Restriction map for
pFN21AB3845
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BamHI BbeI BbrPI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI HaeII Hin1I HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AgeI 172 ACCGGT A^CCGGT
AspI 904 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 152 TGCGCA TGC^GCA
BalI 201 TGGCCA TGG^CCA
BbvCI 585 CCTCAGC CCTCAGC(-5/-2)
BcgI 663 GCAAAGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 282 CCGGG CC^[GC]GG
BsiEI 834 CGGCCG CG[GA][CT]^CG
BspHI 575 TCATGA T^CATGA
BspMI 110 ACCTGC ACCTGC(4/8)
BsrDI 615 CATTGC GCAATG(2/0)
BsrFI 172 ACCGGT [GA]^CCGG[CT]
BsrI 213 ACTGG ACTGG(1/-1)
BssSI 506 CTCGTG CACGAG(-5/-1)
CfoI 153 GCGC GCG^C
Cfr10I 172 ACCGGT [GA]^CCGG[CT]
DrdI 502 GACACTCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 847 CCATGG C^C[GA][CT]GG
EagI 834 CGGCCG C^GGCCG
EarI 241 CTCTTC CTCTTC(1/4)
EclXI 834 CGGCCG C^GGCCG
Eco52I 834 CGGCCG C^GGCCG
EcoT14I 847 CCATGG C^C[AT][AT]GG
FspI 152 TGCGCA TGC^GCA
HgaI 31 GACGC GACGC(5/10)
HhaI 153 GCGC GCG^C
HinP1I 153 GCGC G^CGC
HphI 1011 GGTGA GGTGA(8/7)
Ksp632I 241 CTCTTC CTCTTC(1/4)
MluNI 201 TGGCCA TGG^CCA
MscI 201 TGGCCA TGG^CCA
MslI 858 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 282 CCGGG CC^[GC]GG
NcoI 847 CCATGG C^CATGG
PflFI 904 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 379 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 172 ACCGGT A^CCGGT
PvuII 273 CAGCTG CAG^CTG
RcaI 575 TCATGA T^CATGA
SapI 240 GCTCTTC GCTCTTC(1/4)
SphI 773 GCATGC GCATG^C
StyI 847 CCATGG C^C[AT][AT]GG
Tth111I 904 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 379 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 38 CCATGTCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanI 51 GGTGCC G^G[CT][GA]CC
78 GGTGCC
BbsI 159 GAAGAC GAAGAC(2/6)
168 GAAGAC
BbvI 34 GCAGC GCAGC(8/12)
518 GCTGC
BpuAI 159 GAAGAC GAAGAC(2/6)
168 GAAGAC
BsaI 19 GAGACC GGTCTC(1/5)
389 GAGACC
BsaWI 172 ACCGGT [AT]^CCGG[AT]
1008 TCCGGT
BstYI 293 AGATCC [GA]^GATC[CT]
564 GGATCT
Cac8I 72 GCCAGC GC[ACGT]^[ACGT]GC
773 GCATGC
EaeI 201 TGGCCA [CT]^GGCC[GA]
834 CGGCCG
EcoNI 459 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
585 CCTCAGCAAGG
EcoRI 655 GAATTC G^AATTC
700 GAATTC
HincII 232 GTCAAC GT[CT]^[GA]AC
472 GTCAAC
HindII 232 GTCAAC GT[CT]^[GA]AC
472 GTCAAC
MflI 293 AGATCC [GA]^GATC[CT]
564 GGATCT
MspA1I 74 CAGCGG C[AC]G^C[GT]G
273 CAGCTG
NspI 327 GCATGT [GA]CATG^[CT]
773 GCATGC
SmlI 568 CTTGAG C^T[CT][GA]AG
934 CTCAAG
TseI 34 GCAGC G^C[AT]GC
518 GCTGC
Tsp45I 797 GTGAC ^GT[GC]AC
943 GTGAC
XhoII 293 AGATCC [GA]^GATC[CT]
564 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstXI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI TaqI
Tsp509I TspRI TthHB8I