(Circular) (Six-base) MAPSORT of: pFN21AB4629.seq Check: 3310 from: 1 to: 6028 pFN21AB4629 6028 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3165 3165 Size: 6028 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3371 1937 Size: 1434 4594 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 3102 4155 4528 1563 Size: 1539 1053 373 3063 Fragments arranged by size: 3063 1539 1053 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2549 2797 2959 4091 5475 5743 1632 Size: 917 248 162 1132 1384 268 1917 Fragments arranged by size: 1917 1384 1132 917 268 248 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 3321 4927 5904 1203 Size: 2118 1606 977 1327 Fragments arranged by size: 2118 1606 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 2262 2762 1166 Size: 1096 500 4432 Fragments arranged by size: 4432 1096 500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 3771 5343 3771 Size: 1572 4456 ApaLI G'TGCA_C Cuts at: 2732 2755 2861 4085 5241 2732 Size: 23 106 1224 1156 3519 Fragments arranged by size: 3519 1224 1156 106 23 ApoI r'AATT_y Cuts at: 1493 3002 3149 3337 3563 3715 5824 1493 Size: 1509 147 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1509 226 188 152 147 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 3353 1870 Size: 73 1410 4545 Fragments arranged by size: 4545 1410 73 BamHI G'GATC_C Cuts at: 3358 3725 3358 Size: 367 5661 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2629 3349 4804 618 Size: 325 217 627 842 720 1455 1842 Fragments arranged by size: 1842 1455 842 720 627 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2091 2150 2784 3347 1391 Size: 692 8 59 634 563 4072 Fragments arranged by size: 4072 692 634 563 59 8 BbeI G_GCGC'C Cuts at: 2633 2633 Size: 6028 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2731 3275 928 Size: 971 832 544 3681 Fragments arranged by size: 3681 971 832 544 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BfrBI ATG'CAT Cuts at: 2273 2273 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2885 4639 136 Size: 107 122 71 631 605 1213 1754 1525 Fragments arranged by size: 1754 1525 1213 631 605 122 107 71 BglII A'GATC_T Cuts at: 3309 6023 3309 Size: 2714 3314 BlpI GC'TnA_GC Cuts at: 3445 3445 Size: 6028 Bme1580I G_kGCm'C Cuts at: 1790 2736 2759 2865 3145 4089 5245 1790 Size: 946 23 106 280 944 1156 2573 Fragments arranged by size: 2573 1156 946 944 280 106 23 BmgBI CAC'GTC Cuts at: 1318 2737 1318 Size: 1419 4609 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 3175 3759 Size: 416 53 83 399 24 1003 755 584 Cuts at: 3759 4717 442 Size: 958 1753 Fragments arranged by size: 1753 1003 958 755 584 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 3287 4688 1239 Size: 1103 945 1401 2579 Fragments arranged by size: 2579 1401 1103 945 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2834 4153 5018 5316 2834 Size: 1319 865 298 3546 Fragments arranged by size: 3546 1319 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2762 3075 3275 4691 882 Size: 617 1263 313 200 1416 2219 Fragments arranged by size: 2219 1416 1263 617 313 200 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2630 3835 4217 4852 Size: 53 83 186 936 1097 1205 382 635 Cuts at: 4852 275 Size: 1451 Fragments arranged by size: 1451 1205 1097 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4461 4859 5133 5280 5691 4461 Size: 398 274 147 411 4798 Fragments arranged by size: 4798 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5803 Fragments arranged by size: 5803 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2063 2140 1186 Size: 874 3 77 5074 Fragments arranged by size: 5074 874 77 3 BseYI C'CCAG_C Cuts at: 2807 5231 5727 2807 Size: 2424 496 3108 Fragments arranged by size: 3108 2424 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3275 1163 Size: 573 1539 3916 Fragments arranged by size: 3916 1539 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 4239 5267 1540 Size: 275 186 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2150 2736 2759 2784 2865 3243 4089 4174 Size: 59 586 23 25 81 378 846 85 Cuts at: 4174 5245 2091 Size: 1071 2874 Fragments arranged by size: 2874 1071 846 586 378 85 81 59 25 23 BsiWI C'GTAC_G Cuts at: 2303 2303 Size: 6028 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2091 2150 2736 2759 2784 2865 Size: 399 293 8 59 586 23 25 81 Cuts at: 2865 3145 3243 3347 4089 4174 5245 1391 Size: 280 98 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 586 399 293 280 104 98 85 81 59 25 23 8 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3096 3917 5647 3096 Size: 821 1730 3477 Fragments arranged by size: 3477 1730 821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3165 3228 3383 844 Size: 2321 63 155 3489 Fragments arranged by size: 3489 2321 155 63 BsrBI CCG'CTC Cuts at: 1394 1823 3915 1394 Size: 429 2092 3507 Fragments arranged by size: 3507 2092 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3057 4523 4697 65 Size: 1183 1809 1466 174 1396 Fragments arranged by size: 1809 1466 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2609 3084 4672 4859 1700 Size: 110 799 475 1588 187 2869 Fragments arranged by size: 2869 1588 799 475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 2864 4088 5100 2864 Size: 1224 1012 3792 Fragments arranged by size: 3792 1224 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2376 3298 794 Size: 1582 922 3524 Fragments arranged by size: 3524 1582 922 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 2839 3232 1184 Size: 1655 393 3980 Fragments arranged by size: 3980 1655 393 BstYI r'GATC_y Cuts at: 1972 3309 3358 3725 4110 4127 5579 5665 5677 Size: 1337 49 367 385 17 1452 86 12 Cuts at: 5677 6023 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1337 385 367 346 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2031 2031 Size: 6028 BtgI C'CryG_G Cuts at: 513 1065 2004 3107 513 Size: 552 939 1103 3434 Fragments arranged by size: 3434 1103 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 2162 2935 3544 4338 4358 2162 Size: 773 609 794 20 3832 Fragments arranged by size: 3832 794 773 609 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2911 2946 529 Size: 35 2347 35 3611 Fragments arranged by size: 3611 2347 35 35 DraI TTT'AAA Cuts at: 3332 3332 Size: 6028 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 3063 3086 Size: 54 1425 50 275 18 690 543 23 Cuts at: 3086 3475 4364 5993 8 Size: 389 889 1629 43 Fragments arranged by size: 1629 1425 889 690 543 389 275 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 3475 1537 Size: 275 1663 4090 Fragments arranged by size: 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2456 2828 3194 3958 1353 Size: 684 419 372 366 764 3423 Fragments arranged by size: 3423 764 684 419 372 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4615 4989 5135 387 Size: 1064 138 3026 374 146 1280 Fragments arranged by size: 3026 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2089 2148 2782 2089 Size: 59 634 5335 Fragments arranged by size: 5335 634 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2549 2797 2959 3287 4091 4688 Size: 393 710 207 248 162 328 804 597 Cuts at: 4688 5475 5743 1239 Size: 787 268 1524 Fragments arranged by size: 1524 804 787 710 597 393 328 268 248 207 162 EcoO109I rG'GnC_Cy Cuts at: 2196 2196 Size: 6028 EcoRI G'AATT_C Cuts at: 3149 3337 3715 3149 Size: 188 378 5462 Fragments arranged by size: 5462 378 188 EcoRV GAT'ATC Cuts at: 2983 2983 Size: 6028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 6028 FspI TGC'GCA Cuts at: 2957 4534 2957 Size: 1577 4451 HaeII r_GCGC'y Cuts at: 1678 2633 5175 1678 Size: 955 2542 2531 Fragments arranged by size: 2542 2531 955 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2231 2263 4669 Size: 32 135 32 611 32 16 32 2406 Cuts at: 4669 4701 4743 4775 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2406 611 135 42 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2899 3372 3618 669 Size: 988 281 961 473 246 3079 Fragments arranged by size: 3079 988 961 473 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6028 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KasI G'GCGC_C Cuts at: 2629 2629 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 3627 3627 Size: 6028 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2423 5142 5326 1305 Size: 1118 2719 184 2007 Fragments arranged by size: 2719 2007 1118 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4206 Fragments arranged by size: 4206 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2262 2274 2762 3174 3986 4345 4504 Size: 648 1096 12 488 412 812 359 159 Cuts at: 4504 518 Size: 2042 Fragments arranged by size: 2042 1096 812 648 488 412 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2346 2748 3259 3444 4121 5269 5514 1606 Size: 740 402 511 185 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 511 402 245 185 NarI GG'CG_CC Cuts at: 2630 2630 Size: 6028 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NsiI A_TGCA'T Cuts at: 2275 2275 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 2111 2277 2419 3325 3386 4931 1132 Size: 97 882 166 142 906 61 1545 2229 Fragments arranged by size: 2229 1545 906 882 166 142 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 3321 4927 3321 Size: 1606 4422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6028 PfoI T'CCnGG_A Cuts at: 2037 2141 3123 3303 2037 Size: 104 982 180 4762 Fragments arranged by size: 4762 982 180 104 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4129 5610 5642 4097 Size: 32 1481 32 4483 Fragments arranged by size: 4483 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 3598 3598 Size: 6028 PstI C_TGCA'G Cuts at: 830 2181 3162 3207 3380 830 Size: 1351 981 45 173 3478 Fragments arranged by size: 3478 1351 981 173 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 PvuII CAG'CTG Cuts at: 2346 2346 Size: 6028 SacI G_AGCT'C Cuts at: 2091 2150 2784 2091 Size: 59 634 5335 Fragments arranged by size: 5335 634 59 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SbfI CC_TGCA'GG Cuts at: 3162 3380 3162 Size: 218 5810 ScaI AGT'ACT Cuts at: 2384 4276 5988 2384 Size: 1892 1712 2424 Fragments arranged by size: 2424 1892 1712 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 6028 SfcI C'TryA_G Cuts at: 826 1045 2177 3158 3203 3376 4511 5192 5383 Size: 219 1132 981 45 173 1135 681 191 Cuts at: 5383 826 Size: 1471 Fragments arranged by size: 1471 1135 1132 981 681 219 191 173 45 SfoI GGC'GCC Cuts at: 2631 2631 Size: 6028 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 3355 1872 Size: 1483 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2849 4132 5033 5295 820 Size: 197 926 906 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 906 901 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 2111 3386 1132 Size: 979 1275 3774 Fragments arranged by size: 3774 1275 979 SspI AAT'ATT Cuts at: 5 52 3952 5 Size: 47 3900 2081 Fragments arranged by size: 3900 2081 47 StuI AGG'CCT Cuts at: 1517 2534 3093 1517 Size: 1017 559 4452 Fragments arranged by size: 4452 1017 559 StyI C'CwwG_G Cuts at: 513 1065 2004 3467 513 Size: 552 939 1463 3074 Fragments arranged by size: 3074 1463 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3287 4055 4072 4225 4410 4855 325 Size: 1255 1707 768 17 153 185 445 1498 Fragments arranged by size: 1707 1498 1255 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 2505 4274 Size: 274 80 33 51 1443 405 123 1769 Cuts at: 4274 5986 96 Size: 1712 138 Fragments arranged by size: 1769 1712 1443 405 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5988 6020 5988 Size: 32 5996 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6028 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 3353 1870 Size: 1483 4545 XmnI GAAnn'nnTTC Cuts at: 2534 2570 4157 2534 Size: 36 1587 4405 Fragments arranged by size: 4405 1587 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI