(Circular) (Six-base) MAPSORT of: pFN21AB4000.seq Check: 3305 from: 1 to: 5347 pFN21AB4000 5347 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3157 278 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2690 1937 Size: 753 4594 Acc65I G'GTAC_C Cuts at: 2668 2668 Size: 5347 AclI AA'CG_TT Cuts at: 1563 3474 3847 1563 Size: 1911 373 3063 Fragments arranged by size: 3063 1911 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3410 4794 5062 1632 Size: 1778 1384 268 1917 Fragments arranged by size: 1917 1778 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5150 AflIII A'CryG_T Cuts at: 1203 4246 5223 1203 Size: 3043 977 1327 Fragments arranged by size: 3043 1327 977 AgeI A'CCGG_T Cuts at: 4178 4178 Size: 5347 AhdI GACnn_n'nnGTC Cuts at: 4076 4076 Size: 5347 AleI CACnn'nnGTG Cuts at: 1166 2285 1166 Size: 1119 4228 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5347 AlwNI CAG_nnn'CTG Cuts at: 2034 2186 2634 3090 4662 2034 Size: 152 448 456 1572 2719 Fragments arranged by size: 2719 1572 456 448 152 ApaLI G'TGCA_C Cuts at: 3404 4560 3404 Size: 1156 4191 ApoI r'AATT_y Cuts at: 1493 2656 2882 3034 5143 1493 Size: 1163 226 152 2109 1697 Fragments arranged by size: 2109 1697 1163 226 152 AseI AT'TA_AT Cuts at: 160 1032 3901 160 Size: 872 2869 1606 Fragments arranged by size: 2869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 AvaI C'yCGr_G Cuts at: 1870 1943 2354 2672 1870 Size: 73 411 318 4545 Fragments arranged by size: 4545 411 318 73 BamHI G'GATC_C Cuts at: 2677 3044 2677 Size: 367 4980 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2668 4123 618 Size: 325 217 627 306 575 1455 1842 Fragments arranged by size: 1842 1455 627 575 325 306 217 BanII G_rGCy'C Cuts at: 1391 2361 2666 1391 Size: 970 305 4072 Fragments arranged by size: 4072 970 305 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4376 BbvCI CC'TCA_GC Cuts at: 2258 2258 Size: 5347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3538 3572 1903 Size: 34 1601 34 3678 Fragments arranged by size: 3678 1601 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3239 4449 3239 Size: 1210 4137 BclI T'GATC_A Cuts at: 1548 1548 Size: 5347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3958 136 Size: 107 122 71 631 605 2286 1525 Fragments arranged by size: 2286 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5342 5342 Size: 5347 BlpI GC'TnA_GC Cuts at: 2764 2764 Size: 5347 Bme1580I G_kGCm'C Cuts at: 1790 2160 3408 4564 1790 Size: 370 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5347 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 4036 442 Size: 416 53 83 399 24 1661 958 1753 Fragments arranged by size: 1753 1661 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5347 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4007 1239 Size: 2768 2579 Bpu10I CC'TnA_GC Cuts at: 2258 2258 Size: 5347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 3472 4337 4635 2345 Size: 1127 865 298 3057 Fragments arranged by size: 3057 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 4010 882 Size: 617 1056 1455 2219 Fragments arranged by size: 2219 1455 1056 617 BsaAI yAC'GTr Cuts at: 493 1204 2155 2380 3138 493 Size: 711 951 225 758 2702 Fragments arranged by size: 2702 951 758 711 225 BsaBI GATnn'nnATC Cuts at: 5341 5341 Size: 5347 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 3154 3536 4171 Size: 53 83 186 936 603 1018 382 635 Cuts at: 4171 275 Size: 1451 Fragments arranged by size: 1451 1018 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3780 4178 4452 4599 5010 3780 Size: 398 274 147 411 4117 Fragments arranged by size: 4117 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5122 Fragments arranged by size: 5122 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5347 BseYI C'CCAG_C Cuts at: 4550 5046 4550 Size: 496 4851 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4774 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2505 2797 3558 4586 1540 Size: 275 186 504 292 761 1028 2301 Fragments arranged by size: 2301 1028 761 504 292 275 186 BsiHKAI G_wGCw'C Cuts at: 2361 2512 3408 3493 4564 2361 Size: 151 896 85 1071 3144 Fragments arranged by size: 3144 1071 896 151 85 BsmI GAATG_Cn' Cuts at: 2858 2951 2858 Size: 93 5254 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2361 2512 2666 3408 3493 4564 Size: 399 370 201 151 154 742 85 1071 Cuts at: 4564 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 370 201 154 151 85 BspEI T'CCGG_A Cuts at: 5010 5010 Size: 5347 BspHI T'CATG_A Cuts at: 3236 4966 3236 Size: 1730 3617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2702 844 Size: 1273 585 3489 Fragments arranged by size: 3489 1273 585 BsrBI CCG'CTC Cuts at: 1394 1823 3234 1394 Size: 429 1411 3507 Fragments arranged by size: 3507 1411 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3842 4016 65 Size: 1183 2594 174 1396 Fragments arranged by size: 2594 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3991 4178 1700 Size: 110 2181 187 2869 Fragments arranged by size: 2869 2181 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5347 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5347 BssSI C'ACGA_G Cuts at: 3407 4419 3407 Size: 1012 4335 BstAPI GCAn_nnn'nTGC Cuts at: 794 2212 794 Size: 1418 3929 BstBI TT'CG_AA Cuts at: 4151 4151 Size: 5347 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5347 BstYI r'GATC_y Cuts at: 1972 2677 3044 3429 3446 4898 4984 4996 5342 Size: 705 367 385 17 1452 86 12 346 Cuts at: 5342 1972 Size: 1977 Fragments arranged by size: 1977 1452 705 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2097 2232 513 Size: 552 1032 135 3628 Fragments arranged by size: 3628 1032 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4608 Fragments arranged by size: 4608 683 56 BtsI GCAGTG_nn' Cuts at: 2863 3657 3677 2863 Size: 794 20 4533 Fragments arranged by size: 4533 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2149 2184 529 Size: 35 1585 35 3692 Fragments arranged by size: 3692 1585 35 35 DraI TTT'AAA Cuts at: 2651 2651 Size: 5347 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4354 809 Size: 507 3038 1802 Fragments arranged by size: 3038 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2794 3683 5312 Size: 54 1425 50 275 18 964 889 1629 Cuts at: 5312 8 Size: 43 Fragments arranged by size: 1629 1425 964 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2794 1537 Size: 275 982 4090 Fragments arranged by size: 4090 982 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3277 1353 Size: 1924 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3934 4308 4454 387 Size: 1064 138 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2359 2359 Size: 5347 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3410 4007 4794 5062 1239 Size: 393 1778 597 787 268 1524 Fragments arranged by size: 1778 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2350 2400 2350 Size: 50 5297 EcoRI G'AATT_C Cuts at: 2656 3034 2656 Size: 378 4969 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2227 2227 Size: 5347 FspI TGC'GCA Cuts at: 3853 3853 Size: 5347 HaeII r_GCGC'y Cuts at: 1678 4494 1678 Size: 2816 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3988 4020 4062 4094 1373 Size: 32 135 32 2416 32 42 32 2626 Fragments arranged by size: 2626 2416 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2691 2937 669 Size: 988 281 753 246 3079 Fragments arranged by size: 3079 988 753 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5347 HpaI GTT'AAC Cuts at: 2937 2937 Size: 5347 KpnI G_GTAC'C Cuts at: 2672 2672 Size: 5347 MfeI C'AATT_G Cuts at: 2946 2946 Size: 5347 MluI A'CGCG_T Cuts at: 5223 5223 Size: 5347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4461 4645 1305 Size: 3156 184 2007 Fragments arranged by size: 3156 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3525 Fragments arranged by size: 3525 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3305 3664 3823 518 Size: 648 1119 1020 359 159 2042 Fragments arranged by size: 2042 1119 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2234 2257 2631 2763 3440 4588 4833 Size: 428 200 23 374 132 677 1148 245 Cuts at: 4833 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 428 374 245 200 132 23 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4795 NdeI CA'TA_TG Cuts at: 387 387 Size: 5347 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5347 NotI GC'GGCC_GC Cuts at: 2794 2794 Size: 5347 NspI r_CATG'y Cuts at: 1132 1229 2374 2705 4250 1132 Size: 97 1145 331 1545 2229 Fragments arranged by size: 2229 1545 1145 331 97 PasI CC'CwG_GG Cuts at: 1348 2144 1348 Size: 796 4551 PciI A'CATG_T Cuts at: 4246 4246 Size: 5347 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5347 PfoI T'CCnGG_A Cuts at: 2337 2337 Size: 5347 PmeI GTTT'AAAC Cuts at: 2651 2651 Size: 5347 PmlI CAC'GTG Cuts at: 2380 2380 Size: 5347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3416 3448 4929 4961 3416 Size: 32 1481 32 3802 Fragments arranged by size: 3802 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2350 2400 2350 Size: 50 5297 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5347 PsiI TTA'TAA Cuts at: 2917 2917 Size: 5347 PstI C_TGCA'G Cuts at: 830 2045 2699 830 Size: 1215 654 3478 Fragments arranged by size: 3478 1215 654 PvuI CG_AT'CG Cuts at: 2001 2505 2001 Size: 504 4843 PvuII CAG'CTG Cuts at: 2034 2257 2631 2034 Size: 223 374 4750 Fragments arranged by size: 4750 374 223 SacI G_AGCT'C Cuts at: 2361 2361 Size: 5347 SacII CC_GC'GG Cuts at: 2235 2235 Size: 5347 SalI G'TCGA_C Cuts at: 1936 2689 1936 Size: 753 4594 SanDI GG'GwC_CC Cuts at: 2350 2400 2350 Size: 50 5297 SbfI CC_TGCA'GG Cuts at: 2699 2699 Size: 5347 ScaI AGT'ACT Cuts at: 3595 5307 3595 Size: 1712 3635 SfcI C'TryA_G Cuts at: 826 1045 2041 2695 3830 4511 4702 826 Size: 219 996 654 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 681 654 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5347 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5347 SmaI CCC'GGG Cuts at: 1872 2674 1872 Size: 802 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 3451 4352 4614 820 Size: 197 926 417 1091 901 262 1553 Fragments arranged by size: 1553 1091 926 901 417 262 197 SnaBI TAC'GTA Cuts at: 493 3138 493 Size: 2645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5347 SphI G_CATG'C Cuts at: 1132 2374 2705 1132 Size: 1242 331 3774 Fragments arranged by size: 3774 1242 331 SspI AAT'ATT Cuts at: 5 52 3271 5 Size: 47 3219 2081 Fragments arranged by size: 3219 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5347 StyI C'CwwG_G Cuts at: 513 1065 2433 2786 513 Size: 552 1368 353 3074 Fragments arranged by size: 3074 1368 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 3391 3544 3729 4174 325 Size: 1255 1794 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3593 5305 96 Size: 274 80 33 51 1443 1616 1712 138 Fragments arranged by size: 1712 1616 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5307 5339 5307 Size: 32 5315 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5347 XbaI T'CTAG_A Cuts at: 2683 2683 Size: 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5347 XmaI C'CCGG_G Cuts at: 1870 2672 1870 Size: 802 4545 XmnI GAAnn'nnTTC Cuts at: 3476 3476 Size: 5347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3155 276 Size: 53 83 186 936 1621 2468 Fragments arranged by size: 2468 1621 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI