(Circular) (Six-base) MAPSORT of: pFN21AB3652.seq Check: 6025 from: 1 to: 7009 pFN21AB3652 7009 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4819 278 Size: 53 83 186 936 3283 2468 Fragments arranged by size: 3283 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 4352 1937 Size: 382 2033 4594 Fragments arranged by size: 4594 2033 382 Acc65I G'GTAC_C Cuts at: 4330 4330 Size: 7009 AclI AA'CG_TT Cuts at: 1563 5136 5509 1563 Size: 3573 373 3063 Fragments arranged by size: 3573 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3876 4029 4185 4301 5072 6456 6724 1632 Size: 2244 153 156 116 771 1384 268 1917 Fragments arranged by size: 2244 1917 1384 771 268 156 153 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6812 AflIII A'CryG_T Cuts at: 1203 5908 6885 1203 Size: 4705 977 1327 Fragments arranged by size: 4705 1327 977 AgeI A'CCGG_T Cuts at: 5840 5840 Size: 7009 AhdI GACnn_n'nnGTC Cuts at: 5738 5738 Size: 7009 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7009 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2214 1184 Size: 1030 5979 AlwNI CAG_nnn'CTG Cuts at: 2741 4752 6324 2741 Size: 2011 1572 3426 Fragments arranged by size: 3426 2011 1572 ApaI G_GGCC'C Cuts at: 3779 3779 Size: 7009 ApaLI G'TGCA_C Cuts at: 2717 5066 6222 2717 Size: 2349 1156 3504 Fragments arranged by size: 3504 2349 1156 ApoI r'AATT_y Cuts at: 1493 3131 3625 4318 4544 4696 6805 1493 Size: 1638 494 693 226 152 2109 1697 Fragments arranged by size: 2109 1697 1638 693 494 226 152 AseI AT'TA_AT Cuts at: 160 1032 5563 160 Size: 872 4531 1606 Fragments arranged by size: 4531 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7009 AvaI C'yCGr_G Cuts at: 1870 1943 4133 4334 1870 Size: 73 2190 201 4545 Fragments arranged by size: 4545 2190 201 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2292 2325 2292 Size: 33 6976 BamHI G'GATC_C Cuts at: 4339 4706 4339 Size: 367 6642 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 4330 5785 618 Size: 325 217 627 341 2202 1455 1842 Fragments arranged by size: 2202 1842 1455 627 341 325 217 BanII G_rGCy'C Cuts at: 1391 2916 3779 3854 4328 1391 Size: 1525 863 75 474 4072 Fragments arranged by size: 4072 1525 863 474 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3341 3416 3963 928 Size: 971 1442 75 547 3974 Fragments arranged by size: 3974 1442 971 547 75 BbvCI CC'TCA_GC Cuts at: 4180 4180 Size: 7009 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5200 5234 1903 Size: 34 3263 34 3678 Fragments arranged by size: 3678 3263 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4901 6111 4901 Size: 1210 5799 BclI T'GATC_A Cuts at: 1548 1548 Size: 7009 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3725 4160 5620 Size: 107 122 71 631 605 2053 435 1460 Cuts at: 5620 136 Size: 1525 Fragments arranged by size: 2053 1525 1460 631 605 435 122 107 71 BglII A'GATC_T Cuts at: 2473 7004 2473 Size: 4531 2478 BlpI GC'TnA_GC Cuts at: 4426 4426 Size: 7009 Bme1580I G_kGCm'C Cuts at: 1790 2721 3290 3779 5070 6226 1790 Size: 931 569 489 1291 1156 2573 Fragments arranged by size: 2573 1291 1156 931 569 489 BmgBI CAC'GTC Cuts at: 1318 2704 3757 1318 Size: 1386 1053 4570 Fragments arranged by size: 4570 1386 1053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4740 5698 442 Size: 416 53 83 399 24 3323 958 1753 Fragments arranged by size: 3323 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7009 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3833 3885 3971 4002 4212 5669 1239 Size: 2594 52 86 31 210 1457 2579 Fragments arranged by size: 2594 2579 1457 210 86 52 31 Bpu10I CC'TnA_GC Cuts at: 4180 4180 Size: 7009 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2131 5134 5999 6297 2131 Size: 3003 865 298 2843 Fragments arranged by size: 3003 2843 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2493 2752 3081 4230 5672 882 Size: 617 994 259 329 1149 1442 2219 Fragments arranged by size: 2219 1442 1149 994 617 329 259 BsaAI yAC'GTr Cuts at: 493 1204 3445 4800 493 Size: 711 2241 1355 2702 Fragments arranged by size: 2702 2241 1355 711 BsaBI GATnn'nnATC Cuts at: 7003 7003 Size: 7009 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4816 5198 5833 275 Size: 53 83 186 936 3283 382 635 1451 Fragments arranged by size: 3283 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 3079 3473 5442 5840 6114 6261 6672 2901 Size: 178 394 1969 398 274 147 411 3238 Fragments arranged by size: 3238 1969 411 398 394 274 178 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2607 2637 2919 2949 3696 Size: 30 165 30 1204 30 282 30 747 Cuts at: 3696 3726 1178 Size: 30 4461 Fragments arranged by size: 4461 1204 747 282 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3830 4094 4205 1186 Size: 1768 876 264 111 3990 Fragments arranged by size: 3990 1768 876 264 111 BseYI C'CCAG_C Cuts at: 2323 2731 3932 4150 6212 6708 2323 Size: 408 1201 218 2062 496 2624 Fragments arranged by size: 2624 2062 1201 496 408 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2676 3027 1163 Size: 573 940 351 5145 Fragments arranged by size: 5145 940 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2083 4459 5220 6248 1540 Size: 275 186 82 2376 761 1028 2301 Fragments arranged by size: 2376 2301 1028 761 275 186 82 BsiHKAI G_wGCw'C Cuts at: 2721 3770 5070 5155 6226 2721 Size: 1049 1300 85 1071 3504 Fragments arranged by size: 3504 1300 1071 1049 85 BsmI GAATG_Cn' Cuts at: 2829 4520 4613 2829 Size: 1691 93 5225 Fragments arranged by size: 5225 1691 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2721 2916 3290 3770 3779 3854 4328 Size: 399 931 195 374 480 9 75 474 Cuts at: 4328 5070 5155 6226 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 931 742 480 474 399 374 195 85 75 9 BspEI T'CCGG_A Cuts at: 2901 6672 2901 Size: 3771 3238 BspHI T'CATG_A Cuts at: 2815 4898 6628 2815 Size: 2083 1730 3196 Fragments arranged by size: 3196 2083 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4364 844 Size: 3520 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4896 1394 Size: 429 3073 3507 Fragments arranged by size: 3507 3073 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2760 5504 5678 65 Size: 1183 1512 2744 174 1396 Fragments arranged by size: 2744 1512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2660 5653 5840 1700 Size: 110 850 2993 187 2869 Fragments arranged by size: 2993 2869 850 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7009 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7009 BssSI C'ACGA_G Cuts at: 2019 2915 5069 6081 2019 Size: 896 2154 1012 2947 Fragments arranged by size: 2947 2154 1012 896 BstAPI GCAn_nnn'nTGC Cuts at: 794 2687 2741 794 Size: 1893 54 5062 Fragments arranged by size: 5062 1893 54 BstBI TT'CG_AA Cuts at: 2992 5813 2992 Size: 2821 4188 BstEII G'GTnAC_C Cuts at: 1184 2032 1184 Size: 848 6161 BstXI CCAn_nnnn'nTGG Cuts at: 3949 3949 Size: 7009 BstYI r'GATC_y Cuts at: 1972 2399 2473 2982 3612 4339 4706 5091 5108 Size: 427 74 509 630 727 367 385 17 Cuts at: 5108 6560 6646 6658 7004 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 727 630 509 427 385 367 346 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2824 2908 3771 4203 513 Size: 552 1759 84 863 432 3319 Fragments arranged by size: 3319 1759 863 552 432 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3243 4233 487 Size: 683 56 2017 990 3263 Fragments arranged by size: 3263 2017 990 683 56 BtsI GCAGTG_nn' Cuts at: 4525 5319 5339 4525 Size: 794 20 6195 Fragments arranged by size: 6195 794 20 ClaI AT'CG_AT Cuts at: 1554 3190 1554 Size: 1636 5373 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6974 DraI TTT'AAA Cuts at: 4313 4313 Size: 7009 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3026 6016 809 Size: 507 1710 2990 1802 Fragments arranged by size: 2990 1802 1710 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3000 3355 4456 Size: 54 1425 50 275 18 1170 355 1101 Cuts at: 4456 5345 6974 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1170 1101 889 355 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4456 1537 Size: 275 2644 4090 Fragments arranged by size: 4090 2644 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2838 3852 4161 4280 4292 4939 1353 Size: 812 673 1014 309 119 12 647 3423 Fragments arranged by size: 3423 1014 812 673 647 309 119 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5596 5970 6116 387 Size: 1064 138 4007 374 146 1280 Fragments arranged by size: 4007 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3833 3876 3885 3971 4002 4029 4185 Size: 393 2201 43 9 86 31 27 156 Cuts at: 4185 4212 4301 5072 5669 6456 6724 1239 Size: 27 89 771 597 787 268 1524 Fragments arranged by size: 2201 1524 787 771 597 393 268 156 89 86 43 31 27 27 9 EcoNI CCTnn'n_nnAGG Cuts at: 2141 3698 2141 Size: 1557 5452 EcoO109I rG'GnC_Cy Cuts at: 2876 3776 4114 2876 Size: 900 338 5771 Fragments arranged by size: 5771 900 338 EcoRI G'AATT_C Cuts at: 4318 4696 4318 Size: 378 6631 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2217 2217 Size: 7009 FspI TGC'GCA Cuts at: 5515 5515 Size: 7009 HaeII r_GCGC'y Cuts at: 1678 2792 6156 1678 Size: 1114 3364 2531 Fragments arranged by size: 3364 2531 1114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5650 5682 5724 5756 1373 Size: 32 135 32 4078 32 42 32 2626 Fragments arranged by size: 4078 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4353 4599 669 Size: 988 281 2415 246 3079 Fragments arranged by size: 3079 2415 988 281 246 HindIII A'AGCT_T Cuts at: 748 3369 748 Size: 2621 4388 HpaI GTT'AAC Cuts at: 4599 4599 Size: 7009 KpnI G_GTAC'C Cuts at: 4334 4334 Size: 7009 MfeI C'AATT_G Cuts at: 4608 4608 Size: 7009 MluI A'CGCG_T Cuts at: 6885 6885 Size: 7009 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2905 6123 6307 1305 Size: 1600 3218 184 2007 Fragments arranged by size: 3218 2007 1600 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3002 10 Size: 54 1425 343 1170 4017 Fragments arranged by size: 4017 1425 1170 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3149 4967 5326 5485 518 Size: 648 1983 1818 359 159 2042 Fragments arranged by size: 2042 1983 1818 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3328 4184 4205 4425 5102 6250 6495 1606 Size: 1722 856 21 220 677 1148 245 2120 Fragments arranged by size: 2120 1722 1148 856 677 245 220 21 NaeI GCC'GGC Cuts at: 2662 2662 Size: 7009 NcoI C'CATG_G Cuts at: 513 1065 2824 2908 513 Size: 552 1759 84 4614 Fragments arranged by size: 4614 1759 552 84 NdeI CA'TA_TG Cuts at: 387 387 Size: 7009 NgoMIV G'CCGG_C Cuts at: 2660 2660 Size: 7009 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7009 NotI GC'GGCC_GC Cuts at: 4456 4456 Size: 7009 NspI r_CATG'y Cuts at: 1132 1229 4367 5912 1132 Size: 97 3138 1545 2229 Fragments arranged by size: 3138 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3071 1348 Size: 1723 5286 PciI A'CATG_T Cuts at: 5908 5908 Size: 7009 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7009 PfoI T'CCnGG_A Cuts at: 3594 3594 Size: 7009 PmeI GTTT'AAAC Cuts at: 4313 4313 Size: 7009 PmlI CAC'GTG Cuts at: 3445 3445 Size: 7009 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2529 2561 5078 5110 6591 6623 2529 Size: 32 2517 32 1481 32 2915 Fragments arranged by size: 2915 2517 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7009 PsiI TTA'TAA Cuts at: 4579 4579 Size: 7009 PspOMI G'GGCC_C Cuts at: 3775 3775 Size: 7009 PstI C_TGCA'G Cuts at: 830 3462 4361 830 Size: 2632 899 3478 Fragments arranged by size: 3478 2632 899 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7009 PvuII CAG'CTG Cuts at: 4184 4184 Size: 7009 SacII CC_GC'GG Cuts at: 4206 4206 Size: 7009 SalI G'TCGA_C Cuts at: 1936 4351 1936 Size: 2415 4594 SapI GCTCTTCn'nnn_ Cuts at: 4280 4280 Size: 7009 SbfI CC_TGCA'GG Cuts at: 4361 4361 Size: 7009 ScaI AGT'ACT Cuts at: 2108 5257 6969 2108 Size: 3149 1712 2148 Fragments arranged by size: 3149 2148 1712 SfcI C'TryA_G Cuts at: 826 1045 3169 3458 4357 5492 6173 6364 826 Size: 219 2124 289 899 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 899 681 289 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3725 3725 Size: 7009 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7009 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7009 SmaI CCC'GGG Cuts at: 1872 4336 1872 Size: 2464 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2110 5113 6014 6276 820 Size: 197 926 167 3003 901 262 1553 Fragments arranged by size: 3003 1553 926 901 262 197 167 SnaBI TAC'GTA Cuts at: 493 4800 493 Size: 4307 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7009 SphI G_CATG'C Cuts at: 1132 4367 1132 Size: 3235 3774 SspI AAT'ATT Cuts at: 5 52 4933 5 Size: 47 4881 2081 Fragments arranged by size: 4881 2081 47 StuI AGG'CCT Cuts at: 1517 2265 2945 1517 Size: 748 680 5581 Fragments arranged by size: 5581 748 680 StyI C'CwwG_G Cuts at: 513 1065 2797 2824 2908 3723 4448 513 Size: 552 1732 27 84 815 725 3074 Fragments arranged by size: 3074 1732 815 725 552 84 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 2472 5036 5053 5206 5391 5836 Size: 1255 517 375 2564 17 153 185 445 Cuts at: 5836 325 Size: 1498 Fragments arranged by size: 2564 1498 1255 517 445 375 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 3048 5255 Size: 274 80 33 51 1443 129 942 2207 Cuts at: 5255 6967 96 Size: 1712 138 Fragments arranged by size: 2207 1712 1443 942 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2725 3696 3728 4044 4076 6969 7001 2693 Size: 32 971 32 316 32 2893 32 2701 Fragments arranged by size: 2893 2701 971 316 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7009 XbaI T'CTAG_A Cuts at: 4345 4345 Size: 7009 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 3668 2266 Size: 1402 5607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7009 XmaI C'CCGG_G Cuts at: 1870 4334 1870 Size: 2464 4545 XmnI GAAnn'nnTTC Cuts at: 4259 5138 4259 Size: 879 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4817 276 Size: 53 83 186 936 3283 2468 Fragments arranged by size: 3283 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI