(Circular) (Six-base) MAPSORT of: pFN21AB4619.seq Check: 5757 from: 1 to: 5857 pFN21AB4619 5857 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 3200 1937 Size: 927 336 4594 Fragments arranged by size: 4594 927 336 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 2297 4756 5733 1203 Size: 1094 2459 977 1327 Fragments arranged by size: 2459 1327 1094 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2339 3600 5172 2339 Size: 1261 1572 3024 Fragments arranged by size: 3024 1572 1261 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 2415 2738 3166 3392 3544 5653 1493 Size: 922 323 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 428 323 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 3182 1870 Size: 73 1239 4545 Fragments arranged by size: 4545 1239 73 AvrII C'CTAG_G Cuts at: 2909 2909 Size: 5857 BamHI G'GATC_C Cuts at: 2314 2833 3187 3554 2314 Size: 519 354 367 4617 Fragments arranged by size: 4617 519 367 354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3178 4633 618 Size: 325 217 627 1391 1455 1842 Fragments arranged by size: 1842 1455 1391 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 3176 1391 Size: 751 1034 4072 Fragments arranged by size: 4072 1034 751 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2198 3749 4959 2198 Size: 1551 1210 3096 Fragments arranged by size: 3096 1551 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 3918 5074 1790 Size: 2128 1156 2573 Fragments arranged by size: 2573 2128 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2834 3588 4546 Size: 416 53 83 399 24 1417 754 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1417 958 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2649 2871 2913 3123 4517 1239 Size: 1410 222 42 210 1394 2579 Fragments arranged by size: 2579 1410 1394 222 210 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 2250 5851 2250 Size: 3601 2256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 3664 4046 4681 Size: 53 83 186 936 747 1384 382 635 Cuts at: 4681 275 Size: 1451 Fragments arranged by size: 1451 1384 936 747 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2482 2512 2869 Size: 30 165 30 678 30 371 30 357 Cuts at: 2869 2899 3021 3051 1178 Size: 30 122 30 3984 Fragments arranged by size: 3984 678 371 357 165 122 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2211 1186 Size: 940 85 4832 Fragments arranged by size: 4832 940 85 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2568 1163 Size: 573 832 4452 Fragments arranged by size: 4452 832 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 3307 4068 5096 1540 Size: 275 186 19 1287 761 1028 2301 Fragments arranged by size: 2301 1287 1028 761 275 186 19 BsiHKAI G_wGCw'C Cuts at: 2142 2624 3918 4003 5074 2142 Size: 482 1294 85 1071 2925 Fragments arranged by size: 2925 1294 1071 482 85 BsmI GAATG_Cn' Cuts at: 2774 3368 3461 2774 Size: 594 93 5170 Fragments arranged by size: 5170 594 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2624 3176 3918 4003 5074 1391 Size: 399 352 482 552 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 482 399 352 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3212 844 Size: 2368 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2457 3744 1394 Size: 429 634 1287 3507 Fragments arranged by size: 3507 1287 634 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2997 4352 4526 65 Size: 1183 1749 1355 174 1396 Fragments arranged by size: 1749 1396 1355 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2489 96 Size: 2393 3464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2339 794 Size: 1545 4312 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2314 2596 2833 3187 3554 3939 3956 5408 Size: 342 282 237 354 367 385 17 1452 Cuts at: 5408 5494 5506 5852 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 354 346 342 282 237 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2902 2961 2902 Size: 59 5798 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 513 Size: 552 939 69 4297 Fragments arranged by size: 4297 939 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 2322 3161 2322 Size: 839 5018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2807 4864 809 Size: 507 1491 2057 1802 Fragments arranged by size: 2057 1802 1491 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2070 2326 Size: 54 1425 50 275 18 187 53 256 Cuts at: 2326 3304 4193 5822 8 Size: 978 889 1629 43 Fragments arranged by size: 1629 1425 978 889 275 256 187 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2017 3304 1537 Size: 275 205 1287 4090 Fragments arranged by size: 4090 1287 275 205 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2649 2871 2913 3123 3920 4517 5304 Size: 393 1017 222 42 210 797 597 787 Cuts at: 5304 5572 1239 Size: 268 1524 Fragments arranged by size: 1524 1017 797 787 597 393 268 222 210 42 EcoNI CCTnn'n_nnAGG Cuts at: 2964 2964 Size: 5857 EcoO109I rG'GnC_Cy Cuts at: 2906 2906 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 EcoRV GAT'ATC Cuts at: 2721 2951 3062 2721 Size: 230 111 5516 Fragments arranged by size: 5516 230 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2990 2990 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2050 5004 1678 Size: 372 2954 2531 Fragments arranged by size: 2954 2531 372 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373 Size: 32 135 32 2926 32 42 32 2626 Fragments arranged by size: 2926 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3201 3447 669 Size: 988 281 927 336 246 3079 Fragments arranged by size: 3079 988 927 336 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 2805 3456 2805 Size: 651 5206 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3007 4971 5155 1305 Size: 1702 1964 184 2007 Fragments arranged by size: 2007 1964 1702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2328 10 Size: 54 1425 343 496 3539 Fragments arranged by size: 3539 1425 496 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3002 3815 4174 4333 518 Size: 648 1836 813 359 159 2042 Fragments arranged by size: 2042 1836 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3273 3950 5098 5343 1606 Size: 1667 677 1148 245 2120 Fragments arranged by size: 2120 1667 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2073 513 Size: 552 939 69 4297 Fragments arranged by size: 4297 939 552 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2301 3215 4760 1132 Size: 97 1072 914 1545 2229 Fragments arranged by size: 2229 1545 1072 914 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 2297 4756 2297 Size: 2459 3398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PfoI T'CCnGG_A Cuts at: 2944 2944 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2906 2906 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 2190 3427 2190 Size: 1237 4620 PstI C_TGCA'G Cuts at: 830 3209 830 Size: 2379 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 SacI G_AGCT'C Cuts at: 2142 2142 Size: 5857 SalI G'TCGA_C Cuts at: 1936 2863 3199 1936 Size: 927 336 4594 Fragments arranged by size: 4594 927 336 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 2827 4105 5817 2827 Size: 1278 1712 2867 Fragments arranged by size: 2867 1712 1278 SfcI C'TryA_G Cuts at: 826 1045 3205 4340 5021 5212 826 Size: 219 2160 1135 681 191 1471 Fragments arranged by size: 2160 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 2914 1517 Size: 1397 4460 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2909 3296 513 Size: 552 939 69 836 387 3074 Fragments arranged by size: 3074 939 836 552 387 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 3884 3901 4054 4239 4684 325 Size: 1255 666 1638 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1255 666 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 2489 2825 Size: 274 80 33 51 1443 111 401 336 Cuts at: 2825 4103 5815 96 Size: 1278 1712 138 Fragments arranged by size: 1712 1443 1278 401 336 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 2415 3986 2415 Size: 1571 4286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI