(Circular) (Six-base) MAPSORT of: pFN21AB4343.seq Check: 3546 from: 1 to: 5797 pFN21AB4343 5797 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 3140 1937 Size: 667 536 4594 Fragments arranged by size: 4594 667 536 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2445 2712 3860 5244 5512 1632 Size: 813 267 1148 1384 268 1917 Fragments arranged by size: 1917 1384 1148 813 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2749 1166 Size: 1583 4214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 2029 3540 5112 2029 Size: 1511 1572 2714 Fragments arranged by size: 2714 1572 1511 ApaI G_GGCC'C Cuts at: 2014 2014 Size: 5797 ApaLI G'TGCA_C Cuts at: 2664 3004 3854 5010 2664 Size: 340 850 1156 3451 Fragments arranged by size: 3451 1156 850 340 ApoI r'AATT_y Cuts at: 1493 2610 3060 3106 3332 3484 5593 1493 Size: 1117 450 46 226 152 2109 1697 Fragments arranged by size: 2109 1697 1117 450 226 152 46 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 2014 3045 3116 1391 Size: 623 1031 71 4072 Fragments arranged by size: 4072 1031 623 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4826 BbvCI CC'TCA_GC Cuts at: 2294 2294 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4408 136 Size: 107 122 71 631 605 337 2399 1525 Fragments arranged by size: 2399 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2014 2668 3008 3858 5014 1790 Size: 224 654 340 850 1156 2573 Fragments arranged by size: 2573 1156 850 654 340 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 3528 4486 Size: 416 53 83 399 24 622 1489 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1489 958 622 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4457 1239 Size: 3218 2579 Bpu10I CC'TnA_GC Cuts at: 2294 2294 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2186 3922 4787 5085 2186 Size: 1736 865 298 2898 Fragments arranged by size: 2898 1736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2224 2515 4460 882 Size: 617 725 291 1945 2219 Fragments arranged by size: 2219 1945 725 617 291 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 2409 5791 2409 Size: 3382 2415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2454 3604 3986 4621 Size: 53 83 186 936 921 1150 382 635 Cuts at: 4621 275 Size: 1451 Fragments arranged by size: 1451 1150 936 921 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2981 3011 1178 Size: 30 165 30 1578 30 3964 Fragments arranged by size: 3964 1578 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5797 BseYI C'CCAG_C Cuts at: 5000 5496 5000 Size: 496 5301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2773 2941 3109 1163 Size: 573 1037 168 168 3851 Fragments arranged by size: 3851 1037 573 168 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2668 3008 3045 3858 3943 5014 2668 Size: 340 37 813 85 1071 3451 Fragments arranged by size: 3451 1071 813 340 85 37 BsmI GAATG_Cn' Cuts at: 2590 3308 3401 2590 Size: 718 93 4986 Fragments arranged by size: 4986 718 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2668 3008 3045 3116 3858 3943 Size: 399 224 654 340 37 71 742 85 Cuts at: 3943 5014 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 654 399 340 224 85 71 37 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 3152 844 Size: 1417 891 3489 Fragments arranged by size: 3489 1417 891 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstYI r'GATC_y Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792 Size: 1155 367 385 17 1452 86 12 346 Cuts at: 5792 1972 Size: 1977 Fragments arranged by size: 1977 1452 1155 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2015 513 Size: 552 950 4295 Fragments arranged by size: 4295 950 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 3012 3012 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762 Size: 54 1425 50 275 18 1414 889 1629 Cuts at: 5762 8 Size: 43 Fragments arranged by size: 1629 1425 1414 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2147 3727 1353 Size: 723 71 1580 3423 Fragments arranged by size: 3423 1580 723 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3043 3043 Size: 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2445 2712 3860 4457 5244 5512 1239 Size: 393 813 267 1148 597 787 268 1524 Fragments arranged by size: 1524 1148 813 787 597 393 268 267 EcoNI CCTnn'n_nnAGG Cuts at: 2325 2325 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2011 2011 Size: 5797 EcoRI G'AATT_C Cuts at: 3060 3106 3484 3060 Size: 46 378 5373 Fragments arranged by size: 5373 378 46 EcoRV GAT'ATC Cuts at: 2096 2096 Size: 5797 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2508 2508 Size: 5797 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373 Size: 32 135 32 2866 32 42 32 2626 Fragments arranged by size: 2866 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2749 3755 4114 4273 518 Size: 648 1583 1006 359 159 2042 Fragments arranged by size: 2042 1583 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 3213 3890 5038 5283 1606 Size: 411 1196 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 677 411 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 2076 2633 1348 Size: 728 557 4512 Fragments arranged by size: 4512 728 557 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2639 1108 Size: 1531 4266 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2010 2010 Size: 5797 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 SacI G_AGCT'C Cuts at: 3045 3045 Size: 5797 SacII CC_GC'GG Cuts at: 2018 2018 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 3145 4280 4961 5152 826 Size: 219 2100 1135 681 191 1471 Fragments arranged by size: 2100 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3901 4802 5064 820 Size: 197 926 222 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 2556 3721 5 Size: 47 2504 1165 2081 Fragments arranged by size: 2504 2081 1165 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2058 3236 513 Size: 552 993 1178 3074 Fragments arranged by size: 3074 1178 993 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2370 3824 3841 3994 4179 4624 325 Size: 1255 790 1454 17 153 185 445 1498 Fragments arranged by size: 1498 1454 1255 790 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2474 2891 4043 Size: 274 80 33 51 1443 497 417 1152 Cuts at: 4043 5755 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1152 497 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2535 2535 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI