Restriction map for
pFN21AB3754
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BlnI BsaAI BsaHI BsiEI
BsiWI BsmI BsoBI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 360 TCCGGA T^CCGGA
AflII 1656 CTTAAG C^TTAAG
AocI 1058 CCTCAGG CC^T[ACGT]AGG
AspI 1957 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1180 CCTCAGC CCTCAGC(-5/-2)
BfrI 1656 CTTAAG C^TTAAG
BglII 75 AGATCT A^GATCT
BlpI 1249 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1249 GCTGAGC GC^T[ACGT]AGC
BsaI 1462 GGTCTC GGTCTC(1/5)
BseAI 360 TCCGGA T^CCGGA
BsgI 1927 GTGCAG GTGCAG(16/14)
BsmBI 1169 CGTCTC CGTCTC(1/5)
Bsp1407I 1620 TGTACA T^GTACA
BspDI 1216 ATCGAT AT^CGAT
BspEI 360 TCCGGA T^CCGGA
BspHI 1466 TCATGA T^CATGA
BspMI 882 ACCTGC ACCTGC(4/8)
BsrBI 1503 CCGCTC CCGCTC(-3/-3)
BsrDI 182 CATTGC GCAATG(2/0)
BsrGI 1620 TGTACA T^GTACA
BstXI 1355 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1058 CCTCAGG CC^T[ACGT]AGG
CelII 1249 GCTGAGC GC^T[ACGT]AGC
ClaI 1216 ATCGAT AT^CGAT
DraII 549 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1058 CCTCAGG CC^T[ACGT]AGG
EcoO109I 549 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1725 GAATTC G^AATTC
EcoT22I 902 ATGCAT ATGCA^T
MroI 360 TCCGGA T^CCGGA
NdeI 162 CATATG CA^TATG
NsiI 902 ATGCAT ATGCA^T
PflFI 1957 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 549 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1679 CTGCAG CTGCA^G
RcaI 1466 TCATGA T^CATGA
SacI 1981 GAGCTC GAGCT^C
SexAI 152 ACCTGGT A^CC[AT]GGT
SspBI 1620 TGTACA T^GTACA
Tth111I 1957 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 473 CCAGTGGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 230 ACGTGT A^C[GA][CT]GT
866 ACACGT
BanII 68 GGGCTC G[GA]GC[CT]^C
1981 GAGCTC
BbsI 44 GAAGAC GAAGAC(2/6)
709 GTCTTC
BpuAI 44 GAAGAC GAAGAC(2/6)
709 GTCTTC
BsaBI 76 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1912 GATGCAGATC
BsrFI 826 GCCGGT [GA]^CCGG[CT]
1103 ACCGGC
BsrI 473 CCAGT ACTGG(1/-1)
1452 CCAGT
CfoI 1389 GCGC GCG^C
1831 GCGC
Cfr10I 826 GCCGGT [GA]^CCGG[CT]
1103 ACCGGC
EarI 677 CTCTTC CTCTTC(1/4)
1164 CTCTTC
EcoT14I 32 CCATGG C^C[AT][AT]GG
369 CCATGG
HaeIII 367 GGCC GG^CC
1532 GGCC
HgaI 1578 GACGC GACGC(5/10)
1558 GCGTC
HhaI 1389 GCGC GCG^C
1831 GCGC
HinP1I 1389 GCGC G^CGC
1831 GCGC
Ksp632I 677 CTCTTC CTCTTC(1/4)
1164 CTCTTC
MamI 76 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1912 GATGCAGATC
MslI 633 CACGCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
823 CATGCCGGTG
NcoI 32 CCATGG C^CATGG
369 CCATGG
NlaIV 210 GGACCC GG[ACGT]^[ACGT]CC
1376 GGCTCC
SfcI 102 CTACAG C^T[GA][CT]AG
1679 CTGCAG
SphI 112 GCATGC GCATG^C
1085 GCATGC
StyI 32 CCATGG C^C[AT][AT]GG
369 CCATGG
TaqI 687 TCGA T^CGA
1217 TCGA
TfiI 1684 GATTC G^A[AT]TC
1762 GATTC
Tsp45I 610 GTCAC ^GT[GC]AC
782 GTCAC
TthHB8I 687 TCGA T^CGA
1217 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbvI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraIII DsaI EcoRII FauI Fnu4HI
FokI HapII HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
Tru9I TseI Tsp509I TspRI XhoII