(Circular) (Six-base) MAPSORT of: pFN21AB3881.seq Check: 4463 from: 1 to: 5812 pFN21AB3881 5812 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3155 1937 Size: 718 500 4594 Fragments arranged by size: 4594 718 500 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2630 2738 3875 5259 5527 1632 Size: 698 300 108 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1137 698 300 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 4711 5688 1203 Size: 3508 977 1327 Fragments arranged by size: 3508 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 2321 2759 1166 Size: 1155 438 4219 Fragments arranged by size: 4219 1155 438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2033 2894 1184 Size: 849 861 4102 Fragments arranged by size: 4102 861 849 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 2105 2462 2909 3869 5025 2105 Size: 357 447 960 1156 2892 Fragments arranged by size: 2892 1156 960 447 357 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 2619 2646 3142 3509 2619 Size: 27 496 367 4922 Fragments arranged by size: 4922 496 367 27 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3133 4588 618 Size: 325 217 627 1346 1455 1842 Fragments arranged by size: 1842 1455 1346 627 325 217 BanII G_rGCy'C Cuts at: 1391 2987 3131 1391 Size: 1596 144 4072 Fragments arranged by size: 4072 1596 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4841 BbvCI CC'TCA_GC Cuts at: 2238 2238 Size: 5812 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2448 2482 4003 4037 1903 Size: 34 511 34 1521 34 3678 Fragments arranged by size: 3678 1521 511 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 2109 2466 2913 3873 5029 1790 Size: 319 357 447 960 1156 2573 Fragments arranged by size: 2573 1156 960 447 357 319 BmgBI CAC'GTC Cuts at: 1318 2896 2901 2908 1318 Size: 1578 5 7 4222 Fragments arranged by size: 4222 1578 7 5 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3543 4501 Size: 416 53 83 399 24 1169 957 958 Cuts at: 4501 442 Size: 1753 Fragments arranged by size: 1753 1169 958 957 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2692 2692 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4472 1239 Size: 3233 2579 Bpu10I CC'TnA_GC Cuts at: 2238 2238 Size: 5812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 3937 4802 5100 2873 Size: 1064 865 298 3585 Fragments arranged by size: 3585 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2077 3603 493 Size: 711 873 1526 2702 Fragments arranged by size: 2702 1526 873 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3018 3619 4001 4636 Size: 53 83 186 936 1485 601 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1485 1451 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2220 2601 4245 4643 4917 5064 5475 2220 Size: 381 1644 398 274 147 411 2557 Fragments arranged by size: 2557 1644 411 398 381 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 1178 Size: 30 165 30 1065 30 4492 Fragments arranged by size: 4492 1065 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2306 2642 1186 Size: 1069 51 336 4356 Fragments arranged by size: 4356 1069 336 51 BseYI C'CCAG_C Cuts at: 5015 5511 5015 Size: 496 5316 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2109 2466 2913 3873 3958 5029 2012 Size: 97 357 447 960 85 1071 2795 Fragments arranged by size: 2795 1071 960 447 357 97 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2109 2466 2913 2987 3131 3873 Size: 399 222 97 357 447 74 144 742 Cuts at: 3873 3958 5029 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 357 222 144 97 85 74 BspEI T'CCGG_A Cuts at: 2601 5475 2601 Size: 2874 2938 BspHI T'CATG_A Cuts at: 2526 3701 5431 2526 Size: 1175 1730 2907 Fragments arranged by size: 2907 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3167 844 Size: 2323 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2427 2617 3699 1394 Size: 429 604 190 1082 3507 Fragments arranged by size: 3507 1082 604 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 2534 2833 4307 4481 65 Size: 1183 1286 299 1474 174 1396 Fragments arranged by size: 1474 1396 1286 1183 299 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 2211 96 Size: 2115 3697 BssHII G'CGCG_C Cuts at: 1925 2727 2928 1925 Size: 802 201 4809 Fragments arranged by size: 4809 802 201 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2503 3076 3100 794 Size: 1709 573 24 3506 Fragments arranged by size: 3506 1709 573 24 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstYI r'GATC_y Cuts at: 1972 2598 2619 2646 3142 3509 3894 3911 5363 Size: 626 21 27 496 367 385 17 1452 Cuts at: 5363 5449 5461 5807 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 626 496 385 367 346 86 27 21 17 12 BstZ17I GTA'TAC Cuts at: 2656 2656 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5260 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2551 487 Size: 683 56 1325 3748 Fragments arranged by size: 3748 1325 683 56 BtsI GCAGTG_nn' Cuts at: 2090 2464 2932 3328 4122 4142 2090 Size: 374 468 396 794 20 3760 Fragments arranged by size: 3760 794 468 396 374 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DraIII CAC_nnn'GTG Cuts at: 2281 2364 2281 Size: 83 5729 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2585 4819 809 Size: 507 1269 2234 1802 Fragments arranged by size: 2234 1802 1269 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2507 3259 4148 Size: 54 1425 50 275 18 677 752 889 Cuts at: 4148 5777 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 752 677 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 3742 1353 Size: 848 1541 3423 Fragments arranged by size: 3423 1541 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2630 2738 3875 4472 5259 5527 Size: 393 698 300 108 1137 597 787 268 Cuts at: 5527 1239 Size: 1524 Fragments arranged by size: 1524 1137 787 698 597 393 300 268 108 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2146 2146 Size: 5812 FspI TGC'GCA Cuts at: 2855 4318 2855 Size: 1463 4349 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373 Size: 32 135 32 2881 32 42 32 2626 Fragments arranged by size: 2881 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2353 2551 3156 3402 669 Size: 988 281 415 198 605 246 3079 Fragments arranged by size: 3079 988 605 415 281 246 198 HindIII A'AGCT_T Cuts at: 748 3032 748 Size: 2284 3528 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2321 2759 2876 3770 4129 4288 Size: 648 978 177 438 117 894 359 159 Cuts at: 4288 518 Size: 2042 Fragments arranged by size: 2042 978 894 648 438 359 177 159 117 MspA1I CmG'CkG Cuts at: 1606 2779 3004 3228 3905 5053 5298 1606 Size: 1173 225 224 677 1148 245 2120 Fragments arranged by size: 2120 1173 1148 677 245 225 224 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5260 NdeI CA'TA_TG Cuts at: 387 2877 387 Size: 2490 3322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2863 3070 3170 4715 1132 Size: 97 1634 207 100 1545 2229 Fragments arranged by size: 2229 1634 1545 207 100 97 PasI CC'CwG_GG Cuts at: 1348 2802 1348 Size: 1454 4358 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PfoI T'CCnGG_A Cuts at: 2622 2622 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PstI C_TGCA'G Cuts at: 830 3084 3164 830 Size: 2254 80 3478 Fragments arranged by size: 3478 2254 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 2411 4060 5772 2411 Size: 1649 1712 2451 Fragments arranged by size: 2451 1712 1649 SexAI A'CCwGG_T Cuts at: 2100 3108 2100 Size: 1008 4804 SfcI C'TryA_G Cuts at: 826 1045 3080 3160 4295 4976 5167 826 Size: 219 2035 80 1135 681 191 1471 Fragments arranged by size: 2035 1471 1135 681 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 3916 4817 5079 820 Size: 197 926 945 1028 901 262 1553 Fragments arranged by size: 1553 1028 945 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3070 3170 1132 Size: 1938 100 3774 Fragments arranged by size: 3774 1938 100 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 2016 2166 1517 Size: 499 150 5163 Fragments arranged by size: 5163 499 150 StyI C'CwwG_G Cuts at: 513 1065 2769 3251 513 Size: 552 1704 482 3074 Fragments arranged by size: 3074 1704 552 482 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3839 3856 4009 4194 4639 325 Size: 1255 2259 17 153 185 445 1498 Fragments arranged by size: 2259 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 2409 4058 Size: 274 80 33 51 1443 234 198 1649 Cuts at: 4058 5770 96 Size: 1712 138 Fragments arranged by size: 1712 1649 1443 274 234 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2885 2917 5772 5804 2885 Size: 32 2855 32 2893 Fragments arranged by size: 2893 2855 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2348 1316 Size: 1032 4780 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI