Restriction map for
pFN21AB3881
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvrII BalI BanI BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspLU11I BspMI BsrFI BssSI
BstBI BstEII BstPI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MseI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tru9I Tsp509I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 649 GTATAC GT^[AC][GT]AC
AccIII 596 TCCGGA T^CCGGA
AcyI 1012 GACGCC G[GA]^CG[CT]C
AspI 340 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 848 TGCGCA TGC^GCA
BanII 978 GAGCCC G[GA]GC[CT]^C
BbvCI 232 GCTGAGG CCTCAGC(-5/-2)
BcgI 454 CGAGTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 70 TACGTG [CT]AC^GT[GA]
BsaHI 1012 GACGCC G[GA]^CG[CT]C
BseAI 596 TCCGGA T^CCGGA
BsmAI 1018 GAGAC GTCTC(1/5)
Bsp1407I 206 TGTACA T^GTACA
BspEI 596 TCCGGA T^CCGGA
BspHI 521 TCATGA T^CATGA
BsrGI 206 TGTACA T^GTACA
Bst1107I 649 GTATAC GTA^TAC
BstZ17I 649 GTATAC GTA^TAC
DrdI 574 GACTGTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 502 CGGCCA [CT]^GGCC[GA]
EarI 190 CTCTTC CTCTTC(1/4)
EcoT14I 764 CCAAGG C^C[AT][AT]GG
FauI 774 GCGGG CCCGC(4/6)
FspI 848 TGCGCA TGC^GCA
Hin1I 1012 GACGCC G[GA]^CG[CT]C
HindIII 1027 AAGCTT A^AGCTT
Ksp632I 190 CTCTTC CTCTTC(1/4)
MroI 596 TCCGGA T^CCGGA
NdeI 871 CATATG CA^TATG
PflFI 340 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1075 CTGCAG CTGCA^G
RcaI 521 TCATGA T^CATGA
ScaI 404 AGTACT AGT^ACT
SexAI 95 ACCAGGT A^CC[AT]GGT
SfcI 1075 CTGCAG C^T[GA][CT]AG
SmlI 883 CTCAAG C^T[CT][GA]AG
SphI 1061 GCATGC GCATG^C
SspBI 206 TGTACA T^GTACA
StyI 764 CCAAGG C^C[AT][AT]GG
Tth111I 340 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 614 GGATCC G^GATCC
641 GGATCC
BsaWI 215 ACCGGA [AT]^CCGG[AT]
596 TCCGGA
BsmFI 338 GGGAC GGGAC(10/14)
583 GTCCC
BsrBI 420 GAGCGG CCGCTC(-3/-3)
610 GAGCGG
BsrDI 530 CATTGC GCAATG(2/0)
829 CATTGC
BssHII 722 GCGCGC G^CGCGC
923 GCGCGC
DraIII 271 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
354 CACCGAGTG
HgaI 340 GACGC GACGC(5/10)
1012 GACGC
HincII 346 GTCAAC GT[CT]^[GA]AC
544 GTCAAC
HindII 346 GTCAAC GT[CT]^[GA]AC
544 GTCAAC
MaeIII 668 GTGAC ^GT[ACGT]AC
892 GTGAC
MboII 117 GAAGA GAAGA(8/7)
191 TCTTC
MspA1I 772 CAGCGG C[AC]G^C[GT]G
997 CCGCTG
NspI 854 ACATGC [GA]CATG^[CT]
1061 GCATGC
StuI 9 AGGCCT AGG^CCT
159 AGGCCT
TfiI 261 GATTC G^A[AT]TC
332 GATTC
Tsp45I 668 GTGAC ^GT[GC]AC
892 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI ApaLI AspHI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MflI MnlI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI XhoII