(Circular) (Six-base) MAPSORT of: pFN21AB4182.seq Check: 9342 from: 1 to: 5773 pFN21AB4182 5773 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2667 3583 278 Size: 53 83 186 936 1131 916 2468 Fragments arranged by size: 2468 1131 936 916 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 3836 5220 5488 1632 Size: 992 1212 1384 268 1917 Fragments arranged by size: 1917 1384 1212 992 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 2191 4502 2191 Size: 2311 3462 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaI G_GGCC'C Cuts at: 2649 2998 2649 Size: 349 5424 ApaLI G'TGCA_C Cuts at: 2039 3830 4986 2039 Size: 1791 1156 2826 Fragments arranged by size: 2826 1791 1156 ApoI r'AATT_y Cuts at: 1493 3082 3308 3460 5569 1493 Size: 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2699 2997 3098 1870 Size: 73 115 641 298 101 4545 Fragments arranged by size: 4545 641 298 115 101 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2735 2744 3094 4549 Size: 325 217 627 609 339 9 350 1455 Cuts at: 4549 618 Size: 1842 Fragments arranged by size: 1842 1455 627 609 350 339 325 217 9 BanII G_rGCy'C Cuts at: 1391 2184 2318 2649 2998 3092 1391 Size: 793 134 331 349 94 4072 Fragments arranged by size: 4072 793 349 331 134 94 BbeI G_GCGC'C Cuts at: 2739 2739 Size: 5773 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4802 BbvCI CC'TCA_GC Cuts at: 2669 2669 Size: 5773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3964 3998 1903 Size: 34 50 34 1943 34 3678 Fragments arranged by size: 3678 1943 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 2082 1548 Size: 534 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2777 4384 136 Size: 107 122 71 631 605 1105 1607 1525 Fragments arranged by size: 1607 1525 1105 631 605 122 107 71 BglII A'GATC_T Cuts at: 2569 5768 2569 Size: 3199 2574 BlpI GC'TnA_GC Cuts at: 2887 3190 2887 Size: 303 5470 Bme1580I G_kGCm'C Cuts at: 1790 2043 2399 2480 2496 2586 2649 2998 3834 Size: 253 356 81 16 90 63 349 836 Cuts at: 3834 4990 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 836 356 349 253 90 81 63 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2084 2084 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2867 2920 2978 4433 1239 Size: 1628 53 58 1455 2579 Fragments arranged by size: 2579 1628 1455 58 53 Bpu10I CC'TnA_GC Cuts at: 2669 2669 Size: 5773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 2995 3898 4763 5061 2000 Size: 995 903 865 298 2712 Fragments arranged by size: 2712 995 903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2595 3564 493 Size: 711 1391 969 2702 Fragments arranged by size: 2702 1391 969 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2664 2736 3580 Size: 53 83 186 936 606 525 72 844 Cuts at: 3580 3962 4597 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 844 635 606 525 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 2743 2773 2810 Size: 30 165 30 817 30 493 30 37 Cuts at: 2810 2840 2912 2942 1178 Size: 30 72 30 4009 Fragments arranged by size: 4009 817 493 165 72 37 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2498 2528 2702 2717 2975 3062 3065 Size: 1297 15 30 174 15 258 87 3 Cuts at: 3065 3068 1186 Size: 3 3891 Fragments arranged by size: 3891 1297 258 174 87 30 15 15 3 3 BseYI C'CCAG_C Cuts at: 2095 2311 2364 2656 2876 4976 5472 2095 Size: 216 53 292 220 2100 496 2396 Fragments arranged by size: 2396 2100 496 292 220 216 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2926 1163 Size: 573 1190 4010 Fragments arranged by size: 4010 1190 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 3223 3984 5012 1540 Size: 275 186 75 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 75 BsiHKAI G_wGCw'C Cuts at: 2043 3834 3919 4990 2043 Size: 1791 85 1071 2826 Fragments arranged by size: 2826 1791 1071 85 BsiWI C'GTAC_G Cuts at: 2595 2766 2595 Size: 171 5602 BsmI GAATG_Cn' Cuts at: 2353 3284 3377 2353 Size: 931 93 4749 Fragments arranged by size: 4749 931 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2130 2130 Size: 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2184 2318 2399 2480 2496 2586 Size: 399 253 141 134 81 81 16 90 Cuts at: 2586 2649 2998 3092 3834 3919 4990 1391 Size: 63 349 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 253 141 134 94 90 85 81 81 63 16 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3128 844 Size: 2284 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2063 2293 3660 1394 Size: 429 240 230 1367 3507 Fragments arranged by size: 3507 1367 429 240 230 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4417 4604 1700 Size: 110 2607 187 2869 Fragments arranged by size: 2869 2607 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 2278 2717 1184 Size: 1094 439 4240 Fragments arranged by size: 4240 1094 439 BstYI r'GATC_y Cuts at: 1972 2560 2569 3103 3470 3855 3872 5324 5410 Size: 588 9 534 367 385 17 1452 86 Cuts at: 5410 5422 5768 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 588 534 385 367 346 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2947 2947 Size: 5773 BtgI C'CryG_G Cuts at: 513 1065 2385 2511 2722 2772 513 Size: 552 1320 126 211 50 3514 Fragments arranged by size: 3514 1320 552 211 126 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2478 2742 487 Size: 683 56 1252 264 3518 Fragments arranged by size: 3518 1252 683 264 56 BtsI GCAGTG_nn' Cuts at: 2496 3289 4083 4103 2496 Size: 793 794 20 4166 Fragments arranged by size: 4166 794 793 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2125 3220 Size: 54 1425 50 275 18 243 52 1095 Cuts at: 3220 4109 5738 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1095 889 275 243 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2073 3220 1537 Size: 275 261 1147 4090 Fragments arranged by size: 4090 1147 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 2218 3703 1353 Size: 865 1485 3423 Fragments arranged by size: 3423 1485 865 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 4360 4734 4880 387 Size: 1064 138 507 2264 374 146 1280 Fragments arranged by size: 2264 1280 1064 507 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 2867 2920 2978 3836 4433 5220 Size: 393 992 243 53 58 858 597 787 Cuts at: 5220 5488 1239 Size: 268 1524 Fragments arranged by size: 1524 992 858 787 597 393 268 243 58 53 EcoO109I rG'GnC_Cy Cuts at: 2645 2646 2695 2994 2645 Size: 1 49 299 5424 Fragments arranged by size: 5424 299 49 1 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 2053 2091 2739 4920 1678 Size: 375 38 648 2181 2531 Fragments arranged by size: 2531 2181 648 375 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2810 2842 2912 Size: 32 135 32 605 32 601 32 70 Cuts at: 2912 2944 4414 4446 4488 4520 1373 Size: 32 1470 32 42 32 2626 Fragments arranged by size: 2626 1470 605 601 135 70 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KasI G'GCGC_C Cuts at: 2735 2735 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 4887 5071 1305 Size: 1175 2407 184 2007 Fragments arranged by size: 2407 2007 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3951 Fragments arranged by size: 3951 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2727 3731 4090 4249 518 Size: 648 1561 1004 359 159 2042 Fragments arranged by size: 2042 1561 1004 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2387 2513 2741 2870 2940 3189 3866 Size: 489 292 126 228 129 70 249 677 Cuts at: 3866 5014 5259 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 292 249 245 228 129 126 70 NarI GG'CG_CC Cuts at: 2736 2736 Size: 5773 NcoI C'CATG_G Cuts at: 513 1065 2722 513 Size: 552 1657 3564 Fragments arranged by size: 3564 1657 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 2311 2612 3131 4676 1132 Size: 97 1082 301 519 1545 2229 Fragments arranged by size: 2229 1545 1082 519 301 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 4036 PfoI T'CCnGG_A Cuts at: 2243 2955 2243 Size: 712 5061 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2695 Size: 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2645 2994 2645 Size: 349 5424 PstI C_TGCA'G Cuts at: 830 3125 830 Size: 2295 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2940 2940 Size: 5773 SacII CC_GC'GG Cuts at: 2388 2514 2388 Size: 126 5647 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SanDI GG'GwC_CC Cuts at: 2695 2695 Size: 5773 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 3121 4256 4937 5128 826 Size: 219 2076 1135 681 191 1471 Fragments arranged by size: 2076 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2737 2737 Size: 5773 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 2999 3100 1872 Size: 1127 101 4545 Fragments arranged by size: 4545 1127 101 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3010 3877 4778 5040 820 Size: 197 926 72 995 867 901 262 1553 Fragments arranged by size: 1553 995 926 901 867 262 197 72 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 2612 3131 1132 Size: 1480 519 3774 Fragments arranged by size: 3774 1480 519 SrfI GCCC'GGGC Cuts at: 2999 2999 Size: 5773 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 2552 1517 Size: 1035 4738 StyI C'CwwG_G Cuts at: 513 1065 2196 2586 2722 3212 513 Size: 552 1131 390 136 490 3074 Fragments arranged by size: 3074 1131 552 490 390 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2648 2768 3800 3817 3970 4155 4600 Size: 1255 1068 120 1032 17 153 185 445 Cuts at: 4600 325 Size: 1498 Fragments arranged by size: 1498 1255 1068 1032 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4019 5731 96 Size: 274 80 33 51 1443 2042 1712 138 Fragments arranged by size: 2042 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2743 2775 2843 2875 5733 5765 2743 Size: 32 68 32 2858 32 2751 Fragments arranged by size: 2858 2751 68 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 2997 3098 1870 Size: 1127 101 4545 Fragments arranged by size: 4545 1127 101 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2665 3581 276 Size: 53 83 186 936 1131 916 2468 Fragments arranged by size: 2468 1131 936 916 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SwaI XcmI