Restriction map for
pFN21AB4184
(Linear) (Six-base) MAPSORT of: KIBB4184.seq Check: 3794 from: 1 to: 1497
KIBB4184.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 403 1497
Size: 403 1094
AhdI GACnn_n'nnGTC
Cuts at: 0 532 1497
Size: 532 965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 1497
Size: 61 1436
ApaI G_GGCC'C
Cuts at: 0 816 1449 1456 1497
Size: 816 633 7 41
Fragments arranged by size:
816 633 41 7
ApoI r'AATT_y
Cuts at: 0 139 1497
Size: 139 1358
AvaI C'yCGr_G
Cuts at: 0 601 1497
Size: 601 896
AvrII C'CTAG_G
Cuts at: 0 552 1497
Size: 552 945
BamHI G'GATC_C
Cuts at: 0 41 1184 1497
Size: 41 1143 313
Fragments arranged by size:
1143 313 41
BanI G'GyrC_C
Cuts at: 0 597 785 1497
Size: 597 188 712
Fragments arranged by size:
712 597 188
BanII G_rGCy'C
Cuts at: 0 470 493 551 715 816 866 1099 1449
Size: 470 23 58 164 101 50 233 350
Cuts at: 1449 1456 1497
Size: 7 41
Fragments arranged by size:
470 350 233 164 101 58 50 41
23 7
BbeI G_GCGC'C
Cuts at: 0 789 1497
Size: 789 708
BbsI GAAGACnn'nnnn_
Cuts at: 0 396 1497
Size: 396 1101
BbvCI CC'TCA_GC
Cuts at: 0 1150 1497
Size: 1150 347
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 1497
Size: 239 1258
BclI T'GATC_A
Cuts at: 0 377 1497
Size: 377 1120
BglI GCCn_nnn'nGGC
Cuts at: 0 688 1497
Size: 688 809
BglII A'GATC_T
Cuts at: 0 1406 1497
Size: 1406 91
BlpI GC'TnA_GC
Cuts at: 0 492 1497
Size: 492 1005
Bme1580I G_kGCm'C
Cuts at: 0 602 783 816 1449 1456 1497
Size: 602 181 33 633 7 41
Fragments arranged by size:
633 602 181 41 33 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 968 1497
Size: 968 529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 595 757 766 1492 1497
Size: 191 404 162 9 726 5
Fragments arranged by size:
726 404 191 162 9 5
Bpu10I CC'TnA_GC
Cuts at: 0 1150 1497
Size: 1150 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 1375 1497
Size: 1375 122
BsaAI yAC'GTr
Cuts at: 0 1460 1497
Size: 1460 37
BsaHI Gr'CG_yC
Cuts at: 0 786 1497
Size: 786 711
BsaWI w'CCGG_w
Cuts at: 0 908 1497
Size: 908 589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 689 719 883 913 1291 1321 1497
Size: 689 30 164 30 378 30 176
Fragments arranged by size:
689 378 176 164 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 488 646 754 763 766 834 888 1479
Size: 488 158 108 9 3 68 54 591
Cuts at: 1479 1497
Size: 18
Fragments arranged by size:
591 488 158 108 68 54 18 9
3
BseYI C'CCAG_C
Cuts at: 0 282 384 1058 1497
Size: 282 102 674 439
Fragments arranged by size:
674 439 282 102
BsiHKAI G_wGCw'C
Cuts at: 0 255 470 493 551 866 1099 1497
Size: 255 215 23 58 315 233 398
Fragments arranged by size:
398 315 255 233 215 58 23
Bsp1286I G_dGCh'C
Cuts at: 0 255 470 493 551 602 715 783 816
Size: 255 215 23 58 51 113 68 33
Cuts at: 816 866 1099 1449 1456 1497
Size: 50 233 350 7 41
Fragments arranged by size:
350 255 233 215 113 68 58 51
50 41 33 23 7
BspEI T'CCGG_A
Cuts at: 0 908 1497
Size: 908 589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 253 403 1063 1497
Size: 253 150 660 434
Fragments arranged by size:
660 434 253 150
BsrFI r'CCGG_y
Cuts at: 0 67 1497
Size: 67 1430
BssHII G'CGCG_C
Cuts at: 0 756 1497
Size: 756 741
BssSI C'ACGA_G
Cuts at: 0 397 838 1497
Size: 397 441 659
Fragments arranged by size:
659 441 397
BstAPI GCAn_nnn'nTGC
Cuts at: 0 259 1238 1497
Size: 259 979 259
Fragments arranged by size:
979 259 259
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 444 594 1497
Size: 282 162 150 903
Fragments arranged by size:
903 282 162 150
BstYI r'GATC_y
Cuts at: 0 41 935 1184 1406 1497
Size: 41 894 249 222 91
Fragments arranged by size:
894 249 222 91 41
Bsu36I CC'TnA_GG
Cuts at: 0 461 563 817 1497
Size: 461 102 254 680
Fragments arranged by size:
680 461 254 102
BtgI C'CryG_G
Cuts at: 0 790 857 1124 1497
Size: 790 67 267 373
Fragments arranged by size:
790 373 267 67
BtsI GCAGTG_nn'
Cuts at: 0 178 253 858 1497
Size: 178 75 605 639
Fragments arranged by size:
639 605 178 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 821 856 1497
Size: 821 35 641
Fragments arranged by size:
821 641 35
EaeI y'GGCC_r
Cuts at: 0 272 482 584 1121 1190 1214 1497
Size: 272 210 102 537 69 24 283
Fragments arranged by size:
537 283 272 210 102 69 24
EcoICRI GAG'CTC
Cuts at: 0 468 491 549 864 1097 1497
Size: 468 23 58 315 233 400
Fragments arranged by size:
468 400 315 233 58 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 595 757 766 1492 1497
Size: 191 404 162 9 726 5
Fragments arranged by size:
726 404 191 162 9 5
EcoO109I rG'GnC_Cy
Cuts at: 0 265 813 1452 1453 1497
Size: 265 548 639 1 44
Fragments arranged by size:
639 548 265 44 1
EcoRI G'AATT_C
Cuts at: 0 139 1497
Size: 139 1358
HaeII r_GCGC'y
Cuts at: 0 458 610 789 1249 1497
Size: 458 152 179 460 248
Fragments arranged by size:
460 458 248 179 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 999 1031 1291 1323 1497
Size: 518 32 449 32 260 32 174
Fragments arranged by size:
518 449 260 174 32 32 32
HincII GTy'rAC
Cuts at: 0 1487 1497
Size: 1487 10
KasI G'GCGC_C
Cuts at: 0 785 1497
Size: 785 712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1497
Size: 516 981
MscI TGG'CCA
Cuts at: 0 274 484 586 1123 1192 1216 1497
Size: 274 210 102 537 69 24 281
Fragments arranged by size:
537 281 274 210 102 69 24
MslI CAynn'nnrTG
Cuts at: 0 319 1497
Size: 319 1178
MspA1I CmG'CkG
Cuts at: 0 590 1497
Size: 590 907
NaeI GCC'GGC
Cuts at: 0 69 1497
Size: 69 1428
NarI GG'CG_CC
Cuts at: 0 786 1497
Size: 786 711
NcoI C'CATG_G
Cuts at: 0 790 857 1124 1497
Size: 790 67 267 373
Fragments arranged by size:
790 373 267 67
NgoMIV G'CCGG_C
Cuts at: 0 67 1497
Size: 67 1430
PasI CC'CwG_GG
Cuts at: 0 234 1449 1497
Size: 234 1215 48
Fragments arranged by size:
1215 234 48
PmlI CAC'GTG
Cuts at: 0 1460 1497
Size: 1460 37
PpuMI rG'GwC_Cy
Cuts at: 0 265 1497
Size: 265 1232
PspOMI G'GGCC_C
Cuts at: 0 812 1445 1452 1497
Size: 812 633 7 45
Fragments arranged by size:
812 633 45 7
PstI C_TGCA'G
Cuts at: 0 1094 1497
Size: 1094 403
PvuII CAG'CTG
Cuts at: 0 590 1497
Size: 590 907
SacI G_AGCT'C
Cuts at: 0 470 493 551 866 1099 1497
Size: 470 23 58 315 233 398
Fragments arranged by size:
470 398 315 233 58 23
SfcI C'TryA_G
Cuts at: 0 1090 1497
Size: 1090 407
SfoI GGC'GCC
Cuts at: 0 787 1497
Size: 787 710
SmaI CCC'GGG
Cuts at: 0 603 1497
Size: 603 894
StuI AGG'CCT
Cuts at: 0 242 568 682 1221 1303 1497
Size: 242 326 114 539 82 194
Fragments arranged by size:
539 326 242 194 114 82
StyI C'CwwG_G
Cuts at: 0 269 552 790 857 1124 1332 1412 1497
Size: 269 283 238 67 267 208 80 85
Fragments arranged by size:
283 269 267 238 208 85 80 67
XbaI T'CTAG_A
Cuts at: 0 22 1497
Size: 22 1475
XmaI C'CCGG_G
Cuts at: 0 601 1497
Size: 601 896
Enzymes that do cut:
AarI AhdI AlfI ApaI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PmlI PpuMI PspOMI
PstI PvuII SacI SfcI SfoI SmaI StuI StyI
XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaLI AscI AseI AsiSI
BaeI BcgI BfrBI BmgBI BmtI BplI BpuEI BsaBI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BsrGI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EarI EciI EcoNI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XcmI XhoI
XmnI ZraI