(Circular) (Six-base) MAPSORT of: pFN21AB3911.seq Check: 9894 from: 1 to: 6067 pFN21AB3911 6067 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2466 4130 5514 5782 1632 Size: 834 1664 1384 268 1917 Fragments arranged by size: 1917 1664 1384 834 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3810 5382 3810 Size: 1572 4495 ApaLI G'TGCA_C Cuts at: 2068 4124 5280 2068 Size: 2056 1156 2855 Fragments arranged by size: 2855 2056 1156 ApoI r'AATT_y Cuts at: 1493 2205 2615 2718 3044 3376 3602 3754 5863 Size: 712 410 103 326 332 226 152 2109 Cuts at: 5863 1493 Size: 1697 Fragments arranged by size: 2109 1697 712 410 332 326 226 152 103 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2709 3009 3392 1870 Size: 73 766 300 383 4545 Fragments arranged by size: 4545 766 383 300 73 BamHI G'GATC_C Cuts at: 2230 3397 3764 2230 Size: 1167 367 4533 Fragments arranged by size: 4533 1167 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3386 1391 Size: 1995 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3221 928 Size: 971 1322 3774 Fragments arranged by size: 3774 1322 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2541 3221 3959 5169 2541 Size: 680 738 1210 3439 Fragments arranged by size: 3439 1210 738 680 BclI T'GATC_A Cuts at: 1548 2800 1548 Size: 1252 4815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2072 4128 5284 1790 Size: 282 2056 1156 2573 Fragments arranged by size: 2573 2056 1156 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2746 4727 1239 Size: 1507 1981 2579 Fragments arranged by size: 2579 1981 1507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2412 4192 5057 5355 2412 Size: 1780 865 298 3124 Fragments arranged by size: 3124 1780 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4730 882 Size: 617 3231 2219 Fragments arranged by size: 3231 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3874 4256 4891 275 Size: 53 83 186 936 2341 382 635 1451 Fragments arranged by size: 2341 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5842 Fragments arranged by size: 5842 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 1186 Size: 853 5214 BseYI C'CCAG_C Cuts at: 3309 5270 5766 3309 Size: 1961 496 3610 Fragments arranged by size: 3610 1961 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2493 3255 1163 Size: 573 757 762 3975 Fragments arranged by size: 3975 762 757 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 4128 4213 5284 2072 Size: 2056 85 1071 2855 Fragments arranged by size: 2855 2056 1071 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 3386 4128 4213 5284 1391 Size: 399 282 1314 742 85 1071 2174 Fragments arranged by size: 2174 1314 1071 742 399 282 85 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 2200 3956 5686 2200 Size: 1756 1730 2581 Fragments arranged by size: 2581 1756 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2359 3422 844 Size: 1515 1063 3489 Fragments arranged by size: 3489 1515 1063 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2278 2290 3192 96 Size: 2182 12 902 2971 Fragments arranged by size: 2971 2182 902 12 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 2980 794 Size: 2186 3881 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2230 3397 3764 4149 4166 5618 5704 5716 Size: 258 1167 367 385 17 1452 86 12 Cuts at: 5716 6062 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1167 385 367 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3298 513 Size: 552 2233 3282 Fragments arranged by size: 3282 2233 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 2157 3371 2157 Size: 1214 4853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2956 3514 4403 Size: 54 1425 50 275 18 1126 558 889 Cuts at: 4403 6032 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1126 889 558 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2466 2746 4130 4727 5514 5782 1239 Size: 393 834 280 1384 597 787 268 1524 Fragments arranged by size: 1524 1384 834 787 597 393 280 268 EcoRI G'AATT_C Cuts at: 2615 3376 3754 2615 Size: 761 378 4928 Fragments arranged by size: 4928 761 378 EcoRV GAT'ATC Cuts at: 2221 2221 Size: 6067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2429 2558 2429 Size: 129 5938 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2903 5214 1678 Size: 1225 2311 2531 Fragments arranged by size: 2531 2311 1225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2912 2944 4708 4740 4782 Size: 32 135 32 1340 32 1764 32 42 Cuts at: 4782 4814 1373 Size: 32 2626 Fragments arranged by size: 2626 1764 1340 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 2796 3666 2796 Size: 870 5197 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2989 5181 5365 1305 Size: 1684 2192 184 2007 Fragments arranged by size: 2192 2007 1684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2958 10 Size: 54 1425 343 1126 3119 Fragments arranged by size: 3119 1425 1126 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3079 4025 4384 4543 518 Size: 648 1913 946 359 159 2042 Fragments arranged by size: 2042 1913 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 2227 2701 3239 3483 4160 5308 5553 Size: 502 119 474 538 244 677 1148 245 Cuts at: 5553 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 538 502 474 245 244 119 NcoI C'CATG_G Cuts at: 513 1065 3298 513 Size: 552 2233 3282 Fragments arranged by size: 3282 2233 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 2568 1108 Size: 1460 4607 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 2864 3637 2864 Size: 773 5294 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2163 2195 2163 Size: 32 6035 PstI C_TGCA'G Cuts at: 830 3419 830 Size: 2589 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2108 2227 2108 Size: 119 5948 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2180 2521 3415 4550 5231 5422 826 Size: 219 1135 341 894 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 894 681 341 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2427 3009 4171 5072 5334 820 Size: 197 926 484 582 1162 901 262 1553 Fragments arranged by size: 1553 1162 926 901 582 484 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2254 2308 2542 3298 3506 513 Size: 552 1189 54 234 756 208 3074 Fragments arranged by size: 3074 1189 756 552 234 208 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 2642 4094 4111 4264 4449 4894 Size: 1255 1004 58 1452 17 153 185 445 Cuts at: 4894 325 Size: 1498 Fragments arranged by size: 1498 1452 1255 1004 445 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 2290 2890 Size: 274 80 33 51 1443 301 12 600 Cuts at: 2890 3192 4313 6025 96 Size: 302 1121 1712 138 Fragments arranged by size: 1712 1443 1121 600 302 301 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 2582 1316 Size: 1266 4801 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 3009 1943 Size: 1066 5001 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2615 4196 2615 Size: 1581 4486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI