(Circular) (Six-base) MAPSORT of: pFN21AB4216.seq Check: 437 from: 1 to: 8128 pFN21AB4216 8128 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 5283 2599 Size: 2684 5444 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4640 5938 278 Size: 53 83 186 936 3104 1298 2468 Fragments arranged by size: 3104 2468 1298 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3435 5471 1937 Size: 1498 2036 4594 Fragments arranged by size: 4594 2036 1498 Acc65I G'GTAC_C Cuts at: 2950 5449 2950 Size: 2499 5629 AclI AA'CG_TT Cuts at: 1563 6255 6628 1563 Size: 4692 373 3063 Fragments arranged by size: 4692 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2247 2381 2531 2798 3206 3281 6191 7575 Size: 615 134 150 267 408 75 2910 1384 Cuts at: 7575 7843 1632 Size: 268 1917 Fragments arranged by size: 2910 1917 1384 615 408 268 267 150 134 75 AfeI AGC'GCT Cuts at: 4959 4959 Size: 8128 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7931 AflIII A'CryG_T Cuts at: 1203 5010 7027 8004 1203 Size: 3807 2017 977 1327 Fragments arranged by size: 3807 2017 1327 977 AgeI A'CCGG_T Cuts at: 6959 6959 Size: 8128 AhdI GACnn_n'nnGTC Cuts at: 3319 6857 3319 Size: 3538 4590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2688 2712 4439 4799 1184 Size: 1504 24 1727 360 4513 Fragments arranged by size: 4513 1727 1504 360 24 AlwNI CAG_nnn'CTG Cuts at: 2699 3311 3554 3587 3782 3956 4240 5021 5164 Size: 612 243 33 195 174 284 781 143 Cuts at: 5164 5871 7443 2699 Size: 707 1572 3384 Fragments arranged by size: 3384 1572 781 707 612 284 243 195 174 143 33 ApaI G_GGCC'C Cuts at: 3523 3523 Size: 8128 ApaLI G'TGCA_C Cuts at: 6185 7341 6185 Size: 1156 6972 ApoI r'AATT_y Cuts at: 1493 4502 5437 5663 5815 7924 1493 Size: 3009 935 226 152 2109 1697 Fragments arranged by size: 3009 2109 1697 935 226 152 AseI AT'TA_AT Cuts at: 160 1032 6682 160 Size: 872 5650 1606 Fragments arranged by size: 5650 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8128 AvaI C'yCGr_G Cuts at: 1870 1943 3993 4250 5299 5453 1870 Size: 73 2050 257 1049 154 4545 Fragments arranged by size: 4545 2050 1049 257 154 73 AvrII C'CTAG_G Cuts at: 3229 3229 Size: 8128 BamHI G'GATC_C Cuts at: 4058 5458 5825 4058 Size: 1400 367 6361 Fragments arranged by size: 6361 1400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 3732 5449 6904 618 Size: 325 217 627 1163 782 1717 1455 1842 Fragments arranged by size: 1842 1717 1455 1163 782 627 325 217 BanII G_rGCy'C Cuts at: 1391 2979 3523 4176 4234 4571 4589 4748 5447 Size: 1588 544 653 58 337 18 159 699 Cuts at: 5447 1391 Size: 4072 Fragments arranged by size: 4072 1588 699 653 544 337 159 58 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2786 3919 3978 928 Size: 971 887 1133 59 5078 Fragments arranged by size: 5078 1133 971 887 59 BbvCI CC'TCA_GC Cuts at: 4223 4223 Size: 8128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4241 4275 6319 6353 1903 Size: 34 2304 34 2044 34 3678 Fragments arranged by size: 3678 2304 2044 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6020 7230 6020 Size: 1210 6918 BclI T'GATC_A Cuts at: 1548 2457 1548 Size: 909 7219 BfrBI ATG'CAT Cuts at: 4786 4786 Size: 8128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3959 4245 4988 Size: 107 122 71 631 605 2287 286 743 Cuts at: 4988 6739 136 Size: 1751 1525 Fragments arranged by size: 2287 1751 1525 743 631 605 286 122 107 71 BglII A'GATC_T Cuts at: 3748 8123 3748 Size: 4375 3753 BlpI GC'TnA_GC Cuts at: 5545 5545 Size: 8128 Bme1580I G_kGCm'C Cuts at: 1790 2940 3523 3826 4369 6189 7345 1790 Size: 1150 583 303 543 1820 1156 2573 Fragments arranged by size: 2573 1820 1156 1150 583 543 303 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5859 6817 442 Size: 416 53 83 399 24 4442 958 1753 Fragments arranged by size: 4442 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8128 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3424 3424 Size: 8128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 3748 4448 4492 4497 4538 4564 4652 Size: 815 1694 700 44 5 41 26 88 Cuts at: 4652 5363 6788 1239 Size: 711 1425 2579 Fragments arranged by size: 2579 1694 1425 815 711 700 88 44 41 26 5 Bpu10I CC'TnA_GC Cuts at: 3653 4223 3653 Size: 570 7558 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2538 2993 3902 4160 4580 6253 7118 7416 2538 Size: 455 909 258 420 1673 865 298 3250 Fragments arranged by size: 3250 1673 909 865 455 420 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2039 4473 4994 6791 882 Size: 617 531 9 2434 521 1797 2219 Fragments arranged by size: 2434 2219 1797 617 531 521 9 BsaAI yAC'GTr Cuts at: 493 1204 4912 5919 493 Size: 711 3708 1007 2702 Fragments arranged by size: 3708 2702 1007 711 BsaBI GATnn'nnATC Cuts at: 3753 8122 3753 Size: 4369 3759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3087 4637 5935 6317 Size: 53 83 186 936 1554 1550 1298 382 Cuts at: 6317 6952 275 Size: 635 1451 Fragments arranged by size: 1554 1550 1451 1298 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3735 6561 6959 7233 7380 7791 3735 Size: 2826 398 274 147 411 4072 Fragments arranged by size: 4072 2826 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3695 3725 5348 5378 1178 Size: 30 165 30 2292 30 1623 30 3928 Fragments arranged by size: 3928 2292 1623 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3025 3402 3703 3780 4133 5284 1186 Size: 1839 377 301 77 353 1151 4030 Fragments arranged by size: 4030 1839 1151 377 353 301 77 BseYI C'CCAG_C Cuts at: 2023 2059 2409 2415 2628 3304 3424 4198 4378 Size: 36 350 6 213 676 120 774 180 Cuts at: 4378 5083 5398 7331 7827 2023 Size: 705 315 1933 496 2324 Fragments arranged by size: 2324 1933 774 705 676 496 350 315 213 180 120 36 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2633 2708 2732 4007 4016 4134 1163 Size: 573 897 75 24 1275 9 118 5157 Fragments arranged by size: 5157 1275 897 573 118 75 24 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5359 5578 6339 7367 1540 Size: 275 186 3358 219 761 1028 2301 Fragments arranged by size: 3358 2301 1028 761 275 219 186 BsiHKAI G_wGCw'C Cuts at: 4139 4257 4516 4589 4596 5201 6189 6274 7345 Size: 118 259 73 7 605 988 85 1071 Cuts at: 7345 4139 Size: 4922 Fragments arranged by size: 4922 1071 988 605 259 118 85 73 7 BsmI GAATG_Cn' Cuts at: 2527 5639 5732 2527 Size: 3112 93 4923 Fragments arranged by size: 4923 3112 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4668 4668 Size: 8128 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2940 2979 3523 3826 4139 4176 4234 Size: 399 1150 39 544 303 313 37 58 Cuts at: 4234 4257 4369 4516 4571 4589 4596 4748 5201 Size: 23 112 147 55 18 7 152 453 Cuts at: 5201 5447 6189 6274 7345 1391 Size: 246 742 85 1071 2174 Fragments arranged by size: 2174 1150 1071 742 544 453 399 313 303 246 152 147 112 85 58 55 39 37 23 18 7 BspEI T'CCGG_A Cuts at: 7791 7791 Size: 8128 BspHI T'CATG_A Cuts at: 6017 7747 6017 Size: 1730 6398 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 2707 2938 3139 3809 4387 4431 4944 Size: 1755 108 231 201 670 578 44 513 Cuts at: 4944 5283 5483 844 Size: 339 200 3489 Fragments arranged by size: 3489 1755 670 578 513 339 231 201 200 108 44 BsrBI CCG'CTC Cuts at: 1394 1823 3441 4321 5242 6015 1394 Size: 429 1618 880 921 773 3507 Fragments arranged by size: 3507 1618 921 880 773 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2233 4389 4834 5415 6623 6797 65 Size: 1183 985 2156 445 581 1208 174 1396 Fragments arranged by size: 2156 1396 1208 1183 985 581 445 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2590 3785 3936 4017 5358 6772 6959 Size: 110 780 1195 151 81 1341 1414 187 Cuts at: 6959 1700 Size: 2869 Fragments arranged by size: 2869 1414 1341 1195 780 187 151 110 81 BsrGI T'GTAC_A Cuts at: 96 4075 96 Size: 3979 4149 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8128 BssSI C'ACGA_G Cuts at: 6188 7200 6188 Size: 1012 7116 BstAPI GCAn_nnn'nTGC Cuts at: 794 2611 3782 4030 5376 794 Size: 1817 1171 248 1346 3546 Fragments arranged by size: 3546 1817 1346 1171 248 BstBI TT'CG_AA Cuts at: 6932 6932 Size: 8128 BstEII G'GTnAC_C Cuts at: 1184 2593 3718 1184 Size: 1409 1125 5594 Fragments arranged by size: 5594 1409 1125 BstXI CCAn_nnnn'nTGG Cuts at: 4516 4729 5260 4516 Size: 213 531 7384 Fragments arranged by size: 7384 531 213 BstYI r'GATC_y Cuts at: 1972 3748 3754 4058 4125 4149 5458 5825 6210 Size: 1776 6 304 67 24 1309 367 385 Cuts at: 6210 6227 7679 7765 7777 8123 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1776 1452 1309 385 367 346 304 86 67 24 17 12 6 Bsu36I CC'TnA_GG Cuts at: 4458 4458 Size: 8128 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 4167 513 Size: 552 939 36 2127 4474 Fragments arranged by size: 4474 2127 939 552 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7389 Fragments arranged by size: 7389 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2209 2893 3515 3568 3824 5319 5409 5644 Size: 30 684 622 53 256 1495 90 235 Cuts at: 5644 6438 6458 2179 Size: 794 20 3849 Fragments arranged by size: 3849 1495 794 684 622 256 235 90 53 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8128 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2859 2894 4632 4667 4861 4896 529 Size: 35 2295 35 1738 35 194 35 3761 Fragments arranged by size: 3761 2295 1738 194 35 35 35 35 DraI TTT'AAA Cuts at: 5432 5432 Size: 8128 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7135 809 Size: 507 5819 1802 Fragments arranged by size: 5819 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2649 3556 3698 Size: 54 1425 50 275 18 819 907 142 Cuts at: 3698 4209 4653 4852 5230 5322 5575 6464 8093 Size: 511 444 199 378 92 253 889 1629 Cuts at: 8093 8 Size: 43 Fragments arranged by size: 1629 1425 907 889 819 511 444 378 275 253 199 142 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5575 1537 Size: 275 3763 4090 Fragments arranged by size: 4090 3763 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3624 4485 6058 1353 Size: 2271 861 1573 3423 Fragments arranged by size: 3423 2271 1573 861 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3013 4051 4591 5069 6715 7089 Size: 1064 138 1424 1038 540 478 1646 374 Cuts at: 7089 7235 387 Size: 146 1280 Fragments arranged by size: 1646 1424 1280 1064 1038 540 478 374 146 138 EcoICRI GAG'CTC Cuts at: 4587 4587 Size: 8128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2247 2381 2531 2798 3206 3281 Size: 393 422 193 134 150 267 408 75 Cuts at: 3281 3748 4448 4492 4497 4538 4564 4652 5363 Size: 467 700 44 5 41 26 88 711 Cuts at: 5363 6191 6788 7575 7843 1239 Size: 828 597 787 268 1524 Fragments arranged by size: 1524 828 787 711 700 597 467 422 408 393 268 267 193 150 134 88 75 44 41 26 5 EcoNI CCTnn'n_nnAGG Cuts at: 3727 4754 5345 3727 Size: 1027 591 6510 Fragments arranged by size: 6510 1027 591 EcoO109I rG'GnC_Cy Cuts at: 2719 3321 3519 4726 5044 2719 Size: 602 198 1207 318 5803 Fragments arranged by size: 5803 1207 602 318 198 EcoRI G'AATT_C Cuts at: 4502 5437 5815 4502 Size: 935 378 6815 Fragments arranged by size: 6815 935 378 EcoRV GAT'ATC Cuts at: 3029 3029 Size: 8128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3943 4219 4780 3943 Size: 276 561 7291 Fragments arranged by size: 7291 561 276 FspI TGC'GCA Cuts at: 4395 5060 6634 4395 Size: 665 1574 5889 Fragments arranged by size: 5889 1574 665 HaeII r_GCGC'y Cuts at: 1678 3425 3993 4961 5022 5365 7275 1678 Size: 1747 568 968 61 343 1910 2531 Fragments arranged by size: 2531 1910 1747 968 568 343 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3305 3337 3601 3633 5348 Size: 32 135 32 1733 32 264 32 1715 Cuts at: 5348 5380 6769 6801 6843 6875 1373 Size: 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1733 1715 1389 264 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5472 5718 669 Size: 988 281 3534 246 3079 Fragments arranged by size: 3534 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3209 4046 748 Size: 2461 837 4830 Fragments arranged by size: 4830 2461 837 HpaI GTT'AAC Cuts at: 5718 5718 Size: 8128 KpnI G_GTAC'C Cuts at: 2954 5453 2954 Size: 2499 5629 MfeI C'AATT_G Cuts at: 5727 5727 Size: 8128 MluI A'CGCG_T Cuts at: 5010 8004 5010 Size: 2994 5134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2357 5062 7242 7426 1305 Size: 1052 2705 2180 184 2007 Fragments arranged by size: 2705 2180 2007 1052 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4211 4655 4854 10 Size: 54 1425 343 2379 444 199 3284 Fragments arranged by size: 3284 2379 1425 444 343 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 6086 6445 6604 518 Size: 648 4920 359 159 2042 Fragments arranged by size: 4920 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2016 2152 2196 2247 2437 2632 3779 4030 Size: 410 136 44 51 190 195 1147 251 Cuts at: 4030 4343 4382 4662 4685 4921 5161 5308 5544 Size: 313 39 280 23 236 240 147 236 Cuts at: 5544 6221 7369 7614 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1147 677 410 313 280 251 245 240 236 236 195 190 147 136 51 44 39 23 NaeI GCC'GGC Cuts at: 3787 3938 3787 Size: 151 7977 NcoI C'CATG_G Cuts at: 513 1065 2004 2040 4167 513 Size: 552 939 36 2127 4474 Fragments arranged by size: 4474 2127 939 552 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 8128 NgoMIV G'CCGG_C Cuts at: 3785 3936 3785 Size: 151 7977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8128 NotI GC'GGCC_GC Cuts at: 5575 5575 Size: 8128 NsiI A_TGCA'T Cuts at: 4788 4788 Size: 8128 NspI r_CATG'y Cuts at: 1132 1229 4790 5486 7031 1132 Size: 97 3561 696 1545 2229 Fragments arranged by size: 3561 2229 1545 696 97 PasI CC'CwG_GG Cuts at: 1348 2077 2379 4297 4606 4799 1348 Size: 729 302 1918 309 193 4677 Fragments arranged by size: 4677 1918 729 309 302 193 PciI A'CATG_T Cuts at: 7027 7027 Size: 8128 PflMI CCAn_nnn'nTGG Cuts at: 1108 2566 4297 4662 4993 5164 1108 Size: 1458 1731 365 331 171 4072 Fragments arranged by size: 4072 1731 1458 365 331 171 PfoI T'CCnGG_A Cuts at: 3863 4580 3863 Size: 717 7411 PmeI GTTT'AAAC Cuts at: 5432 5432 Size: 8128 PmlI CAC'GTG Cuts at: 4912 4912 Size: 8128 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6197 6229 7710 7742 6197 Size: 32 1481 32 6583 Fragments arranged by size: 6583 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2719 3321 5044 2719 Size: 602 1723 5803 Fragments arranged by size: 5803 1723 602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8128 PsiI TTA'TAA Cuts at: 5698 5698 Size: 8128 PspOMI G'GGCC_C Cuts at: 3519 3519 Size: 8128 PstI C_TGCA'G Cuts at: 830 2667 3510 3857 3891 4057 4778 5480 830 Size: 1837 843 347 34 166 721 702 3478 Fragments arranged by size: 3478 1837 843 721 702 347 166 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8128 PvuII CAG'CTG Cuts at: 2152 2437 3779 4030 4343 4382 4662 4685 4921 Size: 285 1342 251 313 39 280 23 236 Cuts at: 4921 5161 2152 Size: 240 5119 Fragments arranged by size: 5119 1342 313 285 280 251 240 236 39 23 SacI G_AGCT'C Cuts at: 4589 4589 Size: 8128 SalI G'TCGA_C Cuts at: 1936 5470 1936 Size: 3534 4594 SanDI GG'GwC_CC Cuts at: 3321 3321 Size: 8128 SbfI CC_TGCA'GG Cuts at: 4057 5480 4057 Size: 1423 6705 ScaI AGT'ACT Cuts at: 4523 6376 8088 4523 Size: 1853 1712 4563 Fragments arranged by size: 4563 1853 1712 SexAI A'CCwGG_T Cuts at: 3069 3069 Size: 8128 SfcI C'TryA_G Cuts at: 826 1045 2663 3506 3853 3880 3887 4053 4437 Size: 219 1618 843 347 27 7 166 384 Cuts at: 4437 4774 5476 6611 7292 7483 826 Size: 337 702 1135 681 191 1471 Fragments arranged by size: 1618 1471 1135 843 702 681 384 347 337 219 191 166 27 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8128 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8128 SmaI CCC'GGG Cuts at: 1872 3995 5301 5455 1872 Size: 2123 1306 154 4545 Fragments arranged by size: 4545 2123 1306 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 2553 2972 3917 4175 4595 6232 Size: 197 926 610 419 945 258 420 1637 Cuts at: 6232 7133 7395 820 Size: 901 262 1553 Fragments arranged by size: 1637 1553 945 926 901 610 420 419 262 258 197 SnaBI TAC'GTA Cuts at: 493 5919 493 Size: 5426 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8128 SphI G_CATG'C Cuts at: 1132 4790 5486 1132 Size: 3658 696 3774 Fragments arranged by size: 3774 3658 696 SspI AAT'ATT Cuts at: 5 52 6052 5 Size: 47 6000 2081 Fragments arranged by size: 6000 2081 47 StuI AGG'CCT Cuts at: 1517 4409 1517 Size: 2892 5236 StyI C'CwwG_G Cuts at: 513 1065 2004 2040 3229 4167 4347 4446 4722 Size: 552 939 36 1189 938 180 99 276 Cuts at: 4722 5567 513 Size: 845 3074 Fragments arranged by size: 3074 1189 939 938 845 552 276 180 99 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2015 2297 2392 3555 3706 5168 5390 Size: 1255 435 282 95 1163 151 1462 222 Cuts at: 5390 6155 6172 6325 6510 6955 325 Size: 765 17 153 185 445 1498 Fragments arranged by size: 1498 1462 1255 1163 765 445 435 282 222 185 153 151 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 4075 4521 Size: 274 80 33 51 1443 643 1455 446 Cuts at: 4521 6374 8086 96 Size: 1853 1712 138 Fragments arranged by size: 1853 1712 1455 1443 643 446 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2350 2382 3695 3727 5061 5093 8088 8120 2350 Size: 32 1313 32 1334 32 2995 32 2358 Fragments arranged by size: 2995 2358 1334 1313 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3071 1316 Size: 1755 6373 XbaI T'CTAG_A Cuts at: 5464 5464 Size: 8128 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3838 4241 4990 3838 Size: 403 749 6976 Fragments arranged by size: 6976 749 403 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8128 XmaI C'CCGG_G Cuts at: 1870 3993 5299 5453 1870 Size: 2123 1306 154 4545 Fragments arranged by size: 4545 2123 1306 154 XmnI GAAnn'nnTTC Cuts at: 3926 4310 6257 3926 Size: 384 1947 5797 Fragments arranged by size: 5797 1947 384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4638 5936 276 Size: 53 83 186 936 3104 1298 2468 Fragments arranged by size: 3104 2468 1298 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI