Restriction map for
pFN21AB4406
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# NO CUT ENZYMES ( 56 enzymes) #
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AclI AflII AgeI AhdI AscI
AspEI AspI AviII AvrII BbeI
BfrI BlnI BsiEI BsiWI BsmBI
BspDI Bst1107I BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
FseI FspI HpaI KasI KspI
MluI NarI NotI PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Alw44I 319 GTGCAC G^TGCAC
ApaI 4243 GGGCCC GGGCC^C
ApaLI 319 GTGCAC G^TGCAC
BamHI 3690 GGATCC G^GATCC
BbrPI 4251 CACGTG CAC^GTG
BbvCI 2523 CCTCAGC CCTCAGC(-5/-2)
BsaBI 3685 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 725 TCATGA T^CATGA
BstBI 4426 TTCGAA TT^CGAA
BstEII 1898 GGTCACC G^GT[ACGT]ACC
BstPI 1898 GGTCACC G^GT[ACGT]ACC
DrdI 281 GACAGGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1898 GGTCACC G^GT[ACGT]ACC
EcoT22I 6371 ATGCAT ATGCA^T
MamI 3685 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1189 GCCGGC GCC^GGC
NdeI 4794 CATATG CA^TATG
NgoMIV 1189 GCCGGC G^CCGGC
NheI 1543 GCTAGC G^CTAGC
NruI 28 TCGCGA TCG^CGA
NsiI 6371 ATGCAT ATGCA^T
NspV 4426 TTCGAA TT^CGAA
PmaCI 4251 CACGTG CAC^GTG
PmlI 4251 CACGTG CAC^GTG
RcaI 725 TCATGA T^CATGA
SacI 74 GAGCTC GAGCT^C
SfuI 4426 TTCGAA TT^CGAA
SpeI 5179 ACTAGT A^CTAGT
SspI 1043 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AatII 2428 GACGTC GACGT^C
6622 GACGTC
Acc65I 4319 GGTACC G^GTACC
5736 GGTACC
AccI 1267 GTCTAC GT^[AC][GT]AC
4768 GTCTAC
AcyI 2428 GACGTC G[GA]^CG[CT]C
6622 GACGTC
AocI 3181 CCTCAGG CC^T[ACGT]AGG
4929 CCTGAGG
AseI 1036 ATTAAT AT^TAAT
1285 ATTAAT
AsnI 1036 ATTAAT AT^TAAT
1285 ATTAAT
Asp718I 4319 GGTACC G^GTACC
5736 GGTACC
BcgI 3918 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
341 GCAACGTGATCG
BciVI 3660 GGATAC GTATCC(6/5)
3392 GTATCC
BclI 2474 TGATCA T^GATCA
6108 TGATCA
BsaHI 2428 GACGTC G[GA]^CG[CT]C
6622 GACGTC
BsmI 1472 GAATGC GAATGC(1/-1)
4429 GAATGC
BssHII 116 GCGCGC G^CGCGC
184 GCGCGC
BssSI 145 CTCGTG CACGAG(-5/-1)
2647 CTCGTG
BstAPI 96 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6076 GCACCTGATGC
Bsu36I 3181 CCTCAGG CC^T[ACGT]AGG
4929 CCTGAGG
DraI 6141 TTTAAA TTT^AAA
6244 TTTAAA
DraIII 1243 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2740 CACCTGGTG
Eco81I 3181 CCTCAGG CC^T[ACGT]AGG
4929 CCTGAGG
EcoRV 5254 GATATC GAT^ATC
5314 GATATC
FbaI 2474 TGATCA T^GATCA
6108 TGATCA
HgaI 93 GACGC GACGC(5/10)
541 GACGC
Hin1I 2428 GACGTC G[GA]^CG[CT]C
6622 GACGTC
HindIII 2504 AAGCTT A^AGCTT
5289 AAGCTT
KpnI 4319 GGTACC GGTAC^C
5736 GGTACC
PshBI 1036 ATTAAT AT^TAAT
1285 ATTAAT
SapI 1776 GAAGAGC GCTCTTC(1/4)
5655 GAAGAGC
SexAI 1070 ACCAGGT A^CC[AT]GGT
2741 ACCTGGT
SmaI 857 CCCGGG CCC^GGG
2033 CCCGGG
XmaCI 857 CCCGGG C^CCGGG
2033 CCCGGG
XmaI 857 CCCGGG C^CCGGG
2033 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
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AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI