(Circular) (Six-base) MAPSORT of: pFN21AB3747.seq Check: 979 from: 1 to: 5683 pFN21AB3747 5683 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2376 4582 5559 1203 Size: 1173 2206 977 1327 Fragments arranged by size: 2206 1327 1173 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 2473 4412 2473 Size: 1939 3744 AleI CACnn'nnGTG Cuts at: 1166 2819 1166 Size: 1653 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 2619 Size: 32 5651 AlwNI CAG_nnn'CTG Cuts at: 3426 4998 3426 Size: 1572 4111 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2065 2992 3218 3370 5479 1493 Size: 572 927 226 152 2109 1697 Fragments arranged by size: 2109 1697 927 572 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2296 3008 1870 Size: 73 353 712 4545 Fragments arranged by size: 4545 712 353 73 BamHI G'GATC_C Cuts at: 2778 3013 3380 2778 Size: 235 367 5081 Fragments arranged by size: 5081 367 235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2807 3004 4459 618 Size: 325 217 627 1020 197 1455 1842 Fragments arranged by size: 1842 1455 1020 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 2303 2343 2538 2697 3002 1391 Size: 912 40 195 159 305 4072 Fragments arranged by size: 4072 912 305 195 159 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2043 2379 928 Size: 971 129 15 336 4232 Fragments arranged by size: 4232 971 336 129 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2574 2608 3874 3908 1903 Size: 34 637 34 1266 34 3678 Fragments arranged by size: 3678 1266 637 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 4921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 2922 4294 Size: 107 122 71 631 605 1231 19 1372 Cuts at: 4294 136 Size: 1525 Fragments arranged by size: 1525 1372 1231 631 605 122 107 71 19 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 3744 4900 1790 Size: 1954 1156 2573 Fragments arranged by size: 2573 1954 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 4343 1239 Size: 1007 2097 2579 Fragments arranged by size: 2579 2097 1007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2275 3808 4673 4971 2275 Size: 1533 865 298 2987 Fragments arranged by size: 2987 1533 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2209 3474 493 Size: 711 1005 1265 2702 Fragments arranged by size: 2702 1265 1005 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 2676 2703 3490 Size: 53 83 186 936 786 357 27 787 Cuts at: 3490 3872 4507 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 787 786 635 382 357 186 83 53 27 BsaWI w'CCGG_w Cuts at: 2460 4116 4514 4788 4935 5346 2460 Size: 1656 398 274 147 411 2797 Fragments arranged by size: 2797 1656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 2711 2741 1178 Size: 30 165 30 1216 30 62 30 4120 Fragments arranged by size: 4120 1216 165 62 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 2716 1186 Size: 1393 137 4153 Fragments arranged by size: 4153 1393 137 BseYI C'CCAG_C Cuts at: 4886 5382 4886 Size: 496 5187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2477 3133 3894 4922 1540 Size: 275 186 476 656 761 1028 2301 Fragments arranged by size: 2301 1028 761 656 476 275 186 BsiHKAI G_wGCw'C Cuts at: 2303 3744 3829 4900 2303 Size: 1441 85 1071 3086 Fragments arranged by size: 3086 1441 1071 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2303 2343 2538 2697 3002 3744 3829 Size: 399 513 40 195 159 305 742 85 Cuts at: 3829 4900 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 305 195 159 85 40 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 844 Size: 2194 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2691 3570 1394 Size: 429 868 879 3507 Fragments arranged by size: 3507 879 868 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 2328 96 Size: 2232 3451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 2417 3743 4755 2417 Size: 1326 1012 3345 Fragments arranged by size: 3345 1326 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2778 3013 3380 3765 3782 5234 5320 5332 Size: 806 235 367 385 17 1452 86 12 Cuts at: 5332 5678 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 806 385 367 346 235 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2345 2670 513 Size: 552 1280 325 3526 Fragments arranged by size: 3526 1280 552 325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2915 487 Size: 683 56 1689 3255 Fragments arranged by size: 3255 1689 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2904 3130 Size: 54 1425 50 275 18 201 873 226 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 873 275 226 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2481 3613 1353 Size: 1128 1132 3423 Fragments arranged by size: 3423 1132 1128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2410 4270 4644 4790 387 Size: 1064 138 821 1860 374 146 1280 Fragments arranged by size: 1860 1280 1064 821 374 146 138 EcoICRI GAG'CTC Cuts at: 2301 2301 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 3746 4343 5130 5398 1239 Size: 393 614 1500 597 787 268 1524 Fragments arranged by size: 1524 1500 787 614 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2759 2759 Size: 5683 EcoO109I rG'GnC_Cy Cuts at: 2413 2630 2413 Size: 217 5466 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2459 2491 4324 Size: 32 135 32 794 32 61 32 1833 Cuts at: 4324 4356 4398 4430 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1833 794 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 2707 3027 3273 669 Size: 988 281 139 630 320 246 3079 Fragments arranged by size: 3079 988 630 320 281 246 139 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 4797 4981 1305 Size: 1152 2340 184 2007 Fragments arranged by size: 2340 2007 1152 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 2787 Fragments arranged by size: 2787 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2543 2819 3641 4000 4159 518 Size: 648 1113 264 276 822 359 159 2042 Fragments arranged by size: 2042 1113 822 648 359 276 264 159 MspA1I CmG'CkG Cuts at: 1606 2337 2347 2599 3099 3776 4924 5169 1606 Size: 731 10 252 500 677 1148 245 2120 Fragments arranged by size: 2120 1148 731 677 500 252 245 10 NcoI C'CATG_G Cuts at: 513 1065 2670 513 Size: 552 1605 3526 Fragments arranged by size: 3526 1605 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2380 3041 4586 1132 Size: 97 1151 661 1545 2229 Fragments arranged by size: 2229 1545 1151 661 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 2376 4582 2376 Size: 2206 3477 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2619 2651 3752 3784 5265 5297 2619 Size: 32 1101 32 1481 32 3005 Fragments arranged by size: 3005 1481 1101 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2413 2413 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2045 3035 830 Size: 1215 990 3478 Fragments arranged by size: 3478 1215 990 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2599 2599 Size: 5683 SacI G_AGCT'C Cuts at: 2303 2303 Size: 5683 SacII CC_GC'GG Cuts at: 2348 2348 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2041 2430 3031 4166 4847 5038 826 Size: 219 996 389 601 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 681 601 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2254 3787 4688 4950 820 Size: 197 926 311 1533 901 262 1553 Fragments arranged by size: 1553 1533 926 901 311 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 2670 3122 513 Size: 552 1605 452 3074 Fragments arranged by size: 3074 1605 552 452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 3727 3880 4065 4510 325 Size: 1255 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 3929 5641 Size: 274 80 33 51 1443 351 1601 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1601 1443 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI