(Circular) (Six-base) MAPSORT of: pFN21AB3845.seq Check: 8970 from: 1 to: 5755 pFN21AB3845 5755 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2486 3818 5202 5470 1632 Size: 854 1332 1384 268 1917 Fragments arranged by size: 1917 1384 1332 854 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 2177 4586 2177 Size: 2409 3346 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2561 2593 2561 Size: 32 5723 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2660 2705 2723 3064 3290 3442 5551 1493 Size: 1167 45 18 341 226 152 2109 1697 Fragments arranged by size: 2109 1697 1167 341 226 152 45 18 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2083 3076 4531 618 Size: 325 217 627 269 27 993 1455 1842 Fragments arranged by size: 1842 1455 993 627 325 269 217 27 BanII G_rGCy'C Cuts at: 1391 2019 2067 2427 3074 1391 Size: 628 48 360 647 4072 Fragments arranged by size: 4072 647 628 360 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2180 928 Size: 971 272 9 4503 Fragments arranged by size: 4503 971 272 9 BbvCI CC'TCA_GC Cuts at: 2591 2591 Size: 5755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2657 2691 3946 3980 1903 Size: 34 720 34 1255 34 3678 Fragments arranged by size: 3678 1255 720 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2088 3816 4972 1790 Size: 298 1728 1156 2573 Fragments arranged by size: 2573 1728 1156 298 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 3047 3486 Size: 416 53 83 399 24 811 819 439 Cuts at: 3486 4444 442 Size: 958 1753 Fragments arranged by size: 1753 958 819 811 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2260 2666 2915 4415 1239 Size: 1021 406 249 1500 2579 Fragments arranged by size: 2579 1500 1021 406 249 Bpu10I CC'TnA_GC Cuts at: 2061 2073 2591 2625 2061 Size: 12 518 34 5191 Fragments arranged by size: 5191 518 34 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 2924 3880 4745 5043 2594 Size: 330 956 865 298 3306 Fragments arranged by size: 3306 956 865 330 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 2388 4418 882 Size: 617 519 370 2030 2219 Fragments arranged by size: 2219 2030 617 519 370 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2177 3013 4188 4586 4860 5007 5418 2177 Size: 836 1175 398 274 147 411 2514 Fragments arranged by size: 2514 1175 836 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 1178 Size: 30 165 30 1236 30 4264 Fragments arranged by size: 4264 1236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2486 2663 1186 Size: 1120 180 177 4278 Fragments arranged by size: 4278 1120 180 177 BseYI C'CCAG_C Cuts at: 2119 2418 4958 5454 2119 Size: 299 2540 496 2420 Fragments arranged by size: 2540 2420 496 299 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3205 3966 4994 1540 Size: 275 186 841 363 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 363 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2067 2088 2427 3074 3816 3901 Size: 399 229 48 21 339 647 742 85 Cuts at: 3901 4972 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 647 399 339 229 85 48 21 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 2580 3644 5374 2580 Size: 1064 1730 2961 Fragments arranged by size: 2961 1730 1064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 3110 844 Size: 1280 986 3489 Fragments arranged by size: 3489 1280 986 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2619 4250 4424 65 Size: 1183 1371 1631 174 1396 Fragments arranged by size: 1631 1396 1371 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2177 4399 4586 1700 Size: 110 367 2222 187 2869 Fragments arranged by size: 2869 2222 367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 2511 3815 4827 2511 Size: 1304 1012 3439 Fragments arranged by size: 3439 1304 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2119 2311 2644 2822 2119 Size: 192 333 178 5052 Fragments arranged by size: 5052 333 192 178 BstYI r'GATC_y Cuts at: 1972 2298 2569 3085 3452 3837 3854 5306 5392 Size: 326 271 516 367 385 17 1452 86 Cuts at: 5392 5404 5750 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 516 385 367 346 326 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2852 513 Size: 552 1787 3416 Fragments arranged by size: 3416 1787 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2467 487 Size: 683 56 1241 3775 Fragments arranged by size: 3775 1241 683 56 BtsI GCAGTG_nn' Cuts at: 2518 3271 4065 4085 2518 Size: 753 794 20 4188 Fragments arranged by size: 4188 794 753 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2513 4762 809 Size: 507 1197 2249 1802 Fragments arranged by size: 2249 1802 1197 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2839 3202 Size: 54 1425 50 275 18 376 633 363 Cuts at: 3202 4091 5720 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 633 376 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 3202 1537 Size: 275 1027 363 4090 Fragments arranged by size: 4090 1027 363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 3685 1353 Size: 899 1433 3423 Fragments arranged by size: 3423 1433 899 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 2486 2666 2915 3818 4415 5202 Size: 393 628 226 180 249 903 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 903 787 628 597 393 268 249 226 180 EcoNI CCTnn'n_nnAGG Cuts at: 2468 2594 2468 Size: 126 5629 EcoO109I rG'GnC_Cy Cuts at: 2833 2922 2935 2833 Size: 89 13 5653 Fragments arranged by size: 5653 89 13 EcoRI G'AATT_C Cuts at: 2660 2705 3064 3442 2660 Size: 45 359 378 4973 Fragments arranged by size: 4973 378 359 45 FspI TGC'GCA Cuts at: 2159 4261 2159 Size: 2102 3653 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2385 2417 2639 2671 2898 Size: 32 135 32 813 32 222 32 227 Cuts at: 2898 2930 4396 4428 4470 4502 1373 Size: 32 1466 32 42 32 2626 Fragments arranged by size: 2626 1466 813 227 222 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 2479 3099 3345 669 Size: 988 281 301 240 620 246 3079 Fragments arranged by size: 3079 988 620 301 281 246 240 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 4869 5053 1305 Size: 831 2733 184 2007 Fragments arranged by size: 2733 2007 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2208 10 Size: 54 1425 343 376 3557 Fragments arranged by size: 3557 1425 376 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2867 3713 4072 4231 518 Size: 648 1701 846 359 159 2042 Fragments arranged by size: 2042 1701 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 2280 3171 3848 4996 5241 1606 Size: 475 199 891 677 1148 245 2120 Fragments arranged by size: 2120 1148 891 677 475 245 199 NcoI C'CATG_G Cuts at: 513 1065 2852 513 Size: 552 1787 3416 Fragments arranged by size: 3416 1787 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2336 2782 3113 4658 1132 Size: 97 1107 446 331 1545 2229 Fragments arranged by size: 2229 1545 1107 446 331 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2390 1108 Size: 1282 4473 PfoI T'CCnGG_A Cuts at: 2931 2931 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 2922 2935 2833 Size: 89 13 5653 Fragments arranged by size: 5653 89 13 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2444 2476 2444 Size: 32 5723 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2280 2280 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SapI GCTCTTCn'nnn_ Cuts at: 2252 2252 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 3103 4238 4919 5110 826 Size: 219 2058 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 2939 3859 4760 5022 820 Size: 197 926 630 366 920 901 262 1553 Fragments arranged by size: 1553 926 920 901 630 366 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2782 3113 1132 Size: 1650 331 3774 Fragments arranged by size: 3774 1650 331 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2071 2273 3006 1517 Size: 554 202 733 4266 Fragments arranged by size: 4266 733 554 202 StyI C'CwwG_G Cuts at: 513 1065 2852 3194 513 Size: 552 1787 342 3074 Fragments arranged by size: 3074 1787 552 342 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2436 4001 5713 Size: 274 80 33 51 1443 459 1565 1712 Cuts at: 5713 96 Size: 138 Fragments arranged by size: 1712 1565 1443 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2943 2975 5715 5747 2943 Size: 32 2740 32 2951 Fragments arranged by size: 2951 2740 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2912 1316 Size: 1596 4159 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2050 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI