: 0 231 900
Size: 231 669
AlwNI CAG_nnn'CTG
Cuts at: 0 256 900
Size: 256 644
ApoI r'AATT_y
Cuts at: 0 88 900
Size: 88 812
AvaI C'yCGr_G
Cuts at: 0 504 528 900
Size: 504 24 372
Fragments arranged by size:
504 372 24
BanI G'GyrC_C
Cuts at: 0 187 900
Size: 187 713
BanII G_rGCy'C
Cuts at: 0 880 900
Size: 880 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 900
Size: 83 817
BclI T'GATC_A
Cuts at: 0 558 900
Size: 558 342
Bme1580I G_kGCm'C
Cuts at: 0 245 900
Size: 245 655
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 302 900
Size: 44 258 598
Fragments arranged by size:
598 258 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 900
Size: 60 840
BsaI GGTCTCn'nnnn_
Cuts at: 0 442 900
Size: 442 458
BsaAI yAC'GTr
Cuts at: 0 444 900
Size: 444 456
BsaWI w'CCGG_w
Cuts at: 0 788 900
Size: 788 112
BseYI C'CCAG_C
Cuts at: 0 163 206 490 509 900
Size: 163 43 284 19 391
Fragments arranged by size:
391 284 163 43 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 900
Size: 78 822
BsiEI CG_ry'CG
Cuts at: 0 729 900
Size: 729 171
Bsp1286I G_dGCh'C
Cuts at: 0 245 880 900
Size: 245 635 20
Fragments arranged by size:
635 245 20
BspEI T'CCGG_A
Cuts at: 0 788 900
Size: 788 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 606 709 900
Size: 284 322 103 191
Fragments arranged by size:
322 284 191 103
BsrBI CCG'CTC
Cuts at: 0 866 900
Size: 866 34
BsrDI GCAATG_nn'
Cuts at: 0 654 900
Size: 654 246
BsrFI r'CCGG_y
Cuts at: 0 724 900
Size: 724 176
BssSI C'ACGA_G
Cuts at: 0 456 694 900
Size: 456 238 206
Fragments arranged by size:
456 238 206
BstYI r'GATC_y
Cuts at: 0 535 900
Size: 535 365
BtgI C'CryG_G
Cuts at: 0 71 900
Size: 71 829
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 213 900
Size: 213 687
BtsI GCAGTG_nn'
Cuts at: 0 179 900
Size: 179 721
EaeI y'GGCC_r
Cuts at: 0 726 900
Size: 726 174
EagI C'GGCC_G
Cuts at: 0 726 900
Size: 726 174
EarI CTCTTCn'nnn_
Cuts at: 0 355 900
Size: 355 545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 841 900
Size: 841 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 439 602 900
Size: 367 72 163 298
Fragments arranged by size:
367 298 163 72
EcoO109I rG'GnC_Cy
Cuts at: 0 690 900
Size: 690 210
EcoRV GAT'ATC
Cuts at: 0 477 900
Size: 477 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 217 249 487 511 519 543 900
Size: 27 190 32 238 24 8 24 357
Fragments arranged by size:
357 238 190 32 27 24 24 8
KpnI G_GTAC'C
Cuts at: 0 191 900
Size: 191 709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 900
Size: 884 16
MslI CAynn'nnrTG
Cuts at: 0 664 900
Size: 664 236
MspA1I CmG'CkG
Cuts at: 0 263 433 900
Size: 263 170 467
Fragments arranged by size:
467 263 170
NaeI GCC'GGC
Cuts at: 0 726 900
Size: 726 174
NgoMIV G'CCGG_C
Cuts at: 0 724 900
Size: 724 176
PfoI T'CCnGG_A
Cuts at: 0 880 893 900
Size: 880 13 7
Fragments arranged by size:
880 13 7
PpuMI rG'GwC_Cy
Cuts at: 0 690 900
Size: 690 210
PshAI GACnn'nnGTC
Cuts at: 0 598 900
Size: 598 302
PspXI vC'TCGA_Gb
Cuts at: 0 528 900
Size: 528 372
PstI C_TGCA'G
Cuts at: 0 281 900
Size: 281 619
SfcI C'TryA_G
Cuts at: 0 277 900
Size: 277 623
SmlI C'TyrA_G
Cuts at: 0 528 900
Size: 528 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 602 634 900
Size: 602 32 266
Fragments arranged by size:
602 266 32
XhoI C'TCGA_G
Cuts at: 0 528 900
Size: 528 372
XmnI GAAnn'nnTTC
Cuts at: 0 889 900
Size: 889 11
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlwNI ApoI AvaI BanI
BanII BbsI BclI Bme1580I BmrI BplI BsaI BsaAI
BsaWI BseYI BsgI BsiEI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRV Hin4I KpnI
MmeI MslI MspA1I NaeI NgoMIV PfoI PpuMI PshAI
PspXI PstI SfcI SmlI TstI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XmaI ZraI