(Circular) (Six-base) MAPSORT of: pFN21AB4184.seq Check: 3014 from: 1 to: 6202 pFN21AB4184 6202 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2408 2408 Size: 6202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 3523 3523 Size: 6202 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4265 5649 5917 1632 Size: 2633 1384 268 1917 Fragments arranged by size: 2633 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 2537 4931 2537 Size: 2394 3808 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2066 1184 Size: 882 5320 AlwNI CAG_nnn'CTG Cuts at: 3945 5517 3945 Size: 1572 4630 ApaI G_GGCC'C Cuts at: 2821 3454 3461 2821 Size: 633 7 5562 Fragments arranged by size: 5562 633 7 ApaLI G'TGCA_C Cuts at: 4259 5415 4259 Size: 1156 5046 ApoI r'AATT_y Cuts at: 1493 2144 3511 3737 3889 5998 1493 Size: 651 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 651 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2606 3527 1870 Size: 73 663 921 4545 Fragments arranged by size: 4545 921 663 73 AvrII C'CTAG_G Cuts at: 2557 2557 Size: 6202 BamHI G'GATC_C Cuts at: 2046 3189 3532 3899 2046 Size: 1143 343 367 4349 Fragments arranged by size: 4349 1143 367 343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 2790 3523 4978 618 Size: 325 217 627 815 188 733 1455 1842 Fragments arranged by size: 1842 1455 815 733 627 325 217 188 BanII G_rGCy'C Cuts at: 1391 2475 2498 2556 2720 2821 2871 3104 3454 Size: 1084 23 58 164 101 50 233 350 Cuts at: 3454 3461 3521 1391 Size: 7 60 4072 Fragments arranged by size: 4072 1084 350 233 164 101 60 58 50 23 7 BbeI G_GCGC'C Cuts at: 2794 2794 Size: 6202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2401 928 Size: 971 502 4729 Fragments arranged by size: 4729 971 502 BbvCI CC'TCA_GC Cuts at: 3155 3155 Size: 6202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4393 4427 1903 Size: 34 50 34 2372 34 3678 Fragments arranged by size: 3678 2372 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2244 4094 5304 2244 Size: 1850 1210 3142 Fragments arranged by size: 3142 1850 1210 BclI T'GATC_A Cuts at: 1548 2382 1548 Size: 834 5368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 4813 136 Size: 107 122 71 631 605 1021 2120 1525 Fragments arranged by size: 2120 1525 1021 631 605 122 107 71 BglII A'GATC_T Cuts at: 3411 6197 3411 Size: 2786 3416 BlpI GC'TnA_GC Cuts at: 2497 3619 2497 Size: 1122 5080 Bme1580I G_kGCm'C Cuts at: 1790 2607 2788 2821 3454 3461 4263 5419 1790 Size: 817 181 33 633 7 802 1156 2573 Fragments arranged by size: 2573 1156 817 802 633 181 33 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2973 3933 4891 Size: 416 53 83 399 24 1556 960 958 Cuts at: 4891 442 Size: 1753 Fragments arranged by size: 1753 1556 960 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2196 2600 2762 2771 3497 4862 1239 Size: 957 404 162 9 726 1365 2579 Fragments arranged by size: 2579 1365 957 726 404 162 9 Bpu10I CC'TnA_GC Cuts at: 3155 3155 Size: 6202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4327 5192 5490 4327 Size: 865 298 5039 Fragments arranged by size: 5039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3380 4865 882 Size: 617 1881 1485 2219 Fragments arranged by size: 2219 1881 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 3993 493 Size: 711 2261 528 2702 Fragments arranged by size: 2702 2261 711 528 BsaBI GATnn'nnATC Cuts at: 6196 6196 Size: 6202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2791 4009 4391 5026 Size: 53 83 186 936 1258 1218 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1451 1258 1218 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2913 4635 5033 5307 5454 5865 2913 Size: 1722 398 274 147 411 3250 Fragments arranged by size: 3250 1722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2694 2724 2888 2918 3296 Size: 30 165 30 1291 30 164 30 378 Cuts at: 3296 3326 1178 Size: 30 4054 Fragments arranged by size: 4054 1291 378 165 164 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2493 2651 2759 2768 2771 2839 2893 3484 Size: 1307 158 108 9 3 68 54 591 Cuts at: 3484 1186 Size: 3904 Fragments arranged by size: 3904 1307 591 158 108 68 54 9 3 BseYI C'CCAG_C Cuts at: 2287 2389 3063 5405 5901 2287 Size: 102 674 2342 496 2588 Fragments arranged by size: 2588 2342 674 496 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5629 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3652 4413 5441 1540 Size: 275 186 1651 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2260 2475 2498 2556 2871 3104 4263 4348 5419 Size: 215 23 58 315 233 1159 85 1071 Cuts at: 5419 2260 Size: 3043 Fragments arranged by size: 3043 1159 1071 315 233 215 85 58 23 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2260 2475 2498 2556 2607 2720 2788 Size: 399 470 215 23 58 51 113 68 Cuts at: 2788 2821 2871 3104 3454 3461 3521 4263 4348 Size: 33 50 233 350 7 60 742 85 Cuts at: 4348 5419 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 470 399 350 233 215 113 85 68 60 58 51 50 33 23 7 BspEI T'CCGG_A Cuts at: 2913 5865 2913 Size: 2952 3250 BspHI T'CATG_A Cuts at: 4091 5821 4091 Size: 1730 4472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2258 2408 3068 3557 844 Size: 1414 150 660 489 3489 Fragments arranged by size: 3489 1414 660 489 150 BsrBI CCG'CTC Cuts at: 1394 1823 4089 1394 Size: 429 2266 3507 Fragments arranged by size: 3507 2266 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 4846 5033 1700 Size: 110 262 2774 187 2869 Fragments arranged by size: 2869 2774 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 2761 1925 Size: 836 5366 BssSI C'ACGA_G Cuts at: 2402 2843 4262 5274 2402 Size: 441 1419 1012 3330 Fragments arranged by size: 3330 1419 1012 441 BstAPI GCAn_nnn'nTGC Cuts at: 794 2264 3243 794 Size: 1470 979 3753 Fragments arranged by size: 3753 1470 979 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2449 2599 2287 Size: 162 150 5890 Fragments arranged by size: 5890 162 150 BstYI r'GATC_y Cuts at: 1972 2046 2940 3189 3411 3532 3899 4284 4301 Size: 74 894 249 222 121 367 385 17 Cuts at: 4301 5753 5839 5851 6197 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 894 385 367 346 249 222 121 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2466 2568 2822 2466 Size: 102 254 5846 Fragments arranged by size: 5846 254 102 BtgI C'CryG_G Cuts at: 513 1065 2795 2862 3129 513 Size: 552 1730 67 267 3586 Fragments arranged by size: 3586 1730 552 267 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5463 Fragments arranged by size: 5463 683 56 BtsI GCAGTG_nn' Cuts at: 2183 2258 2863 3718 4512 4532 2183 Size: 75 605 855 794 20 3853 Fragments arranged by size: 3853 855 794 605 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2826 2861 529 Size: 35 2262 35 3870 Fragments arranged by size: 3870 2262 35 35 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2487 2589 Size: 54 1425 50 275 18 447 210 102 Cuts at: 2589 3126 3195 3219 3649 4538 6167 8 Size: 537 69 24 430 889 1629 43 Fragments arranged by size: 1629 1425 889 537 447 430 275 210 102 69 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4132 1353 Size: 2779 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4789 5163 5309 387 Size: 1064 138 3200 374 146 1280 Fragments arranged by size: 3200 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2473 2496 2554 2869 3102 2473 Size: 23 58 315 233 5573 Fragments arranged by size: 5573 315 233 58 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2600 2762 2771 3497 4265 4862 Size: 393 564 404 162 9 726 768 597 Cuts at: 4862 5649 5917 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 768 726 597 564 404 393 268 162 9 EcoO109I rG'GnC_Cy Cuts at: 2270 2818 3457 3458 2270 Size: 548 639 1 5014 Fragments arranged by size: 5014 639 548 1 EcoRI G'AATT_C Cuts at: 2144 3511 3889 2144 Size: 1367 378 4457 Fragments arranged by size: 4457 1367 378 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 2463 2615 2794 3254 5349 1678 Size: 785 152 179 460 2095 2531 Fragments arranged by size: 2531 2095 785 460 179 152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 3004 3036 3296 Size: 32 135 32 951 32 449 32 260 Cuts at: 3296 3328 4843 4875 4917 4949 1373 Size: 32 1515 32 42 32 2626 Fragments arranged by size: 2626 1515 951 449 260 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3546 3792 669 Size: 988 281 1554 54 246 3079 Fragments arranged by size: 3079 1554 988 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KasI G'GCGC_C Cuts at: 2790 2790 Size: 6202 KpnI G_GTAC'C Cuts at: 3527 3527 Size: 6202 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2521 5316 5500 1305 Size: 1216 2795 184 2007 Fragments arranged by size: 2795 2007 1216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 2489 2591 3128 3197 Size: 54 1425 343 447 210 102 537 69 Cuts at: 3197 3221 10 Size: 24 2991 Fragments arranged by size: 2991 1425 537 447 343 210 102 69 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2324 4160 4519 4678 518 Size: 648 1158 1836 359 159 2042 Fragments arranged by size: 2042 1836 1158 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2595 3618 4295 5443 5688 1606 Size: 989 1023 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 989 677 245 NaeI GCC'GGC Cuts at: 2074 2074 Size: 6202 NarI GG'CG_CC Cuts at: 2791 2791 Size: 6202 NcoI C'CATG_G Cuts at: 513 1065 2795 2862 3129 513 Size: 552 1730 67 267 3586 Fragments arranged by size: 3586 1730 552 267 67 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2072 2072 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 3560 5105 1132 Size: 97 2331 1545 2229 Fragments arranged by size: 2331 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2239 3454 1348 Size: 891 1215 4096 Fragments arranged by size: 4096 1215 891 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6202 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PmlI CAC'GTG Cuts at: 3465 3465 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 2270 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6202 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PspOMI G'GGCC_C Cuts at: 2817 3450 3457 2817 Size: 633 7 5562 Fragments arranged by size: 5562 633 7 PstI C_TGCA'G Cuts at: 830 3099 3554 830 Size: 2269 455 3478 Fragments arranged by size: 3478 2269 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6202 PvuII CAG'CTG Cuts at: 2595 2595 Size: 6202 SacI G_AGCT'C Cuts at: 2475 2498 2556 2871 3104 2475 Size: 23 58 315 233 5573 Fragments arranged by size: 5573 315 233 58 23 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 4450 6162 4450 Size: 1712 4490 SfcI C'TryA_G Cuts at: 826 1045 3095 3550 4685 5366 5557 826 Size: 219 2050 455 1135 681 191 1471 Fragments arranged by size: 2050 1471 1135 681 455 219 191 SfoI GGC'GCC Cuts at: 2792 2792 Size: 6202 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 2608 3529 1872 Size: 736 921 4545 Fragments arranged by size: 4545 921 736 SmlI C'TyrA_G Cuts at: 820 1017 1943 4306 5207 5469 820 Size: 197 926 2363 901 262 1553 Fragments arranged by size: 2363 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 2247 2573 2687 3226 3308 1517 Size: 730 326 114 539 82 4411 Fragments arranged by size: 4411 730 539 326 114 82 StyI C'CwwG_G Cuts at: 513 1065 2274 2557 2795 2862 3129 3337 3417 Size: 552 1209 283 238 67 267 208 80 Cuts at: 3417 3641 513 Size: 224 3074 Fragments arranged by size: 3074 1209 552 283 267 238 224 208 80 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4229 4246 4399 4584 5029 325 Size: 1255 2649 17 153 185 445 1498 Fragments arranged by size: 2649 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4448 6160 96 Size: 274 80 33 51 1443 2471 1712 138 Fragments arranged by size: 2471 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6162 6194 6162 Size: 32 6170 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6202 XbaI T'CTAG_A Cuts at: 2027 3538 2027 Size: 1511 4691 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 2606 3527 1870 Size: 736 921 4545 Fragments arranged by size: 4545 921 736 XmnI GAAnn'nnTTC Cuts at: 4331 4331 Size: 6202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI