Restriction map for
pFN21AB4184
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII BcgI BfrI
BsaBI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 908 TCCGGA T^CCGGA
AcsI 139 GAATTC [GA]^AATT[CT]
AcyI 785 GGCGCC G[GA]^CG[CT]C
AhdI 527 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 139 GAATTC [GA]^AATT[CT]
AspEI 527 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 601 CCCGGG C^[CT]CG[GA]G
AvrII 552 CCTAGG C^CTAGG
BbeI 785 GGCGCC GGCGC^C
BbrPI 1458 CACGTG CAC^GTG
BbsI 389 GAAGAC GAAGAC(2/6)
BbvCI 1149 CCTCAGC CCTCAGC(-5/-2)
BciVI 228 GTATCC GTATCC(6/5)
BclI 377 TGATCA T^GATCA
BglI 682 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1406 AGATCT A^GATCT
BlnI 552 CCTAGG C^CTAGG
BlpI 491 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 491 GCTCAGC GC^T[ACGT]AGC
BpuAI 389 GAAGAC GAAGAC(2/6)
BsaAI 1458 CACGTG [CT]AC^GT[GA]
BsaHI 785 GGCGCC G[GA]^CG[CT]C
BsaI 1381 GAGACC GGTCTC(1/5)
BsaWI 908 TCCGGA [AT]^CCGG[AT]
BseAI 908 TCCGGA T^CCGGA
BsoBI 601 CCCGGG C^[CT]CG[GA]G
BspEI 908 TCCGGA T^CCGGA
BsrFI 67 GCCGGC [GA]^CCGG[CT]
BssHII 756 GCGCGC G^CGCGC
CelII 491 GCTCAGC GC^T[ACGT]AGC
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
Eam1105I 527 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 139 GAATTC G^AATTC
FbaI 377 TGATCA T^GATCA
Hin1I 785 GGCGCC G[GA]^CG[CT]C
HincII 1485 GTCAAC GT[CT]^[GA]AC
HindII 1485 GTCAAC GT[CT]^[GA]AC
KasI 785 GGCGCC G^GCGCC
MaeII 1459 ACGT A^CGT
MroI 908 TCCGGA T^CCGGA
MslI 315 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 588 CAGCTG C[AC]G^C[GT]G
NaeI 67 GCCGGC GCC^GGC
NarI 785 GGCGCC GG^CGCC
NgoMIV 67 GCCGGC G^CCGGC
PmaCI 1458 CACGTG CAC^GTG
PmlI 1458 CACGTG CAC^GTG
PpuMI 264 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1090 CTGCAG CTGCA^G
PvuII 588 CAGCTG CAG^CTG
SfcI 1090 CTGCAG C^T[GA][CT]AG
SfoI 785 GGCGCC GGC^GCC
SmaI 601 CCCGGG CCC^GGG
TaqI 1004 TCGA T^CGA
TthHB8I 1004 TCGA T^CGA
XbaI 22 TCTAGA T^CTAGA
XmaCI 601 CCCGGG C^CCGGG
XmaI 601 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AvaII 209 GGTCC G^G[AT]CC
265 GGTCC
BamHI 41 GGATCC G^GATCC
1184 GGATCC
BanI 597 GGTGCC G^G[CT][GA]CC
785 GGCGCC
BssSI 397 CTCGTG CACGAG(-5/-1)
838 CTCGTG
BstAPI 253 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1232 GCATGGTCTGC
MaeIII 829 GTGAC ^GT[ACGT]AC
1177 GTGAC
TfiI 30 GATTC G^A[AT]TC
347 GATTC
Tsp45I 829 GTGAC ^GT[GC]AC
1177 GTGAC
Tsp509I 140 AATT ^AATT
1418 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MboI MboII MflI
MluNI MnlI MscI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TseI TspRI XhoII