(Circular) (Six-base) MAPSORT of: pFN21AB3878.seq Check: 1817 from: 1 to: 5851 pFN21AB3878 5851 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3066 3066 Size: 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 3914 5298 5566 1632 Size: 928 1354 1384 268 1917 Fragments arranged by size: 1917 1384 1354 928 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2537 2569 2537 Size: 32 5819 AlwNI CAG_nnn'CTG Cuts at: 3594 5166 3594 Size: 1572 4279 ApaI G_GGCC'C Cuts at: 2100 2100 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2099 3176 1870 Size: 73 144 12 1077 4545 Fragments arranged by size: 4545 1077 144 73 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2936 2969 2936 Size: 33 5818 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 4627 618 Size: 325 217 627 1385 1455 1842 Fragments arranged by size: 1842 1455 1385 627 325 217 BanII G_rGCy'C Cuts at: 1391 2100 2355 3170 1391 Size: 709 255 815 4072 Fragments arranged by size: 4072 815 709 255 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2566 2629 928 Size: 971 667 63 4150 Fragments arranged by size: 4150 971 667 63 BbvCI CC'TCA_GC Cuts at: 2145 2531 2145 Size: 386 5465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2777 3743 4953 2777 Size: 966 1210 3675 Fragments arranged by size: 3675 1210 966 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 4462 136 Size: 107 122 71 631 605 382 2408 1525 Fragments arranged by size: 2408 1525 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 2537 2624 3268 2537 Size: 87 644 5120 Fragments arranged by size: 5120 644 87 Bme1580I G_kGCm'C Cuts at: 1790 2100 2925 3912 5068 1790 Size: 310 825 987 1156 2573 Fragments arranged by size: 2573 1156 987 825 310 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2103 3582 4540 Size: 416 53 83 399 24 686 1479 958 Cuts at: 4540 442 Size: 1753 Fragments arranged by size: 1753 1479 958 686 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2518 2518 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 2315 2462 2539 2543 2594 2606 3062 Size: 939 137 147 77 4 51 12 456 Cuts at: 3062 4511 1239 Size: 1449 2579 Fragments arranged by size: 2579 1449 939 456 147 137 77 51 12 4 Bpu10I CC'TnA_GC Cuts at: 2145 2531 2145 Size: 386 5465 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 3976 4841 5139 2648 Size: 1328 865 298 3360 Fragments arranged by size: 3360 1328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2762 4514 882 Size: 617 1263 1752 2219 Fragments arranged by size: 2219 1752 1263 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 3658 4040 4675 Size: 53 83 186 936 1101 1024 382 635 Cuts at: 4675 275 Size: 1451 Fragments arranged by size: 1451 1101 1024 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4462 Fragments arranged by size: 4462 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2018 2172 3022 1186 Size: 832 154 850 4015 Fragments arranged by size: 4015 850 832 154 BseYI C'CCAG_C Cuts at: 2135 5054 5550 2135 Size: 2919 496 2436 Fragments arranged by size: 2919 2436 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2355 2892 3912 3997 5068 2355 Size: 537 1020 85 1071 3138 Fragments arranged by size: 3138 1071 1020 537 85 BsmI GAATG_Cn' Cuts at: 2237 2380 3362 3455 2237 Size: 143 982 93 4633 Fragments arranged by size: 4633 982 143 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2355 2892 2925 3170 3912 3997 Size: 399 310 255 537 33 245 742 85 Cuts at: 3997 5068 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 310 255 245 85 33 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3114 3740 5470 3114 Size: 626 1730 3495 Fragments arranged by size: 3495 1730 626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 2292 2601 2889 3066 3075 3206 844 Size: 1340 108 309 288 177 9 131 3489 Fragments arranged by size: 3489 1340 309 288 177 131 108 9 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 2010 1184 Size: 826 5025 BstXI CCAn_nnnn'nTGG Cuts at: 3106 3106 Size: 5851 BstYI r'GATC_y Cuts at: 1972 2605 3181 3548 3933 3950 5402 5488 5500 Size: 633 576 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 633 576 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2656 3141 513 Size: 552 1591 485 3223 Fragments arranged by size: 3223 1591 552 485 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2404 487 Size: 683 56 1178 3934 Fragments arranged by size: 3934 1178 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3367 4161 4181 2923 Size: 444 794 20 4593 Fragments arranged by size: 4593 794 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816 Size: 54 1425 50 275 18 1468 889 1629 Cuts at: 5816 8 Size: 43 Fragments arranged by size: 1629 1468 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2487 3781 1353 Size: 1134 1294 3423 Fragments arranged by size: 3423 1294 1134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3116 4438 4812 4958 387 Size: 1064 138 1527 1322 374 146 1280 Fragments arranged by size: 1527 1322 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2353 2353 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2315 2462 2539 2543 2560 2594 Size: 393 546 137 147 77 4 17 34 Cuts at: 2594 2606 3062 3914 4511 5298 5566 1239 Size: 12 456 852 597 787 268 1524 Fragments arranged by size: 1524 852 787 597 546 456 393 268 147 137 77 34 17 12 4 EcoNI CCTnn'n_nnAGG Cuts at: 2288 2288 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2198 2293 2497 2722 2198 Size: 95 204 225 5327 Fragments arranged by size: 5327 225 204 95 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 5851 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 4998 1678 Size: 3320 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 3017 3049 4492 Size: 32 135 32 668 32 745 32 1443 Cuts at: 4492 4524 4566 4598 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 745 668 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 2901 4965 5149 1305 Size: 1164 432 2064 184 2007 Fragments arranged by size: 2064 2007 1164 432 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4168 4327 518 Size: 648 2643 359 159 2042 Fragments arranged by size: 2643 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2530 3106 3267 3944 5092 5337 1606 Size: 924 576 161 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 677 576 245 161 NcoI C'CATG_G Cuts at: 513 1065 2656 513 Size: 552 1591 3708 Fragments arranged by size: 3708 1591 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 3209 4754 1132 Size: 97 1980 1545 2229 Fragments arranged by size: 2229 1980 1545 97 PasI CC'CwG_GG Cuts at: 1348 2718 1348 Size: 1370 4481 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 3920 3952 5433 5465 2537 Size: 32 1351 32 1481 32 2923 Fragments arranged by size: 2923 1481 1351 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2497 2722 2293 Size: 204 225 5422 Fragments arranged by size: 5422 225 204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2096 2096 Size: 5851 PstI C_TGCA'G Cuts at: 830 3138 3203 830 Size: 2308 65 3478 Fragments arranged by size: 3478 2308 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2530 2530 Size: 5851 SacI G_AGCT'C Cuts at: 2355 2355 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SanDI GG'GwC_CC Cuts at: 2722 2722 Size: 5851 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 3134 3199 4334 5015 5206 826 Size: 219 2089 65 1135 681 191 1471 Fragments arranged by size: 2089 1471 1135 681 219 191 65 SfiI GGCCn_nnn'nGGCC Cuts at: 2054 2054 Size: 5851 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 2089 2101 3178 1872 Size: 217 12 1077 4545 Fragments arranged by size: 4545 1077 217 12 SmlI C'TyrA_G Cuts at: 820 1017 1943 2663 3955 4856 5118 820 Size: 197 926 720 1292 901 262 1553 Fragments arranged by size: 1553 1292 926 901 720 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SrfI GCCC'GGGC Cuts at: 2101 2101 Size: 5851 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 2385 1517 Size: 868 4983 StyI C'CwwG_G Cuts at: 513 1065 2059 2656 3290 513 Size: 552 994 597 634 3074 Fragments arranged by size: 3074 994 634 597 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 3878 3895 4048 4233 4678 325 Size: 1255 569 1729 17 153 185 445 1498 Fragments arranged by size: 1729 1498 1255 569 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2902 2934 5811 5843 2902 Size: 32 2877 32 2910 Fragments arranged by size: 2910 2877 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2633 1316 Size: 1317 4534 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3010 3010 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 2087 2099 3176 1870 Size: 217 12 1077 4545 Fragments arranged by size: 4545 1077 217 12 XmnI GAAnn'nnTTC Cuts at: 2339 3980 2339 Size: 1641 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfoI SwaI