(Circular) (Six-base) MAPSORT of: pFN21AB4280.seq Check: 1173 from: 1 to: 5518 pFN21AB4280 5518 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2592 2839 2592 Size: 247 5271 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 3581 4965 5233 1632 Size: 920 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1029 920 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 2698 4417 5394 1203 Size: 1495 1719 977 1327 Fragments arranged by size: 1719 1495 1327 977 AgeI A'CCGG_T Cuts at: 2589 4349 2589 Size: 1760 3758 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 2495 1166 Size: 1329 4189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 3261 4833 3261 Size: 1572 3946 ApaI G_GGCC'C Cuts at: 2798 2817 2798 Size: 19 5499 ApaLI G'TGCA_C Cuts at: 2725 3575 4731 2725 Size: 850 1156 3512 Fragments arranged by size: 3512 1156 850 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2210 2843 1870 Size: 73 267 633 4545 Fragments arranged by size: 4545 633 267 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2592 2839 4294 618 Size: 325 217 627 805 247 1455 1842 Fragments arranged by size: 1842 1455 805 627 325 247 217 BanII G_rGCy'C Cuts at: 1391 2211 2798 2817 2837 1391 Size: 820 587 19 20 4072 Fragments arranged by size: 4072 820 587 20 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2560 928 Size: 971 661 3886 Fragments arranged by size: 3886 971 661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3709 3743 1903 Size: 34 50 34 1688 34 3678 Fragments arranged by size: 3678 1688 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 4129 136 Size: 107 122 71 631 605 440 2017 1525 Fragments arranged by size: 2017 1525 631 605 440 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 2729 2798 2817 3579 4735 1790 Size: 939 69 19 762 1156 2573 Fragments arranged by size: 2573 1156 939 762 69 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 3249 4207 Size: 416 53 83 399 24 681 1151 958 Cuts at: 4207 442 Size: 1753 Fragments arranged by size: 1753 1151 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2363 2795 4178 1239 Size: 1013 111 432 1383 2579 Fragments arranged by size: 2579 1383 1013 432 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 882 Size: 617 2682 2219 Fragments arranged by size: 2682 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2701 3309 493 Size: 711 1497 608 2702 Fragments arranged by size: 2702 1497 711 608 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2307 3325 3707 4342 Size: 53 83 186 936 774 1018 382 635 Cuts at: 4342 275 Size: 1451 Fragments arranged by size: 1451 1018 936 774 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 3951 4349 4623 4770 5181 2589 Size: 1362 398 274 147 411 2926 Fragments arranged by size: 2926 1362 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4351 Fragments arranged by size: 4351 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5518 BseYI C'CCAG_C Cuts at: 2044 2094 2347 2451 2526 4721 5217 2044 Size: 50 253 104 75 2195 496 2345 Fragments arranged by size: 2345 2195 496 253 104 75 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4945 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2729 3579 3664 4735 2325 Size: 404 850 85 1071 3108 Fragments arranged by size: 3108 1071 850 404 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2325 2729 2798 2817 2837 3579 Size: 399 421 114 404 69 19 20 742 Cuts at: 3579 3664 4735 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 404 399 114 85 69 20 19 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 2604 2873 844 Size: 1313 447 269 3489 Fragments arranged by size: 3489 1313 447 269 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2589 4162 4349 1700 Size: 110 779 1573 187 2869 Fragments arranged by size: 2869 1573 779 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 2488 1184 Size: 1304 4214 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2278 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513 Size: 876 367 385 17 1452 86 12 346 Cuts at: 5513 1972 Size: 1977 Fragments arranged by size: 1977 1452 876 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4966 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3828 3848 3034 Size: 794 20 4704 Fragments arranged by size: 4704 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483 Size: 54 1425 50 275 18 1135 889 1629 Cuts at: 5483 8 Size: 43 Fragments arranged by size: 1629 1425 1135 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3448 1353 Size: 2095 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2363 2552 2795 3581 4178 4965 Size: 393 620 111 189 243 786 597 787 Cuts at: 4965 5233 1239 Size: 268 1524 Fragments arranged by size: 1524 787 786 620 597 393 268 243 189 111 EcoNI CCTnn'n_nnAGG Cuts at: 2777 2777 Size: 5518 EcoO109I rG'GnC_Cy Cuts at: 2014 2098 2352 2794 2813 2014 Size: 84 254 442 19 4719 Fragments arranged by size: 4719 442 254 84 19 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 2378 4024 2378 Size: 1646 3872 FspAI rTGC'GCAy Cuts at: 2378 2378 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373 Size: 32 135 32 2587 32 42 32 2626 Fragments arranged by size: 2626 2587 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2596 2843 2596 Size: 247 5271 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2482 4632 4816 1305 Size: 1177 2150 184 2007 Fragments arranged by size: 2150 2007 1177 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2495 3476 3835 3994 518 Size: 648 1110 219 981 359 159 2042 Fragments arranged by size: 2042 1110 981 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2036 2372 2455 2530 2715 2934 3611 4759 Size: 430 336 83 75 185 219 677 1148 Cuts at: 4759 5004 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 430 336 245 219 185 83 75 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4966 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2204 2876 4421 1132 Size: 97 975 672 1545 2229 Fragments arranged by size: 2229 1545 975 672 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2458 1108 Size: 1350 4168 PfoI T'CCnGG_A Cuts at: 2745 2745 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PmlI CAC'GTG Cuts at: 2701 2701 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PspOMI G'GGCC_C Cuts at: 2794 2813 2794 Size: 19 5499 PstI C_TGCA'G Cuts at: 830 2136 2199 2870 830 Size: 1306 63 671 3478 Fragments arranged by size: 3478 1306 671 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2036 2372 2530 2715 2036 Size: 336 158 185 4839 Fragments arranged by size: 4839 336 185 158 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2132 2195 2866 4001 4682 4873 826 Size: 219 1087 63 671 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 671 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2212 2845 1872 Size: 340 633 4545 Fragments arranged by size: 4545 633 340 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2204 2876 1132 Size: 1072 672 3774 Fragments arranged by size: 3774 1072 672 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2166 2957 513 Size: 552 1101 791 3074 Fragments arranged by size: 3074 1101 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 2465 3545 3562 3715 3900 4345 Size: 1255 567 318 1080 17 153 185 445 Cuts at: 4345 325 Size: 1498 Fragments arranged by size: 1498 1255 1080 567 445 318 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 5478 5510 2315 Size: 32 3131 32 2323 Fragments arranged by size: 3131 2323 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2486 2564 1316 Size: 1170 78 4270 Fragments arranged by size: 4270 1170 78 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2210 2843 1870 Size: 340 633 4545 Fragments arranged by size: 4545 633 340 XmnI GAAnn'nnTTC Cuts at: 2123 3647 2123 Size: 1524 3994 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI