Restriction map for
pFN21AB3754
(Linear) (Six-base) MAPSORT of: KIBB3754.seq Check: 2637 from: 1 to: 2049
KIBB3754.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 891 2049
Size: 891 1158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1955 2049
Size: 1955 94
AflII C'TTAA_G
Cuts at: 0 1656 2049
Size: 1656 393
AflIII A'CryG_T
Cuts at: 0 230 866 2049
Size: 230 636 1183
Fragments arranged by size:
1183 636 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1208 1262 1856 2049
Size: 1208 54 594 193
Fragments arranged by size:
1208 594 193 54
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1361 1393 2049
Size: 1361 32 656
Fragments arranged by size:
1361 656 32
AlwNI CAG_nnn'CTG
Cuts at: 0 587 663 2013 2049
Size: 587 76 1350 36
Fragments arranged by size:
1350 587 76 36
ApoI r'AATT_y
Cuts at: 0 377 1725 1768 2049
Size: 377 1348 43 281
Fragments arranged by size:
1348 377 281 43
BanII G_rGCy'C
Cuts at: 0 72 1985 2049
Size: 72 1913 64
Fragments arranged by size:
1913 72 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 51 702 2049
Size: 51 651 1347
Fragments arranged by size:
1347 651 51
BbvCI CC'TCA_GC
Cuts at: 0 1181 2049
Size: 1181 868
BfrBI ATG'CAT
Cuts at: 0 904 2049
Size: 904 1145
BglII A'GATC_T
Cuts at: 0 75 2049
Size: 75 1974
BlpI GC'TnA_GC
Cuts at: 0 1250 2049
Size: 1250 799
BmgBI CAC'GTC
Cuts at: 0 869 2049
Size: 869 1180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1211 2049
Size: 1211 838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 834 1408 1969 1993 2049
Size: 456 378 574 561 24 56
Fragments arranged by size:
574 561 456 378 56 24
Bpu10I CC'TnA_GC
Cuts at: 0 121 919 1181 2049
Size: 121 798 262 868
Fragments arranged by size:
868 798 262 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1111 2049
Size: 56 1055 938
Fragments arranged by size:
1055 938 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 1468 2049
Size: 1468 581
BsaBI GATnn'nnATC
Cuts at: 0 80 1916 2049
Size: 80 1836 133
Fragments arranged by size:
1836 133 80
BsaWI w'CCGG_w
Cuts at: 0 360 717 1550 2049
Size: 360 357 833 499
Fragments arranged by size:
833 499 360 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1361 1391 2049
Size: 1361 30 658
Fragments arranged by size:
1361 658 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 1047 1369 1497 2049
Size: 319 728 322 128 552
Fragments arranged by size:
728 552 322 319 128
BseYI C'CCAG_C
Cuts at: 0 1513 2049
Size: 1513 536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1948 2049
Size: 1948 101
BsiHKAI G_wGCw'C
Cuts at: 0 254 1197 1985 2049
Size: 254 943 788 64
Fragments arranged by size:
943 788 254 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1175 2049
Size: 1175 874
Bsp1286I G_dGCh'C
Cuts at: 0 72 254 1197 1985 2049
Size: 72 182 943 788 64
Fragments arranged by size:
943 788 182 72 64
BspEI T'CCGG_A
Cuts at: 0 360 2049
Size: 360 1689
BspHI T'CATG_A
Cuts at: 0 1466 2049
Size: 1466 583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 891 2049
Size: 891 1158
BsrBI CCG'CTC
Cuts at: 0 1505 2049
Size: 1505 544
BsrDI GCAATG_nn'
Cuts at: 0 181 2049
Size: 181 1868
BsrFI r'CCGG_y
Cuts at: 0 826 1103 2049
Size: 826 277 946
Fragments arranged by size:
946 826 277
BsrGI T'GTAC_A
Cuts at: 0 1620 2049
Size: 1620 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 2049
Size: 1362 687
BstYI r'GATC_y
Cuts at: 0 75 583 645 1286 1791 1917 2049
Size: 75 508 62 641 505 126 132
Fragments arranged by size:
641 508 505 132 126 75 62
Bsu36I CC'TnA_GG
Cuts at: 0 1059 2049
Size: 1059 990
BtgI C'CryG_G
Cuts at: 0 32 369 533 2049
Size: 32 337 164 1516
Fragments arranged by size:
1516 337 164 32
BtsI GCAGTG_nn'
Cuts at: 0 664 2049
Size: 664 1385
ClaI AT'CG_AT
Cuts at: 0 1217 2049
Size: 1217 832
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 968 1003 2049
Size: 968 35 1046
Fragments arranged by size:
1046 968 35
DraIII CAC_nnn'GTG
Cuts at: 0 156 416 999 2049
Size: 156 260 583 1050
Fragments arranged by size:
1050 583 260 156
EarI CTCTTCn'nnn_
Cuts at: 0 683 1170 2049
Size: 683 487 879
Fragments arranged by size:
879 683 487
EcoICRI GAG'CTC
Cuts at: 0 1983 2049
Size: 1983 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 834 1408 1955 1969 1993 2049
Size: 456 378 574 547 14 24 56
Fragments arranged by size:
574 547 456 378 56 24 14
EcoO109I rG'GnC_Cy
Cuts at: 0 550 2049
Size: 550 1499
EcoRI G'AATT_C
Cuts at: 0 1725 2049
Size: 1725 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 413 445 853 885 2049
Size: 54 32 327 32 408 32 1164
Fragments arranged by size:
1164 408 327 54 32 32 32
HindIII A'AGCT_T
Cuts at: 0 118 1316 2024 2049
Size: 118 1198 708 25
Fragments arranged by size:
1198 708 118 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1436 2049
Size: 114 1322 613
Fragments arranged by size:
1322 613 114
MslI CAynn'nnrTG
Cuts at: 0 637 827 2049
Size: 637 190 1222
Fragments arranged by size:
1222 637 190
MspA1I CmG'CkG
Cuts at: 0 51 1242 1971 2049
Size: 51 1191 729 78
Fragments arranged by size:
1191 729 78 51
NcoI C'CATG_G
Cuts at: 0 32 369 2049
Size: 32 337 1680
Fragments arranged by size:
1680 337 32
NdeI CA'TA_TG
Cuts at: 0 163 2049
Size: 163 1886
NsiI A_TGCA'T
Cuts at: 0 906 2049
Size: 906 1143
NspI r_CATG'y
Cuts at: 0 116 826 1089 2049
Size: 116 710 263 960
Fragments arranged by size:
960 710 263 116
PfoI T'CCnGG_A
Cuts at: 0 1424 2049
Size: 1424 625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1361 1393 2049
Size: 1361 32 656
Fragments arranged by size:
1361 656 32
PpuMI rG'GwC_Cy
Cuts at: 0 550 2049
Size: 550 1499
PstI C_TGCA'G
Cuts at: 0 1683 2049
Size: 1683 366
PvuII CAG'CTG
Cuts at: 0 51 1242 1971 2049
Size: 51 1191 729 78
Fragments arranged by size:
1191 729 78 51
SacI G_AGCT'C
Cuts at: 0 1985 2049
Size: 1985 64
SexAI A'CCwGG_T
Cuts at: 0 152 2049
Size: 152 1897
SfcI C'TryA_G
Cuts at: 0 102 1679 2049
Size: 102 1577 370
Fragments arranged by size:
1577 370 102
SmlI C'TyrA_G
Cuts at: 0 71 1090 1656 2049
Size: 71 1019 566 393
Fragments arranged by size:
1019 566 393 71
SphI G_CATG'C
Cuts at: 0 116 1089 2049
Size: 116 973 960
Fragments arranged by size:
973 960 116
StyI C'CwwG_G
Cuts at: 0 32 369 2049
Size: 32 337 1680
Fragments arranged by size:
1680 337 32
TatI w'GTAC_w
Cuts at: 0 863 1620 2049
Size: 863 757 429
Fragments arranged by size:
863 757 429
Tth111I GACn'n_nGTC
Cuts at: 0 1960 2049
Size: 1960 89
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 2049
Size: 480 1569
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AloI AlwNI ApoI
BanII BbsI BbvCI BfrBI BglII BlpI BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraIII EarI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HindIII MmeI MslI MspA1I NcoI NdeI
NsiI NspI PfoI PpiI PpuMI PstI PvuII SacI
SexAI SfcI SmlI SphI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BglI Bme1580I
BmrI BmtI BsaAI BsaHI BsiEI BsiWI BsmI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI DraI DrdI
EaeI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI ZraI