Restriction map for
pFN21AB4629
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoT14I FbaI FseI
HgaI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RsrII SacII SalI SapI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 1096 AACGTT AA^CGTT
AcyI 624 GGCGCC G[GA]^CG[CT]C
AflIII 1316 ACATGT A^C[GA][CT]GT
AocI 25 CCTGAGG CC^T[ACGT]AGG
AviII 950 TGCGCA TGC^GCA
BanI 624 GGCGCC G^G[CT][GA]CC
BbeI 624 GGCGCC GGCGC^C
BglI 874 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1304 AGATCT A^GATCT
BsaHI 624 GGCGCC G[GA]^CG[CT]C
BsgI 1285 CTGCAC GTGCAG(16/14)
BsiWI 298 CGTACG C^GTACG
BspHI 1091 TCATGA T^CATGA
BspLU11I 1316 ACATGT A^CATGT
BsrDI 1053 CATTGC GCAATG(2/0)
BssSI 859 CACGAG CACGAG(-5/-1)
BstYI 1304 AGATCT [GA]^GATC[CT]
Bsu36I 25 CCTGAGG CC^T[ACGT]AGG
DraII 190 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1102 CCACGG C^C[GA][CT]GG
Eco81I 25 CCTGAGG CC^T[ACGT]AGG
EcoO109I 190 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1144 GAATTC G^AATTC
EcoRV 976 GATATC GAT^ATC
EcoT22I 266 ATGCAT ATGCA^T
FspI 950 TGCGCA TGC^GCA
HaeII 624 GGCGCC [GA]GCGC^[CT]
Hin1I 624 GGCGCC G[GA]^CG[CT]C
HincII 892 GTCAAC GT[CT]^[GA]AC
HindII 892 GTCAAC GT[CT]^[GA]AC
HinfI 348 GATTC G^A[ACGT]TC
KasI 624 GGCGCC G^GCGCC
MflI 1304 AGATCT [GA]^GATC[CT]
NarI 624 GGCGCC GG^CGCC
NsiI 266 ATGCAT ATGCA^T
Psp1406I 1096 AACGTT AA^CGTT
PvuII 339 CAGCTG CAG^CTG
RcaI 1091 TCATGA T^CATGA
SbfI 1152 CCTGCAGG CCTGCA^GG
ScaI 377 AGTACT AGT^ACT
SexAI 830 ACCAGGT A^CC[AT]GGT
SfoI 624 GGCGCC GGC^GCC
SmlI 844 CTCAAG C^T[CT][GA]AG
SphI 102 GCATGC GCATG^C
Sse8387I 1152 CCTGCAGG CCTGCA^GG
TfiI 348 GATTC G^A[AT]TC
XhoII 1304 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 997 GAATTT [GA]^AATT[CT]
1144 GAATTC
ApoI 997 GAATTT [GA]^AATT[CT]
1144 GAATTC
Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAAAACTTC
BbsI 1263 GAAGAC GAAGAC(2/6)
733 GTCTTC
BpmI 1297 CTCCAG CTGGAG(16/14)
316 CTGGAG
BpuAI 1263 GAAGAC GAAGAC(2/6)
733 GTCTTC
BsaJI 1019 CCCTGG C^C[ACGT][ACGT]GG
1102 CCACGG
BsmFI 93 GGGAC GGGAC(10/14)
233 GGGAC
BspMI 1151 ACCTGC ACCTGC(4/8)
1214 ACCTGC
BsrFI 604 ACCGGC [GA]^CCGG[CT]
1079 ACCGGC
BstAPI 365 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1287 GCACCCTCTGC
BstEII 834 GGTTACC G^GT[ACGT]ACC
1227 GGTCACC
BstPI 834 GGTTACC G^GT[ACGT]ACC
1227 GGTCACC
Cfr10I 604 ACCGGC [GA]^CCGG[CT]
1079 ACCGGC
EcoO65I 834 GGTTACC G^GT[ACGT]ACC
1227 GGTCACC
MaeII 731 ACGT A^CGT
1097 ACGT
StuI 527 AGGCCT AGG^CCT
1086 AGGCCT
TaqI 647 TCGA T^CGA
1142 TCGA
Tsp509I 998 AATT ^AATT
1145 AATT
TthHB8I 647 TCGA T^CGA
1142 TCGA
XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
ApaLI AspHI AvaII BanII BbvI
BcnI BmyI BsaI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI