Restriction map for
pFN21AB3911
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FseI FspI HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MluI MroI MspI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 96 CGCG CG^CG
Alw44I 63 GTGCAC G^TGCAC
ApaLI 63 GTGCAC G^TGCAC
Asp700I 606 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 63 GTGCAC G[AT]GC[AT]^C
AspI 574 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 566 GGTCC G^G[AT]CC
BalI 951 TGGCCA TGG^CCA
BamHI 225 GGATCC G^GATCC
BbsI 1209 GAAGAC GAAGAC(2/6)
BclI 795 TGATCA T^GATCA
BmyI 63 GTGCAC G[AGT]GC[ACT]^C
BpmI 720 CTGGAG CTGGAG(16/14)
BpuAI 1209 GAAGAC GAAGAC(2/6)
BseRI 19 GAGGAG GAGGAG(10/8)
BsiHKAI 63 GTGCAC G[AT]GC[AT]^C
Bsp1286I 63 GTGCAC G[AGT]GC[ACT]^C
BspHI 195 TCATGA T^CATGA
BspMI 345 ACCTGC ACCTGC(4/8)
BstAPI 969 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 96 CGCG CG^CG
BstYI 225 GGATCC [GA]^GATC[CT]
Cac8I 99 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 566 GGTCC G^G[ACGT]CC
DraI 150 TTTAAA TTT^AAA
DsaI 1293 CCATGG C^C[GA][CT]GG
EaeI 951 TGGCCA [CT]^GGCC[GA]
EcoRI 610 GAATTC G^AATTC
EcoRV 214 GATATC GAT^ATC
FauI 696 GCGGG CCCGC(4/6)
FbaI 795 TGATCA T^GATCA
HaeII 894 AGCGCC [GA]GCGC^[CT]
HgaI 1000 GACGC GACGC(5/10)
MfeI 791 CAATTG C^AATTG
MflI 225 GGATCC [GA]^GATC[CT]
MluNI 951 TGGCCA TGG^CCA
MscI 951 TGGCCA TGG^CCA
MslI 1070 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 791 CAATTG C^AATTG
MvnI 96 CGCG CG^CG
NcoI 1293 CCATGG C^CATGG
PaeR7I 1004 CTCGAG C^TCGAG
PflFI 574 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 557 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 195 TCATGA T^CATGA
Sau96I 566 GGTCC G^G[ACGT]CC
TaqI 1005 TCGA T^CGA
TfiI 394 GAATC G^A[AT]TC
Tth111I 574 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1005 TCGA T^CGA
Van91I 557 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1004 CTCGAG C^TCGAG
XhoII 225 GGATCC [GA]^GATC[CT]
XmnI 606 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 704 CTCGGG C^[CT]CG[GA]G
1004 CTCGAG
BciVI 542 GGATAC GTATCC(6/5)
1222 GGATAC
BfaI 163 CTAG C^TAG
979 CTAG
BsgI 503 CTGCAC GTGCAG(16/14)
1229 GTGCAG
BsiYI 557 CCATACAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTGCATTGGG
BslI 557 CCATACAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
582 CCTGCATTGGG
BsmAI 1007 GAGAC GTCTC(1/5)
671 GTCTC
BsmFI 1080 GGGAC GGGAC(10/14)
1151 GGGAC
BsoBI 704 CTCGGG C^[CT]CG[GA]G
1004 CTCGAG
BssKI 117 CCAGG ^CC[ACGT]GG
928 CCTGG
BstF5I 327 CATCC GGATG(2/0)
189 GGATG
BstNI 117 CCAGG CC^[AT]GG
928 CCTGG
CfoI 97 GCGC GCG^C
895 GCGC
DdeI 280 CTTAG C^T[ACGT]AG
1120 CTCAG
EcoRII 117 CCAGG ^CC[AT]GG
928 CCTGG
FokI 327 CATCC GGATG(9/13)
189 GGATG
HaeIII 120 GGCC GG^CC
952 GGCC
HhaI 97 GCGC GCG^C
895 GCGC
HinP1I 97 GCGC G^CGC
895 GCGC
MaeI 163 CTAG C^TAG
979 CTAG
MvaI 117 CCAGG CC^[AT]GG
928 CCTGG
NlaIV 225 GGATCC GG[ACGT]^[ACGT]CC
699 GGTTCC
PleI 1212 GACTC GAGTC(4/5)
723 GAGTC
PvuII 101 CAGCTG CAG^CTG
220 CAGCTG
ScrFI 117 CCAGG CC^[ACGT]GG
928 CCTGG
SfcI 175 CTACAG C^T[GA][CT]AG
516 CTATAG
SmlI 422 CTCAAG C^T[CT][GA]AG
1004 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI Bsp1407I BsrGI
BsrI DpnII EcoT14I Fnu4HI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MspA1I MwoI NdeII
NlaIII RsaI Sau3AI SfaNI SspBI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI