Restriction map for
pFN21AB4182
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# NO CUT ENZYMES ( 122 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AlwNI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BfaI BfrI BlnI BpuAI BsaBI
BsaI BsaWI BseAI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoNI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 658 GACGTC GACGT^C
AhdI 181 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 34 GTGCAC G^TGCAC
AlwI 555 GGATC GGATC(4/5)
AocI 941 CCTCAGG CC^T[ACGT]AGG
ApaLI 34 GTGCAC G^TGCAC
AspEI 181 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 34 GTGCAC G[AT]GC[AT]^C
BbeI 730 GGCGCC GGCGC^C
BbvCI 663 CCTCAGC CCTCAGC(-5/-2)
BclI 77 TGATCA T^GATCA
BglI 766 GCCGTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 564 AGATCT A^GATCT
BlpI 881 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 881 GCTCAGC GC^T[ACGT]AGC
BsaAI 588 CACGTA [CT]AC^GT[GA]
BsgI 936 CTGCAC GTGCAG(16/14)
BsiEI 68 CGGCCG CG[GA][CT]^CG
BsiHKAI 34 GTGCAC G[AT]GC[AT]^C
BsmBI 131 GAGACG CGTCTC(1/5)
BsmI 342 GAATGC GAATGC(1/-1)
Bsu36I 941 CCTCAGG CC^T[ACGT]AGG
CelII 881 GCTCAGC GC^T[ACGT]AGC
EagI 68 CGGCCG C^GGCCG
Eam1105I 181 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 207 CTCTTC CTCTTC(1/4)
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
Eco81I 941 CCTCAGG CC^T[ACGT]AGG
FbaI 77 TGATCA T^GATCA
HgaI 133 GACGC GACGC(5/10)
KasI 730 GGCGCC G^GCGCC
Ksp632I 207 CTCTTC CTCTTC(1/4)
MslI 718 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 730 GGCGCC GG^CGCC
NcoI 717 CCATGG C^CATGG
PflMI 834 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 689 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 933 CAGCTG CAG^CTG
SfoI 730 GGCGCC GGC^GCC
SmaI 992 CCCGGG CCC^GGG
SphI 603 GCATGC GCATG^C
StuI 545 AGGCCT AGG^CCT
TspRI 485 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 834 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 992 CCCGGG C^CCGGG
XmaI 992 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 381 CGCG CG^CG
507 CGCG
AfaI 591 GTAC GT^AC
762 GTAC
ApaI 640 GGGCCC GGGCC^C
989 GGGCCC
BsiWI 590 CGTACG C^GTACG
761 CGTACG
BsrBI 56 GAGCGG CCGCTC(-3/-3)
286 GAGCGG
BsrI 18 ACTGG ACTGG(1/-1)
395 CCAGT
BstEII 273 GGTGACC G^GT[ACGT]ACC
712 GGTTACC
BstPI 273 GGTGACC G^GT[ACGT]ACC
712 GGTTACC
BstUI 381 CGCG CG^CG
507 CGCG
BstYI 555 GGATCT [GA]^GATC[CT]
564 AGATCT
EaeI 68 CGGCCG [CT]^GGCC[GA]
120 TGGCCG
EcoO65I 273 GGTGACC G^GT[ACGT]ACC
712 GGTTACC
KspI 380 CCGCGG CCGC^GG
506 CCGCGG
MflI 555 GGATCT [GA]^GATC[CT]
564 AGATCT
MvnI 381 CGCG CG^CG
507 CGCG
NspI 302 ACATGC [GA]CATG^[CT]
603 GCATGC
RsaI 591 GTAC GT^AC
762 GTAC
SacII 380 CCGCGG CCGC^GG
506 CCGCGG
SfaNI 668 GCATC GCATC(5/9)
947 GCATC
SmlI 10 CTCAAG C^T[CT][GA]AG
1005 CTCAAG
TaqI 536 TCGA T^CGA
632 TCGA
TthHB8I 536 TCGA T^CGA
632 TCGA
XhoII 555 GGATCT [GA]^GATC[CT]
564 AGATCT
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcyI AluI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI StyI TseI
Tsp45I