Restriction map for
pFN21AB4216
(Linear) (Six-base) MAPSORT of: KIBB4216.seq Check: 361 from: 1 to: 3423
KIBB4216.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 3278 3423
Size: 594 2684 145
Fragments arranged by size:
2684 594 145
AatII G_ACGT'C
Cuts at: 0 2635 3423
Size: 2635 788
AccI GT'mk_AC
Cuts at: 0 1430 3423
Size: 1430 1993
Acc65I G'GTAC_C
Cuts at: 0 945 3423
Size: 945 2478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 376 526 793 1201 1276 3423
Size: 242 134 150 267 408 75 2147
Fragments arranged by size:
2147 408 267 242 150 134 75
AfeI AGC'GCT
Cuts at: 0 2954 3423
Size: 2954 469
AflIII A'CryG_T
Cuts at: 0 3005 3423
Size: 3005 418
AhdI GACnn_n'nnGTC
Cuts at: 0 1314 3423
Size: 1314 2109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 683 707 2434 2794 3423
Size: 683 24 1727 360 629
Fragments arranged by size:
1727 683 629 360 24
AlwNI CAG_nnn'CTG
Cuts at: 0 694 1306 1549 1582 1777 1951 2235 3016
Size: 694 612 243 33 195 174 284 781
Cuts at: 3016 3159 3423
Size: 143 264
Fragments arranged by size:
781 694 612 284 264 243 195 174
143 33
ApaI G_GGCC'C
Cuts at: 0 1518 3423
Size: 1518 1905
ApoI r'AATT_y
Cuts at: 0 2497 3423
Size: 2497 926
AvaI C'yCGr_G
Cuts at: 0 1988 2245 3294 3423
Size: 1988 257 1049 129
Fragments arranged by size:
1988 1049 257 129
AvrII C'CTAG_G
Cuts at: 0 1224 3423
Size: 1224 2199
BamHI G'GATC_C
Cuts at: 0 2053 3423
Size: 2053 1370
BanI G'GyrC_C
Cuts at: 0 945 1727 3423
Size: 945 782 1696
Fragments arranged by size:
1696 945 782
BanII G_rGCy'C
Cuts at: 0 974 1518 2171 2229 2566 2584 2743 3423
Size: 974 544 653 58 337 18 159 680
Fragments arranged by size:
974 680 653 544 337 159 58 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 1914 1973 3423
Size: 781 1133 59 1450
Fragments arranged by size:
1450 1133 781 59
BbvCI CC'TCA_GC
Cuts at: 0 2218 3423
Size: 2218 1205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2236 2270 3423
Size: 2236 34 1153
Fragments arranged by size:
2236 1153 34
BclI T'GATC_A
Cuts at: 0 452 3423
Size: 452 2971
BfrBI ATG'CAT
Cuts at: 0 2781 3423
Size: 2781 642
BglI GCCn_nnn'nGGC
Cuts at: 0 1954 2240 2983 3423
Size: 1954 286 743 440
Fragments arranged by size:
1954 743 440 286
BglII A'GATC_T
Cuts at: 0 1743 3423
Size: 1743 1680
Bme1580I G_kGCm'C
Cuts at: 0 935 1518 1821 2364 3423
Size: 935 583 303 543 1059
Fragments arranged by size:
1059 935 583 543 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1419 3423
Size: 1419 2004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1743 2443 2487 2492 2533 2559 2647
Size: 49 1694 700 44 5 41 26 88
Cuts at: 2647 3358 3423
Size: 711 65
Fragments arranged by size:
1694 711 700 88 65 49 44 41
26 5
Bpu10I CC'TnA_GC
Cuts at: 0 1648 2218 3423
Size: 1648 570 1205
Fragments arranged by size:
1648 1205 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 988 1897 2155 2575 3423
Size: 533 455 909 258 420 848
Fragments arranged by size:
909 848 533 455 420 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 34 2468 2989 3423
Size: 25 9 2434 521 434
Fragments arranged by size:
2434 521 434 25 9
BsaAI yAC'GTr
Cuts at: 0 2907 3423
Size: 2907 516
BsaBI GATnn'nnATC
Cuts at: 0 1748 3423
Size: 1748 1675
BsaHI Gr'CG_yC
Cuts at: 0 1082 2632 3423
Size: 1082 1550 791
Fragments arranged by size:
1550 1082 791
BsaWI w'CCGG_w
Cuts at: 0 1730 3423
Size: 1730 1693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1690 1720 3343 3373 3423
Size: 1690 30 1623 30 50
Fragments arranged by size:
1690 1623 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1020 1397 1698 1775 2128 3279 3423
Size: 1020 377 301 77 353 1151 144
Fragments arranged by size:
1151 1020 377 353 301 144 77
BseYI C'CCAG_C
Cuts at: 0 18 54 404 410 623 1299 1419 2193
Size: 18 36 350 6 213 676 120 774
Cuts at: 2193 2373 3078 3393 3423
Size: 180 705 315 30
Fragments arranged by size:
774 705 676 350 315 213 180 120
36 30 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 703 727 2002 2011 2129 3423
Size: 628 75 24 1275 9 118 1294
Fragments arranged by size:
1294 1275 628 118 75 24 9
BsiEI CG_ry'CG
Cuts at: 0 3354 3423
Size: 3354 69
BsiHKAI G_wGCw'C
Cuts at: 0 2134 2252 2511 2584 2591 3196 3423
Size: 2134 118 259 73 7 605 227
Fragments arranged by size:
2134 605 259 227 118 73 7
BsmI GAATG_Cn'
Cuts at: 0 522 3423
Size: 522 2901
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2663 3423
Size: 2663 760
Bsp1286I G_dGCh'C
Cuts at: 0 935 974 1518 1821 2134 2171 2229 2252
Size: 935 39 544 303 313 37 58 23
Cuts at: 2252 2364 2511 2566 2584 2591 2743 3196 3423
Size: 112 147 55 18 7 152 453 227
Fragments arranged by size:
935 544 453 313 303 227 152 147
112 58 55 39 37 23 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 702 933 1134 1804 2382 2426 2939
Size: 594 108 231 201 670 578 44 513
Cuts at: 2939 3278 3423
Size: 339 145
Fragments arranged by size:
670 594 578 513 339 231 201 145
108 44
BsrBI CCG'CTC
Cuts at: 0 1436 2316 3237 3423
Size: 1436 880 921 186
Fragments arranged by size:
1436 921 880 186
BsrDI GCAATG_nn'
Cuts at: 0 228 2384 2829 3410 3423
Size: 228 2156 445 581 13
Fragments arranged by size:
2156 581 445 228 13
BsrFI r'CCGG_y
Cuts at: 0 585 1780 1931 2012 3353 3423
Size: 585 1195 151 81 1341 70
Fragments arranged by size:
1341 1195 585 151 81 70
BsrGI T'GTAC_A
Cuts at: 0 2070 3423
Size: 2070 1353
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 1777 2025 3371 3423
Size: 606 1171 248 1346 52
Fragments arranged by size:
1346 1171 606 248 52
BstEII G'GTnAC_C
Cuts at: 0 588 1713 3423
Size: 588 1125 1710
Fragments arranged by size:
1710 1125 588
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2511 2724 3255 3423
Size: 2511 213 531 168
Fragments arranged by size:
2511 531 213 168
BstYI r'GATC_y
Cuts at: 0 1743 1749 2053 2120 2144 3423
Size: 1743 6 304 67 24 1279
Fragments arranged by size:
1743 1279 304 67 24 6
Bsu36I CC'TnA_GG
Cuts at: 0 2453 3423
Size: 2453 970
BtgI C'CryG_G
Cuts at: 0 35 2162 3423
Size: 35 2127 1261
Fragments arranged by size:
2127 1261 35
BtsI GCAGTG_nn'
Cuts at: 0 174 204 888 1510 1563 1819 3314 3404
Size: 174 30 684 622 53 256 1495 90
Cuts at: 3404 3423
Size: 19
Fragments arranged by size:
1495 684 622 256 174 90 53 30
19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 854 889 2627 2662 2856 2891 3423
Size: 854 35 1738 35 194 35 532
Fragments arranged by size:
1738 854 532 194 35 35 35
EaeI y'GGCC_r
Cuts at: 0 644 1551 1693 2204 2648 2847 3225 3317
Size: 644 907 142 511 444 199 378 92
Cuts at: 3317 3423
Size: 106
Fragments arranged by size:
907 644 511 444 378 199 142 106
92
EarI CTCTTCn'nnn_
Cuts at: 0 1619 2480 3423
Size: 1619 861 943
Fragments arranged by size:
1619 943 861
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 2046 2586 3064 3423
Size: 1008 1038 540 478 359
Fragments arranged by size:
1038 1008 540 478 359
EcoICRI GAG'CTC
Cuts at: 0 2582 3423
Size: 2582 841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 242 376 526 793 1201 1276 1743
Size: 49 193 134 150 267 408 75 467
Cuts at: 1743 2443 2487 2492 2533 2559 2647 3358 3423
Size: 700 44 5 41 26 88 711 65
Fragments arranged by size:
711 700 467 408 267 193 150 134
88 75 65 49 44 41 26 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1722 2749 3340 3423
Size: 1722 1027 591 83
Fragments arranged by size:
1722 1027 591 83
EcoO109I rG'GnC_Cy
Cuts at: 0 714 1316 1514 2721 3039 3423
Size: 714 602 198 1207 318 384
Fragments arranged by size:
1207 714 602 384 318 198
EcoRI G'AATT_C
Cuts at: 0 2497 3423
Size: 2497 926
EcoRV GAT'ATC
Cuts at: 0 1024 3423
Size: 1024 2399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1938 2214 2775 3423
Size: 1938 276 561 648
Fragments arranged by size:
1938 648 561 276
FspI TGC'GCA
Cuts at: 0 2390 3055 3423
Size: 2390 665 368
Fragments arranged by size:
2390 665 368
HaeII r_GCGC'y
Cuts at: 0 1420 1988 2956 3017 3360 3423
Size: 1420 568 968 61 343 63
Fragments arranged by size:
1420 968 568 343 63 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1596 1628 3343 3375 3423
Size: 1300 32 264 32 1715 32 48
Fragments arranged by size:
1715 1300 264 48 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1204 2041 3423
Size: 1204 837 1382
Fragments arranged by size:
1382 1204 837
KpnI G_GTAC'C
Cuts at: 0 949 3423
Size: 949 2474
MluI A'CGCG_T
Cuts at: 0 3005 3423
Size: 3005 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 3057 3423
Size: 352 2705 366
Fragments arranged by size:
2705 366 352
MscI TGG'CCA
Cuts at: 0 2206 2650 2849 3423
Size: 2206 444 199 574
Fragments arranged by size:
2206 574 444 199
MspA1I CmG'CkG
Cuts at: 0 11 147 191 242 432 627 1774 2025
Size: 11 136 44 51 190 195 1147 251
Cuts at: 2025 2338 2377 2657 2680 2916 3156 3303 3423
Size: 313 39 280 23 236 240 147 120
Fragments arranged by size:
1147 313 280 251 240 236 195 190
147 136 120 51 44 39 23 11
NaeI GCC'GGC
Cuts at: 0 1782 1933 3423
Size: 1782 151 1490
Fragments arranged by size:
1782 1490 151
NcoI C'CATG_G
Cuts at: 0 35 2162 3423
Size: 35 2127 1261
Fragments arranged by size:
2127 1261 35
NgoMIV G'CCGG_C
Cuts at: 0 1780 1931 3423
Size: 1780 151 1492
Fragments arranged by size:
1780 1492 151
NsiI A_TGCA'T
Cuts at: 0 2783 3423
Size: 2783 640
NspI r_CATG'y
Cuts at: 0 2785 3423
Size: 2785 638
PasI CC'CwG_GG
Cuts at: 0 72 374 2292 2601 2794 3423
Size: 72 302 1918 309 193 629
Fragments arranged by size:
1918 629 309 302 193 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 561 2292 2657 2988 3159 3423
Size: 561 1731 365 331 171 264
Fragments arranged by size:
1731 561 365 331 264 171
PfoI T'CCnGG_A
Cuts at: 0 1858 2575 3423
Size: 1858 717 848
Fragments arranged by size:
1858 848 717
PmlI CAC'GTG
Cuts at: 0 2907 3423
Size: 2907 516
PpuMI rG'GwC_Cy
Cuts at: 0 714 1316 3039 3423
Size: 714 602 1723 384
Fragments arranged by size:
1723 714 602 384
PspOMI G'GGCC_C
Cuts at: 0 1514 3423
Size: 1514 1909
PstI C_TGCA'G
Cuts at: 0 662 1505 1852 1886 2052 2773 3423
Size: 662 843 347 34 166 721 650
Fragments arranged by size:
843 721 662 650 347 166 34
PvuII CAG'CTG
Cuts at: 0 147 432 1774 2025 2338 2377 2657 2680
Size: 147 285 1342 251 313 39 280 23
Cuts at: 2680 2916 3156 3423
Size: 236 240 267
Fragments arranged by size:
1342 313 285 280 267 251 240 236
147 39 23
SacI G_AGCT'C
Cuts at: 0 2584 3423
Size: 2584 839
SanDI GG'GwC_CC
Cuts at: 0 1316 3423
Size: 1316 2107
SbfI CC_TGCA'GG
Cuts at: 0 2052 3423
Size: 2052 1371
ScaI AGT'ACT
Cuts at: 0 2518 3423
Size: 2518 905
SexAI A'CCwGG_T
Cuts at: 0 1064 3423
Size: 1064 2359
SfcI C'TryA_G
Cuts at: 0 658 1501 1848 1875 1882 2048 2432 2769
Size: 658 843 347 27 7 166 384 337
Cuts at: 2769 3423
Size: 654
Fragments arranged by size:
843 658 654 384 347 337 166 27
7
SmaI CCC'GGG
Cuts at: 0 1990 3296 3423
Size: 1990 1306 127
Fragments arranged by size:
1990 1306 127
SmlI C'TyrA_G
Cuts at: 0 548 967 1912 2170 2590 3423
Size: 548 419 945 258 420 833
Fragments arranged by size:
945 833 548 420 419 258
SphI G_CATG'C
Cuts at: 0 2785 3423
Size: 2785 638
StuI AGG'CCT
Cuts at: 0 2404 3423
Size: 2404 1019
StyI C'CwwG_G
Cuts at: 0 35 1224 2162 2342 2441 2717 3423
Size: 35 1189 938 180 99 276 706
Fragments arranged by size:
1189 938 706 276 180 99 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 10 292 387 1550 1701 3163 3385 3423
Size: 10 282 95 1163 151 1462 222 38
Fragments arranged by size:
1462 1163 282 222 151 95 38 10
TatI w'GTAC_w
Cuts at: 0 615 2070 2516 3423
Size: 615 1455 446 907
Fragments arranged by size:
1455 907 615 446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 345 377 1690 1722 3056 3088 3423
Size: 345 32 1313 32 1334 32 335
Fragments arranged by size:
1334 1313 345 335 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1066 3423
Size: 1066 2357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1833 2236 2985 3423
Size: 1833 403 749 438
Fragments arranged by size:
1833 749 438 403
XmaI C'CCGG_G
Cuts at: 0 1988 3294 3423
Size: 1988 1306 129
Fragments arranged by size:
1988 1306 129
XmnI GAAnn'nnTTC
Cuts at: 0 1921 2305 3423
Size: 1921 384 1118
Fragments arranged by size:
1921 1118 384
ZraI GAC'GTC
Cuts at: 0 2633 3423
Size: 2633 790
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AlfI AlwNI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HindIII KpnI MluI
MmeI MscI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SanDI SbfI ScaI SexAI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AleI AloI ApaLI AscI AseI
AsiSI BaeI BbeI BciVI BlpI BmgBI BmrI BmtI
BsiWI BspEI BspHI BssHII BssSI BstBI BstZ17I BtgZI
ClaI DraI DraIII DrdI EagI FseI FspAI HincII
HpaI KasI MfeI MslI NarI NdeI NheI NotI
NruI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI