(Circular) (Six-base) MAPSORT of: pFN21AB4609.seq Check: 3258 from: 1 to: 5539 pFN21AB4609 5539 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2882 1937 Size: 945 4594 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2566 2791 3602 4986 5254 1632 Size: 677 257 225 811 1384 268 1917 Fragments arranged by size: 1917 1384 811 677 268 257 225 AfeI AGC'GCT Cuts at: 2097 2429 2097 Size: 332 5207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 2054 4268 2054 Size: 2214 3325 AleI CACnn'nnGTG Cuts at: 1166 2063 1166 Size: 897 4642 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2705 1184 Size: 1521 4018 AlwNI CAG_nnn'CTG Cuts at: 2169 2491 3282 4854 2169 Size: 322 791 1572 2854 Fragments arranged by size: 2854 1572 791 322 ApaLI G'TGCA_C Cuts at: 2594 2624 3596 4752 2594 Size: 30 972 1156 3381 Fragments arranged by size: 3381 1156 972 30 ApoI r'AATT_y Cuts at: 1493 2848 3074 3226 5335 1493 Size: 1355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 226 152 AseI AT'TA_AT Cuts at: 160 1032 2274 4093 160 Size: 872 1242 1819 1606 Fragments arranged by size: 1819 1606 1242 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2864 1870 Size: 73 921 4545 Fragments arranged by size: 4545 921 73 BamHI G'GATC_C Cuts at: 2203 2869 3236 2203 Size: 666 367 4506 Fragments arranged by size: 4506 666 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 2860 4315 618 Size: 325 217 627 624 449 1455 1842 Fragments arranged by size: 1842 1455 627 624 449 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2858 1391 Size: 751 716 4072 Fragments arranged by size: 4072 751 716 BbeI G_GCGC'C Cuts at: 2415 2415 Size: 5539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BbvCI CC'TCA_GC Cuts at: 2221 2221 Size: 5539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2770 2804 3730 3764 1903 Size: 34 50 34 749 34 926 34 3678 Fragments arranged by size: 3678 926 749 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2227 3431 4641 2227 Size: 1204 1210 3125 Fragments arranged by size: 3125 1210 1204 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BfrBI ATG'CAT Cuts at: 2635 2635 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 2373 4150 Size: 107 122 71 631 605 647 54 1777 Cuts at: 4150 136 Size: 1525 Fragments arranged by size: 1777 1525 647 631 605 122 107 71 54 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2956 2956 Size: 5539 Bme1580I G_kGCm'C Cuts at: 1790 2598 2628 3600 4756 1790 Size: 808 30 972 1156 2573 Fragments arranged by size: 2573 1156 972 808 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5539 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4199 1239 Size: 2960 2579 Bpu10I CC'TnA_GC Cuts at: 2015 2221 2015 Size: 206 5333 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3664 4529 4827 3664 Size: 865 298 4376 Fragments arranged by size: 4376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 882 Size: 617 2703 2219 Fragments arranged by size: 2703 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2623 3330 493 Size: 711 1419 707 2702 Fragments arranged by size: 2702 1419 711 707 BsaBI GATnn'nnATC Cuts at: 5533 5533 Size: 5539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2412 3346 3728 4363 Size: 53 83 186 936 879 934 382 635 Cuts at: 4363 275 Size: 1451 Fragments arranged by size: 1451 936 934 879 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4370 4644 4791 5202 3972 Size: 398 274 147 411 4309 Fragments arranged by size: 4309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5539 BseYI C'CCAG_C Cuts at: 2672 4742 5238 2672 Size: 2070 496 2973 Fragments arranged by size: 2973 2070 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 1163 Size: 573 714 4252 Fragments arranged by size: 4252 714 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2239 2544 2989 3750 4778 1540 Size: 275 186 238 305 445 761 1028 2301 Fragments arranged by size: 2301 1028 761 445 305 275 238 186 BsiHKAI G_wGCw'C Cuts at: 2142 2598 2628 3600 3685 4756 2142 Size: 456 30 972 85 1071 2925 Fragments arranged by size: 2925 1071 972 456 85 30 BsiWI C'GTAC_G Cuts at: 2619 2619 Size: 5539 BsmI GAATG_Cn' Cuts at: 3050 3143 3050 Size: 93 5446 BsmBI CGTCTCn'nnnn_ Cuts at: 2609 2609 Size: 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2598 2628 2858 3600 3685 4756 Size: 399 352 456 30 230 742 85 1071 Cuts at: 4756 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 456 399 352 230 85 30 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2894 844 Size: 2050 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3426 1394 Size: 429 1603 3507 Fragments arranged by size: 3507 1603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4034 4208 65 Size: 1183 2786 174 1396 Fragments arranged by size: 2786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2543 4183 4370 1700 Size: 110 733 1640 187 2869 Fragments arranged by size: 2869 1640 733 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5539 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 2632 794 Size: 1838 3701 BstBI TT'CG_AA Cuts at: 2499 4343 2499 Size: 1844 3695 BstEII G'GTnAC_C Cuts at: 1184 2056 1184 Size: 872 4667 BstYI r'GATC_y Cuts at: 1972 2124 2203 2869 3236 3621 3638 5090 5176 Size: 152 79 666 367 385 17 1452 86 Cuts at: 5176 5188 5534 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 666 385 367 346 152 86 79 17 12 Bsu36I CC'TnA_GG Cuts at: 2370 2370 Size: 5539 BtgI C'CryG_G Cuts at: 513 1065 2061 513 Size: 552 996 3991 Fragments arranged by size: 3991 996 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 2015 3055 3849 3869 2015 Size: 1040 794 20 3685 Fragments arranged by size: 3685 1040 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2236 2545 Size: 54 1425 50 275 18 330 76 309 Cuts at: 2545 2812 2986 3875 5504 8 Size: 267 174 889 1629 43 Fragments arranged by size: 1629 1425 889 330 309 275 267 174 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2236 2986 1537 Size: 275 424 750 4090 Fragments arranged by size: 4090 750 424 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2233 2501 2753 3469 1353 Size: 880 268 252 716 3423 Fragments arranged by size: 3423 880 716 268 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4126 4500 4646 387 Size: 1064 138 2537 374 146 1280 Fragments arranged by size: 2537 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 5539 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 2566 2791 3602 4199 4986 5254 Size: 393 677 257 225 811 597 787 268 Cuts at: 5254 1239 Size: 1524 Fragments arranged by size: 1524 811 787 677 597 393 268 257 225 EcoNI CCTnn'n_nnAGG Cuts at: 2822 2822 Size: 5539 EcoO109I rG'GnC_Cy Cuts at: 2081 2194 2305 2348 2081 Size: 113 111 43 5272 Fragments arranged by size: 5272 113 111 43 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 5539 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2099 2415 2431 2758 4686 1678 Size: 421 316 16 327 1928 2531 Fragments arranged by size: 2531 1928 421 327 316 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 4180 4212 4254 Size: 32 135 32 468 32 2108 32 42 Cuts at: 4254 4286 1373 Size: 32 2626 Fragments arranged by size: 2626 2108 468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 3129 669 Size: 988 281 945 246 3079 Fragments arranged by size: 3079 988 945 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5539 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KasI G'GCGC_C Cuts at: 2411 2411 Size: 5539 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 10 Size: 54 1425 343 330 3387 Fragments arranged by size: 3387 1425 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3497 3856 4015 518 Size: 648 897 1434 359 159 2042 Fragments arranged by size: 2042 1434 897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2166 2472 2601 2811 2955 3632 4780 5025 Size: 560 306 129 210 144 677 1148 245 Cuts at: 5025 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 560 306 245 210 144 129 NarI GG'CG_CC Cuts at: 2412 2412 Size: 5539 NcoI C'CATG_G Cuts at: 513 1065 2061 513 Size: 552 996 3991 Fragments arranged by size: 3991 996 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NsiI A_TGCA'T Cuts at: 2637 2637 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2897 4442 1132 Size: 97 1668 1545 2229 Fragments arranged by size: 2229 1668 1545 97 PasI CC'CwG_GG Cuts at: 1348 2077 1348 Size: 729 4810 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4637 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2194 2305 2348 2081 Size: 113 111 43 5272 Fragments arranged by size: 5272 113 111 43 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 5507 PstI C_TGCA'G Cuts at: 830 2891 830 Size: 2061 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2601 2601 Size: 5539 SacI G_AGCT'C Cuts at: 2142 2142 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SanDI GG'GwC_CC Cuts at: 2081 2305 2348 2081 Size: 224 43 5272 Fragments arranged by size: 5272 224 43 SapI GCTCTTCn'nnn_ Cuts at: 2501 2753 2501 Size: 252 5287 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SfcI C'TryA_G Cuts at: 826 1045 2776 2887 4022 4703 4894 826 Size: 219 1731 111 1135 681 191 1471 Fragments arranged by size: 1731 1471 1135 681 219 191 111 SfiI GGCCn_nnn'nGGCC Cuts at: 2319 2319 Size: 5539 SfoI GGC'GCC Cuts at: 2413 2413 Size: 5539 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2866 1872 Size: 994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3643 4544 4806 820 Size: 197 926 1700 901 262 1553 Fragments arranged by size: 1700 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5539 StyI C'CwwG_G Cuts at: 513 1065 2061 2353 2978 513 Size: 552 996 292 625 3074 Fragments arranged by size: 3074 996 625 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 2728 3566 3583 3736 3921 4366 Size: 1255 934 214 838 17 153 185 445 Cuts at: 4366 325 Size: 1498 Fragments arranged by size: 1498 1255 934 838 445 214 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3785 5497 96 Size: 274 80 33 51 1443 1808 1712 138 Fragments arranged by size: 1808 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 2732 1316 Size: 1416 4123 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2360 2360 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2864 1870 Size: 994 4545 XmnI GAAnn'nnTTC Cuts at: 2429 3668 2429 Size: 1239 4300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BplI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SexAI SrfI SwaI