Restriction map for
pFN21AB4062
(Circular) (Six-base) MAPSORT of: pFN21AB4062.seq Check: 4961 from: 1 to: 5230
pFN21AB4062 5230 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4677 4945 1632
Size: 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 2250 3959 2250
Size: 1709 3521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2311 2380 2448 2973 4545 2311
Size: 69 68 525 1572 2996
Fragments arranged by size:
2996 1572 525 69 68
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2049 2560 2927 2049
Size: 511 367 4352
Fragments arranged by size:
4352 511 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2502 2549 1391
Size: 1111 47 4072
Fragments arranged by size:
4072 1111 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2224 3841 136
Size: 107 122 71 631 605 552 1617 1525
Fragments arranged by size:
1617 1525 631 605 552 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2250 2429 2961
Size: 416 53 83 399 24 833 179 532
Cuts at: 2961 3919 442
Size: 958 1753
Fragments arranged by size:
1753 958 833 532 416 399 179 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3890 1239
Size: 2651 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 3893 882
Size: 617 759 1635 2219
Fragments arranged by size:
2219 1635 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 3037 3419 4054
Size: 53 83 186 936 813 691 382 635
Cuts at: 4054 275
Size: 1451
Fragments arranged by size:
1451 936 813 691 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 2107 4433 4929 2107
Size: 2326 496 2408
Fragments arranged by size:
2408 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2477 1163
Size: 573 561 180 3916
Fragments arranged by size:
3916 573 561 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 2219 2281 2287 2680 3441
Size: 275 186 75 143 62 6 393 761
Cuts at: 3441 4469 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 393 275 186 143 75
62 6
BsiHKAI G_wGCw'C
Cuts at: 3291 3376 4447 3291
Size: 85 1071 4074
Fragments arranged by size:
4074 1071 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 2147
Size: 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 2549 3291 3376 4447 1391
Size: 399 712 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 399 85 47
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2585 844
Size: 1544 197 3489
Fragments arranged by size:
3489 1544 197
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3117 1394
Size: 429 398 896 3507
Fragments arranged by size:
3507 896 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 3874 4061 1700
Size: 110 265 1799 187 2869
Fragments arranged by size:
2869 1799 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 2094 96
Size: 1998 3232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 2402 3290 4302 2402
Size: 888 1012 3330
Fragments arranged by size:
3330 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2380 794
Size: 1586 3644
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 2119 1184
Size: 935 4295
BstYI r'GATC_y
Cuts at: 1972 2049 2211 2406 2560 2927 3312 3329 4781
Size: 77 162 195 154 367 385 17 1452
Cuts at: 4781 4867 4879 5225 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 195 162 154
86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 2476 2476
Size: 5230
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4491
Fragments arranged by size:
4491 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 2292 1554
Size: 738 4492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2200 2216
Size: 54 1425 50 275 18 243 127 16
Cuts at: 2216 2677 3566 5195 8
Size: 461 889 1629 43
Fragments arranged by size:
1629 1425 889 461 275 243 127 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2216 2677 1537
Size: 275 261 143 461 4090
Fragments arranged by size:
4090 461 275 261 143
EarI CTCTTCn'nnn_
Cuts at: 1353 3160 1353
Size: 1807 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3817 4191 4337 387
Size: 1064 138 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3293 3890 4677 4945 1239
Size: 393 1661 597 787 268 1524
Fragments arranged by size:
1661 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2244 2377 2244
Size: 133 5097
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 4377 1678
Size: 2699 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 3871 3903 3945
Size: 32 135 32 664 32 1603 32 42
Cuts at: 3945 3977 1373
Size: 32 2626
Fragments arranged by size:
2626 1603 664 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2511 2574 2820 669
Size: 988 281 573 63 246 3079
Fragments arranged by size:
3079 988 573 281 246 63
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2511 2820 2511
Size: 309 4921
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2646 3323 4471 4716 1606
Size: 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 5230
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PfoI T'CCnGG_A
Cuts at: 2409 2409
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 2377 2244
Size: 133 5097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SanDI GG'GwC_CC
Cuts at: 2244 2244
Size: 5230
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2669 513
Size: 552 939 244 421 3074
Fragments arranged by size:
3074 939 552 421 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3476 5188
Size: 274 80 33 51 1443 117 1382 1712
Cuts at: 5188 96
Size: 138
Fragments arranged by size:
1712 1443 1382 274 138 117 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI