(Circular) (Six-base) MAPSORT of: pFN21AB3511.seq Check: 5599 from: 1 to: 6106 pFN21AB3511 6106 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2895 3916 278 Size: 53 83 186 936 1359 1021 2468 Fragments arranged by size: 2468 1359 1021 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3332 4169 5553 5821 1632 Size: 1700 837 1384 268 1917 Fragments arranged by size: 1917 1700 1384 837 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3398 1184 Size: 2214 3892 AlwNI CAG_nnn'CTG Cuts at: 2396 2840 2869 3849 5421 2396 Size: 444 29 980 1572 3081 Fragments arranged by size: 3081 1572 980 444 29 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2279 2828 3415 3641 3793 5902 1493 Size: 786 549 587 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 587 549 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 3431 1870 Size: 73 1488 4545 Fragments arranged by size: 4545 1488 73 BamHI G'GATC_C Cuts at: 2384 3436 3803 2384 Size: 1052 367 4687 Fragments arranged by size: 4687 1052 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2311 2524 2617 3427 Size: 325 217 627 513 11 213 93 810 Cuts at: 3427 4882 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 810 627 513 325 217 213 93 11 BanII G_rGCy'C Cuts at: 1391 2082 2369 2799 3425 1391 Size: 691 287 430 626 4072 Fragments arranged by size: 4072 691 626 430 287 BbeI G_GCGC'C Cuts at: 2528 2528 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BbvCI CC'TCA_GC Cuts at: 2355 2355 Size: 6106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3053 3087 4297 4331 1903 Size: 34 1116 34 1210 34 3678 Fragments arranged by size: 3678 1210 1116 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3114 3270 6101 3114 Size: 156 2831 3119 Fragments arranged by size: 3119 2831 156 BlpI GC'TnA_GC Cuts at: 3306 3523 3306 Size: 217 5889 Bme1580I G_kGCm'C Cuts at: 1790 2316 2989 4167 5323 1790 Size: 526 673 1178 1156 2573 Fragments arranged by size: 2573 1178 1156 673 526 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 3837 4795 Size: 416 53 83 399 24 901 1519 958 Cuts at: 4795 442 Size: 1753 Fragments arranged by size: 1753 1519 958 901 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2757 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 2628 2837 2936 4766 1239 Size: 1379 10 209 99 1830 2579 Fragments arranged by size: 2579 1830 1379 209 99 10 Bpu10I CC'TnA_GC Cuts at: 2355 2841 3059 2355 Size: 486 218 5402 Fragments arranged by size: 5402 486 218 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4231 5096 5394 4231 Size: 865 298 4943 Fragments arranged by size: 4943 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2525 2892 3913 4295 Size: 53 83 186 936 992 367 1021 382 Cuts at: 4295 4930 275 Size: 635 1451 Fragments arranged by size: 1451 1021 992 936 635 382 367 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 1178 Size: 30 165 30 1158 30 4693 Fragments arranged by size: 4693 1158 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2807 1186 Size: 1621 4485 BseYI C'CCAG_C Cuts at: 2230 2247 3371 5309 5805 2230 Size: 17 1124 1938 496 2531 Fragments arranged by size: 2531 1938 1124 496 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 2799 4167 4252 5323 2263 Size: 536 1368 85 1071 3046 Fragments arranged by size: 3046 1368 1071 536 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2263 2316 2369 2799 2989 3425 Size: 399 292 181 53 53 430 190 436 Cuts at: 3425 4167 4252 5323 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 430 399 292 190 181 85 53 53 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2812 3461 844 Size: 1968 649 3489 Fragments arranged by size: 3489 1968 649 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4601 4775 65 Size: 1183 1821 1532 174 1396 Fragments arranged by size: 1821 1532 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 3358 794 Size: 2564 3542 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 2238 2652 2238 Size: 414 5692 BstYI r'GATC_y Cuts at: 1972 2384 3114 3270 3436 3803 4188 4205 5657 Size: 412 730 156 166 367 385 17 1452 Cuts at: 5657 5743 5755 6101 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 730 412 385 367 346 166 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2742 2742 Size: 6106 BtgI C'CryG_G Cuts at: 513 1065 2004 2520 2701 513 Size: 552 939 516 181 3918 Fragments arranged by size: 3918 939 552 516 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2554 2580 3262 3622 4416 4436 2554 Size: 26 682 360 794 20 4224 Fragments arranged by size: 4224 794 682 360 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3140 3553 4442 Size: 54 1425 50 275 18 1310 413 889 Cuts at: 4442 6071 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1310 889 413 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2762 4036 1353 Size: 798 611 1274 3423 Fragments arranged by size: 3423 1274 798 611 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3251 4693 5067 5213 387 Size: 1064 138 1662 1442 374 146 1280 Fragments arranged by size: 1662 1442 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2797 2797 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2628 2837 2936 3332 4169 4766 Size: 393 986 10 209 99 396 837 597 Cuts at: 4766 5553 5821 1239 Size: 787 268 1524 Fragments arranged by size: 1524 986 837 787 597 396 393 268 209 99 10 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 6106 EcoO109I rG'GnC_Cy Cuts at: 2767 2774 2837 2767 Size: 7 63 6036 Fragments arranged by size: 6036 63 7 EcoRI G'AATT_C Cuts at: 2279 3415 3793 2279 Size: 1136 378 4592 Fragments arranged by size: 4592 1136 378 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2528 5253 1678 Size: 850 2725 2531 Fragments arranged by size: 2725 2531 850 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2647 2679 2895 2927 4747 Size: 32 135 32 1075 32 216 32 1820 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1820 1075 216 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2524 2524 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 2542 3705 2542 Size: 1163 4943 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3142 10 Size: 54 1425 343 1310 2974 Fragments arranged by size: 2974 1425 1310 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2729 4064 4423 4582 518 Size: 648 1563 1335 359 159 2042 Fragments arranged by size: 2042 1563 1335 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2925 3310 3319 3522 4199 5347 5592 Size: 645 674 385 9 203 677 1148 245 Cuts at: 5592 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 674 645 385 245 203 9 NarI GG'CG_CC Cuts at: 2525 2525 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 2520 513 Size: 552 939 516 4099 Fragments arranged by size: 4099 939 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2132 3379 3464 5009 1132 Size: 97 903 1247 85 1545 2229 Fragments arranged by size: 2229 1545 1247 903 97 85 PasI CC'CwG_GG Cuts at: 1348 2075 2362 2403 1348 Size: 727 287 41 5051 Fragments arranged by size: 5051 727 287 41 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PfoI T'CCnGG_A Cuts at: 2481 2913 2481 Size: 432 5674 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2767 2774 2837 2767 Size: 7 63 6036 Fragments arranged by size: 6036 63 7 PshAI GACnn'nnGTC Cuts at: 1613 2772 1613 Size: 1159 4947 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 2564 3270 3458 830 Size: 1734 706 188 3478 Fragments arranged by size: 3478 1734 706 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2251 3310 3319 2251 Size: 1059 9 5038 Fragments arranged by size: 5038 1059 9 SacI G_AGCT'C Cuts at: 2799 2799 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SanDI GG'GwC_CC Cuts at: 2774 2774 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2187 2560 2635 3266 3454 4589 5270 Size: 219 1142 373 75 631 188 1135 681 Cuts at: 5270 5461 826 Size: 191 1471 Fragments arranged by size: 1471 1142 1135 681 631 373 219 191 188 75 SfoI GGC'GCC Cuts at: 2526 2526 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4210 5111 5373 820 Size: 197 926 2267 901 262 1553 Fragments arranged by size: 2267 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3379 3464 1132 Size: 2247 85 3774 Fragments arranged by size: 3774 2247 85 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 2180 1517 Size: 663 5443 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 2520 3545 513 Size: 552 939 300 216 1025 3074 Fragments arranged by size: 3074 1025 939 552 300 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2967 4352 6064 Size: 274 80 33 51 1443 990 1385 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1712 1443 1385 990 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2561 2593 6066 6098 2561 Size: 32 3473 32 2569 Fragments arranged by size: 3473 2569 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2060 2060 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 2870 4235 2870 Size: 1365 4741 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2893 3914 276 Size: 53 83 186 936 1359 1021 2468 Fragments arranged by size: 2468 1359 1021 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI