(Circular) (Six-base) MAPSORT of: pFN21AB3754.seq Check: 1815 from: 1 to: 6754 pFN21AB3754 6754 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2896 2896 Size: 6754 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3960 4817 6201 6469 1632 Size: 2328 857 1384 268 1917 Fragments arranged by size: 2328 1917 1384 857 268 AflII C'TTAA_G Cuts at: 820 1017 3661 820 Size: 197 2644 3913 Fragments arranged by size: 3913 2644 197 AflIII A'CryG_T Cuts at: 1203 2235 2871 5653 6630 1203 Size: 1032 636 2782 977 1327 Fragments arranged by size: 2782 1327 1032 977 636 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3213 3267 3861 1184 Size: 2029 54 594 4077 Fragments arranged by size: 4077 2029 594 54 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3366 3398 3366 Size: 32 6722 AlwNI CAG_nnn'CTG Cuts at: 2592 2668 4018 4497 6069 2592 Size: 76 1350 479 1572 3277 Fragments arranged by size: 3277 1572 1350 479 76 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 2382 3730 3773 4063 4289 4441 6550 1493 Size: 889 1348 43 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 1348 889 290 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 4079 1870 Size: 73 2136 4545 Fragments arranged by size: 4545 2136 73 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4075 5530 618 Size: 325 217 627 2288 1455 1842 Fragments arranged by size: 2288 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2077 3990 4073 1391 Size: 686 1913 83 4072 Fragments arranged by size: 4072 1913 686 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2056 2707 928 Size: 971 157 651 4975 Fragments arranged by size: 4975 971 651 157 BbvCI CC'TCA_GC Cuts at: 3186 3186 Size: 6754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4945 4979 1903 Size: 34 3008 34 3678 Fragments arranged by size: 3678 3008 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BfrBI ATG'CAT Cuts at: 2909 2909 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5365 136 Size: 107 122 71 631 605 3693 1525 Fragments arranged by size: 3693 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2080 6749 2080 Size: 4669 2085 BlpI GC'TnA_GC Cuts at: 3255 4171 3255 Size: 916 5838 Bme1580I G_kGCm'C Cuts at: 1790 4815 5971 1790 Size: 3025 1156 2573 Fragments arranged by size: 3025 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2874 1318 Size: 1556 5198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4485 5443 442 Size: 416 53 83 399 24 3068 958 1753 Fragments arranged by size: 3068 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3216 3216 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2461 2839 3413 3974 3998 5414 1239 Size: 1222 378 574 561 24 1416 2579 Fragments arranged by size: 2579 1416 1222 574 561 378 24 Bpu10I CC'TnA_GC Cuts at: 2126 2924 3186 2126 Size: 798 262 5694 Fragments arranged by size: 5694 798 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 3116 4879 5744 6042 2061 Size: 1055 1763 865 298 2773 Fragments arranged by size: 2773 1763 1055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3473 5417 882 Size: 617 1974 1944 2219 Fragments arranged by size: 2219 1974 1944 617 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 2085 3921 6748 2085 Size: 1836 2827 2091 Fragments arranged by size: 2827 2091 1836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4561 4943 5578 275 Size: 53 83 186 936 3028 382 635 1451 Fragments arranged by size: 3028 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2365 2722 3555 5187 5585 5859 6006 6417 2365 Size: 357 833 1632 398 274 147 411 2702 Fragments arranged by size: 2702 1632 833 411 398 357 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3366 3396 1178 Size: 30 165 30 1963 30 4536 Fragments arranged by size: 4536 1963 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 3052 3374 3502 1186 Size: 1138 728 322 128 4438 Fragments arranged by size: 4438 1138 728 322 128 BseYI C'CCAG_C Cuts at: 3518 5957 6453 3518 Size: 2439 496 3819 Fragments arranged by size: 3819 2439 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3953 1163 Size: 573 2217 3964 Fragments arranged by size: 3964 2217 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 3202 3990 4815 4900 5971 2259 Size: 943 788 825 85 1071 3042 Fragments arranged by size: 3042 1071 943 825 788 85 BsmI GAATG_Cn' Cuts at: 4265 4358 4265 Size: 93 6661 BsmBI CGTCTCn'nnnn_ Cuts at: 3180 3180 Size: 6754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2259 3202 3990 4073 4815 4900 Size: 399 287 182 943 788 83 742 85 Cuts at: 4900 5971 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 943 788 742 399 287 182 85 83 BspEI T'CCGG_A Cuts at: 2365 6417 2365 Size: 4052 2702 BspHI T'CATG_A Cuts at: 3471 4643 6373 3471 Size: 1172 1730 3852 Fragments arranged by size: 3852 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2896 4109 844 Size: 2052 1213 3489 Fragments arranged by size: 3489 2052 1213 BsrBI CCG'CTC Cuts at: 1394 1823 3510 4641 1394 Size: 429 1687 1131 3507 Fragments arranged by size: 3507 1687 1131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2186 5249 5423 65 Size: 1183 938 3063 174 1396 Fragments arranged by size: 3063 1396 1183 938 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2831 3108 5398 5585 1700 Size: 110 1021 277 2290 187 2869 Fragments arranged by size: 2869 2290 1021 277 187 110 BsrGI T'GTAC_A Cuts at: 96 3625 96 Size: 3529 3225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 4814 5826 4814 Size: 1012 5742 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstXI CCAn_nnnn'nTGG Cuts at: 3367 3367 Size: 6754 BstYI r'GATC_y Cuts at: 1972 2080 2588 2650 3291 3796 3922 4084 4451 Size: 108 508 62 641 505 126 162 367 Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 641 508 505 385 367 346 162 126 108 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 3064 3064 Size: 6754 BtgI C'CryG_G Cuts at: 513 1065 2037 2374 2538 513 Size: 552 972 337 164 4729 Fragments arranged by size: 4729 972 552 337 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 2669 4270 5064 5084 2669 Size: 1601 794 20 4339 Fragments arranged by size: 4339 1601 794 20 ClaI AT'CG_AT Cuts at: 1554 3222 1554 Size: 1668 5086 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2973 3008 529 Size: 35 2409 35 4275 Fragments arranged by size: 4275 2409 35 35 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DraIII CAC_nnn'GTG Cuts at: 2161 2421 3004 2161 Size: 260 583 5911 Fragments arranged by size: 5911 583 260 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719 Size: 54 1425 50 275 18 2371 889 1629 Cuts at: 6719 8 Size: 43 Fragments arranged by size: 2371 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 3175 4684 1353 Size: 1335 487 1509 3423 Fragments arranged by size: 3423 1509 1335 487 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3988 3988 Size: 6754 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2461 2839 3413 3960 3974 3998 4817 Size: 393 829 378 574 547 14 24 819 Cuts at: 4817 5414 6201 6469 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 829 819 787 597 574 547 393 378 268 24 14 EcoO109I rG'GnC_Cy Cuts at: 2555 2555 Size: 6754 EcoRI G'AATT_C Cuts at: 3730 4063 4441 3730 Size: 333 378 6043 Fragments arranged by size: 6043 378 333 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 5901 1678 Size: 4223 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2059 2091 2418 2450 2858 Size: 32 135 32 487 32 327 32 408 Cuts at: 2858 2890 5395 5427 5469 5501 1373 Size: 32 2505 32 42 32 2626 Fragments arranged by size: 2626 2505 487 408 327 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4098 4344 669 Size: 988 281 2160 246 3079 Fragments arranged by size: 3079 2160 988 281 246 HindIII A'AGCT_T Cuts at: 748 2123 3321 4029 748 Size: 1375 1198 708 3473 Fragments arranged by size: 3473 1375 1198 708 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2119 3441 5868 6052 1305 Size: 814 1322 2427 184 2007 Fragments arranged by size: 2427 2007 1322 814 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4932 Fragments arranged by size: 4932 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2642 2832 4712 5071 5230 518 Size: 648 1476 190 1880 359 159 2042 Fragments arranged by size: 2042 1880 1476 648 359 190 159 MspA1I CmG'CkG Cuts at: 1606 2056 3247 3976 4170 4847 5995 6240 1606 Size: 450 1191 729 194 677 1148 245 2120 Fragments arranged by size: 2120 1191 1148 729 677 450 245 194 NcoI C'CATG_G Cuts at: 513 1065 2037 2374 513 Size: 552 972 337 4893 Fragments arranged by size: 4893 972 552 337 NdeI CA'TA_TG Cuts at: 387 2168 387 Size: 1781 4973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NsiI A_TGCA'T Cuts at: 2911 2911 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 2121 2831 3094 4112 5657 1132 Size: 97 892 710 263 1018 1545 2229 Fragments arranged by size: 2229 1545 1018 892 710 263 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6754 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6754 PfoI T'CCnGG_A Cuts at: 3429 3429 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3366 3398 4823 4855 6336 6368 3366 Size: 32 1425 32 1481 32 3752 Fragments arranged by size: 3752 1481 1425 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2555 2555 Size: 6754 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PstI C_TGCA'G Cuts at: 830 3688 4106 830 Size: 2858 418 3478 Fragments arranged by size: 3478 2858 418 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2056 3247 3976 2056 Size: 1191 729 4834 Fragments arranged by size: 4834 1191 729 SacI G_AGCT'C Cuts at: 3990 3990 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SexAI A'CCwGG_T Cuts at: 2157 2157 Size: 6754 SfcI C'TryA_G Cuts at: 826 1045 2107 3684 4102 5237 5918 6109 826 Size: 219 1062 1577 418 1135 681 191 1471 Fragments arranged by size: 1577 1471 1135 1062 681 418 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 3095 3661 4858 5759 6021 Size: 197 926 133 1019 566 1197 901 262 Cuts at: 6021 820 Size: 1553 Fragments arranged by size: 1553 1197 1019 926 901 566 262 197 133 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 2121 3094 4112 1132 Size: 989 973 1018 3774 Fragments arranged by size: 3774 1018 989 973 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 2037 2374 4193 513 Size: 552 972 337 1819 3074 Fragments arranged by size: 3074 1819 972 552 337 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4781 4798 4951 5136 5581 325 Size: 1255 3201 17 153 185 445 1498 Fragments arranged by size: 3201 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2868 3625 5000 Size: 274 80 33 51 1443 891 757 1375 Cuts at: 5000 6712 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1375 891 757 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6714 6746 6714 Size: 32 6722 Tth111I GACn'n_nGTC Cuts at: 1316 3965 1316 Size: 2649 4105 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2485 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 4883 4883 Size: 6754 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI