(Circular) (Six-base) MAPSORT of: pFN21AB4604.seq Check: 5589 from: 1 to: 5614 pFN21AB4604 5614 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2739 2935 2739 Size: 196 5418 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3677 5061 5329 1632 Size: 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 2679 4513 5490 1203 Size: 1476 1834 977 1327 Fragments arranged by size: 1834 1476 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AlwNI CAG_nnn'CTG Cuts at: 2584 3357 4929 2584 Size: 773 1572 3269 Fragments arranged by size: 3269 1572 773 ApaLI G'TGCA_C Cuts at: 2392 3671 4827 2392 Size: 1279 1156 3179 Fragments arranged by size: 3179 1279 1156 ApoI r'AATT_y Cuts at: 1493 2165 2489 2923 3149 3301 5410 1493 Size: 672 324 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 672 434 324 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2655 2939 1870 Size: 73 712 284 4545 Fragments arranged by size: 4545 712 284 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2109 2142 2109 Size: 33 5581 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2405 2739 2935 4390 618 Size: 325 217 627 618 334 196 1455 1842 Fragments arranged by size: 1842 1455 627 618 334 325 217 196 BanII G_rGCy'C Cuts at: 1391 2933 1391 Size: 1542 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BbvCI CC'TCA_GC Cuts at: 2061 2061 Size: 5614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2045 4225 Size: 107 122 71 631 605 337 36 2180 Cuts at: 4225 136 Size: 1525 Fragments arranged by size: 2180 1525 631 605 337 122 107 71 36 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2396 2408 3675 4831 1790 Size: 606 12 1267 1156 2573 Fragments arranged by size: 2573 1267 1156 606 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4303 442 Size: 416 53 83 399 24 1928 958 1753 Fragments arranged by size: 1928 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2642 4274 1239 Size: 1181 222 1632 2579 Fragments arranged by size: 2579 1632 1181 222 Bpu10I CC'TnA_GC Cuts at: 2061 2061 Size: 5614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 3739 4604 4902 2501 Size: 1238 865 298 3213 Fragments arranged by size: 3213 1238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2625 2810 4277 882 Size: 617 1126 185 1467 2219 Fragments arranged by size: 2219 1467 1126 617 185 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4047 4445 4719 4866 5277 4047 Size: 398 274 147 411 4384 Fragments arranged by size: 4384 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 1186 Size: 1024 4590 BseYI C'CCAG_C Cuts at: 2308 4817 5313 2308 Size: 2509 496 2609 Fragments arranged by size: 2609 2509 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2199 2359 1163 Size: 573 463 160 4418 Fragments arranged by size: 4418 573 463 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2396 2776 3675 3760 4831 2396 Size: 380 899 85 1071 3179 Fragments arranged by size: 3179 1071 899 380 85 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2408 2776 2933 3675 3760 4831 Size: 399 606 12 368 157 742 85 1071 Cuts at: 4831 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 606 399 368 157 85 12 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2969 844 Size: 2125 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2350 2794 4109 4283 65 Size: 1183 1102 444 1315 174 1396 Fragments arranged by size: 1396 1315 1183 1102 444 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2864 4258 4445 1700 Size: 110 1054 1394 187 2869 Fragments arranged by size: 2869 1394 1054 187 110 BsrGI T'GTAC_A Cuts at: 96 2682 2695 96 Size: 2586 13 3015 Fragments arranged by size: 3015 2586 13 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 3674 4686 3674 Size: 1012 4602 BstAPI GCAn_nnn'nTGC Cuts at: 794 2250 2584 794 Size: 1456 334 3824 Fragments arranged by size: 3824 1456 334 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 2530 1184 Size: 1346 4268 BstYI r'GATC_y Cuts at: 1972 2016 2598 2902 2944 3311 3696 3713 5165 Size: 44 582 304 42 367 385 17 1452 Cuts at: 5165 5251 5263 5609 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 582 385 367 346 304 86 44 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 2787 513 Size: 552 1575 147 3340 Fragments arranged by size: 3340 1575 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2830 487 Size: 683 56 1604 3271 Fragments arranged by size: 3271 1604 683 56 BtsI GCAGTG_nn' Cuts at: 2230 2765 3130 3924 3944 2230 Size: 535 365 794 20 3900 Fragments arranged by size: 3900 794 535 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DraIII CAC_nnn'GTG Cuts at: 2391 2641 2771 2391 Size: 250 130 5234 Fragments arranged by size: 5234 250 130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2710 3061 Size: 54 1425 50 275 18 207 673 351 Cuts at: 3061 3950 5579 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 673 351 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 2371 3544 1353 Size: 834 184 1173 3423 Fragments arranged by size: 3423 1173 834 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 4201 4575 4721 387 Size: 1064 138 633 1979 374 146 1280 Fragments arranged by size: 1979 1280 1064 633 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2642 3677 4274 5061 5329 1239 Size: 393 788 222 1035 597 787 268 1524 Fragments arranged by size: 1524 1035 788 787 597 393 268 222 EcoO109I rG'GnC_Cy Cuts at: 2010 2046 2293 2010 Size: 36 247 5331 Fragments arranged by size: 5331 247 36 EcoRI G'AATT_C Cuts at: 2489 2923 3301 2489 Size: 434 378 4802 Fragments arranged by size: 4802 434 378 EcoRV GAT'ATC Cuts at: 2893 2893 Size: 5614 FspI TGC'GCA Cuts at: 2501 4120 2501 Size: 1619 3995 HaeII r_GCGC'y Cuts at: 1678 2620 4761 1678 Size: 942 2141 2531 Fragments arranged by size: 2531 2141 942 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373 Size: 32 135 32 2683 32 42 32 2626 Fragments arranged by size: 2683 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2743 2939 2743 Size: 196 5418 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3792 Fragments arranged by size: 3792 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2282 2607 2786 3572 3931 4090 Size: 648 1110 6 325 179 786 359 159 Cuts at: 4090 518 Size: 2042 Fragments arranged by size: 2042 1110 786 648 359 325 179 159 6 MspA1I CmG'CkG Cuts at: 1606 2304 2716 3030 3707 4855 5100 1606 Size: 698 412 314 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 412 314 245 NaeI GCC'GGC Cuts at: 2866 2866 Size: 5614 NcoI C'CATG_G Cuts at: 513 1065 2787 513 Size: 552 1722 3340 Fragments arranged by size: 3340 1722 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NgoMIV G'CCGG_C Cuts at: 2864 2864 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2506 2683 2972 4517 1132 Size: 97 1277 177 289 1545 2229 Fragments arranged by size: 2229 1545 1277 289 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 2679 4513 2679 Size: 1834 3780 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 4081 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2293 Size: 5614 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PstI C_TGCA'G Cuts at: 830 2966 830 Size: 2136 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 PvuII CAG'CTG Cuts at: 2304 2716 2304 Size: 412 5202 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2962 4097 4778 4969 826 Size: 219 1917 1135 681 191 1471 Fragments arranged by size: 1917 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2045 2045 Size: 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2657 2941 1872 Size: 785 284 4545 Fragments arranged by size: 4545 785 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3718 4619 4881 820 Size: 197 926 573 1202 901 262 1553 Fragments arranged by size: 1553 1202 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2506 2972 1132 Size: 1374 466 3774 Fragments arranged by size: 3774 1374 466 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2049 2787 3053 513 Size: 552 984 738 266 3074 Fragments arranged by size: 3074 984 738 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 3641 3658 3811 3996 4441 325 Size: 1255 921 1140 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1140 921 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2682 2695 2841 Size: 274 80 33 51 1443 705 13 146 Cuts at: 2841 3860 5572 96 Size: 1019 1712 138 Fragments arranged by size: 1712 1443 1019 705 274 146 138 80 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5574 5606 5574 Size: 32 5582 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2655 2939 1870 Size: 785 284 4545 Fragments arranged by size: 4545 785 284 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI