Restriction map for
pFN21AB4182
(Linear) (Six-base) MAPSORT of: KIBB4182.seq Check: 3808 from: 1 to: 1068
KIBB4182.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AatII G_ACGT'C
Cuts at: 0 662 1068
Size: 662 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1068
Size: 619 449
AhdI GACnn_n'nnGTC
Cuts at: 0 186 1068
Size: 186 882
ApaI G_GGCC'C
Cuts at: 0 644 993 1068
Size: 644 349 75
Fragments arranged by size:
644 349 75
ApaLI G'TGCA_C
Cuts at: 0 34 1068
Size: 34 1034
AvaI C'yCGr_G
Cuts at: 0 53 694 992 1068
Size: 53 641 298 76
Fragments arranged by size:
641 298 76 53
BanI G'GyrC_C
Cuts at: 0 391 730 739 1068
Size: 391 339 9 329
Fragments arranged by size:
391 339 329 9
BanII G_rGCy'C
Cuts at: 0 179 313 644 993 1068
Size: 179 134 331 349 75
Fragments arranged by size:
349 331 179 134 75
BbeI G_GCGC'C
Cuts at: 0 734 1068
Size: 734 334
BbvCI CC'TCA_GC
Cuts at: 0 664 1068
Size: 664 404
BclI T'GATC_A
Cuts at: 0 77 1068
Size: 77 991
BglI GCCn_nnn'nGGC
Cuts at: 0 772 1068
Size: 772 296
BglII A'GATC_T
Cuts at: 0 564 1068
Size: 564 504
BlpI GC'TnA_GC
Cuts at: 0 882 1068
Size: 882 186
Bme1580I G_kGCm'C
Cuts at: 0 38 394 475 491 581 644 993 1068
Size: 38 356 81 16 90 63 349 75
Fragments arranged by size:
356 349 90 81 75 63 38 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 1068
Size: 79 989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 915 973 1068
Size: 862 53 58 95
Fragments arranged by size:
862 95 58 53
Bpu10I CC'TnA_GC
Cuts at: 0 664 1068
Size: 664 404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1068
Size: 990 78
BsaAI yAC'GTr
Cuts at: 0 590 1068
Size: 590 478
BsaHI Gr'CG_yC
Cuts at: 0 134 659 731 1068
Size: 134 525 72 337
Fragments arranged by size:
525 337 134 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 738 768 805 835 907 937
Size: 215 30 493 30 37 30 72 30
Cuts at: 937 1068
Size: 131
Fragments arranged by size:
493 215 131 72 37 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 493 523 697 712 970 1057 1060
Size: 478 15 30 174 15 258 87 3
Cuts at: 1060 1063 1068
Size: 3 5
Fragments arranged by size:
478 258 174 87 30 15 15 5
3 3
BseYI C'CCAG_C
Cuts at: 0 90 306 359 651 871 1068
Size: 90 216 53 292 220 197
Fragments arranged by size:
292 220 216 197 90 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1068
Size: 921 147
BsiEI CG_ry'CG
Cuts at: 0 71 1068
Size: 71 997
BsiHKAI G_wGCw'C
Cuts at: 0 38 1068
Size: 38 1030
BsiWI C'GTAC_G
Cuts at: 0 590 761 1068
Size: 590 171 307
Fragments arranged by size:
590 307 171
BsmI GAATG_Cn'
Cuts at: 0 348 1068
Size: 348 720
BsmBI CGTCTCn'nnnn_
Cuts at: 0 125 1068
Size: 125 943
Bsp1286I G_dGCh'C
Cuts at: 0 38 179 313 394 475 491 581 644
Size: 38 141 134 81 81 16 90 63
Cuts at: 644 993 1068
Size: 349 75
Fragments arranged by size:
349 141 134 90 81 81 75 63
38 16
BsrBI CCG'CTC
Cuts at: 0 58 288 1068
Size: 58 230 780
Fragments arranged by size:
780 230 58
BstEII G'GTnAC_C
Cuts at: 0 273 712 1068
Size: 273 439 356
Fragments arranged by size:
439 356 273
BstYI r'GATC_y
Cuts at: 0 555 564 1068
Size: 555 9 504
Fragments arranged by size:
555 504 9
Bsu36I CC'TnA_GG
Cuts at: 0 942 1068
Size: 942 126
BtgI C'CryG_G
Cuts at: 0 380 506 717 767 1068
Size: 380 126 211 50 301
Fragments arranged by size:
380 301 211 126 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 473 737 1068
Size: 473 264 331
Fragments arranged by size:
473 331 264
BtsI GCAGTG_nn'
Cuts at: 0 491 1068
Size: 491 577
EaeI y'GGCC_r
Cuts at: 0 68 120 1068
Size: 68 52 948
Fragments arranged by size:
948 68 52
EagI C'GGCC_G
Cuts at: 0 68 1068
Size: 68 1000
EarI CTCTTCn'nnn_
Cuts at: 0 213 1068
Size: 213 855
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 1068
Size: 91 977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 862 915 973 1068
Size: 619 243 53 58 95
Fragments arranged by size:
619 243 95 58 53
EcoO109I rG'GnC_Cy
Cuts at: 0 640 641 690 989 1068
Size: 640 1 49 299 79
Fragments arranged by size:
640 299 79 49 1
HaeII r_GCGC'y
Cuts at: 0 48 86 734 1068
Size: 48 38 648 334
Fragments arranged by size:
648 334 48 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 805 837 907 939 1068
Size: 172 32 601 32 70 32 129
Fragments arranged by size:
601 172 129 70 32 32 32
KasI G'GCGC_C
Cuts at: 0 730 1068
Size: 730 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1068
Size: 475 593
MslI CAynn'nnrTG
Cuts at: 0 722 1068
Size: 722 346
MspA1I CmG'CkG
Cuts at: 0 90 382 508 736 865 935 1068
Size: 90 292 126 228 129 70 133
Fragments arranged by size:
292 228 133 129 126 90 70
NarI GG'CG_CC
Cuts at: 0 731 1068
Size: 731 337
NcoI C'CATG_G
Cuts at: 0 717 1068
Size: 717 351
NspI r_CATG'y
Cuts at: 0 306 607 1068
Size: 306 301 461
Fragments arranged by size:
461 306 301
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 1068
Size: 840 228
PfoI T'CCnGG_A
Cuts at: 0 238 950 1068
Size: 238 712 118
Fragments arranged by size:
712 238 118
PpuMI rG'GwC_Cy
Cuts at: 0 690 1068
Size: 690 378
PspOMI G'GGCC_C
Cuts at: 0 640 989 1068
Size: 640 349 79
Fragments arranged by size:
640 349 79
PvuII CAG'CTG
Cuts at: 0 935 1068
Size: 935 133
SacII CC_GC'GG
Cuts at: 0 383 509 1068
Size: 383 126 559
Fragments arranged by size:
559 383 126
SanDI GG'GwC_CC
Cuts at: 0 690 1068
Size: 690 378
SfoI GGC'GCC
Cuts at: 0 732 1068
Size: 732 336
SmaI CCC'GGG
Cuts at: 0 994 1068
Size: 994 74
SmlI C'TyrA_G
Cuts at: 0 10 1005 1068
Size: 10 995 63
Fragments arranged by size:
995 63 10
SphI G_CATG'C
Cuts at: 0 607 1068
Size: 607 461
SrfI GCCC'GGGC
Cuts at: 0 994 1068
Size: 994 74
StuI AGG'CCT
Cuts at: 0 547 1068
Size: 547 521
StyI C'CwwG_G
Cuts at: 0 191 581 717 1068
Size: 191 390 136 351
Fragments arranged by size:
390 351 191 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 763 1068
Size: 643 120 305
Fragments arranged by size:
643 305 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 738 770 838 870 1068
Size: 738 32 68 32 198
Fragments arranged by size:
738 198 68 32 32
XmaI C'CCGG_G
Cuts at: 0 992 1068
Size: 992 76
ZraI GAC'GTC
Cuts at: 0 660 1068
Size: 660 408
Enzymes that do cut:
AatII AcuI AhdI ApaI ApaLI AvaI BanI BanII
BbeI BbvCI BclI BglI BglII BlpI Bme1580I BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BsrBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI
MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI
PspOMI PvuII SacII SanDI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BmgBI
BmrI BmtI BsaI BsaBI BsaWI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI