Restriction map for
pFN21AB3483
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Van91I XbaI XcmI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 622 CGCG CG^CG
AluI 351 AGCT AG^CT
Alw44I 526 GTGCAC G^TGCAC
AlwNI 347 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 151 CCTCAGG CC^T[ACGT]AGG
ApaLI 526 GTGCAC G^TGCAC
AspHI 526 GTGCAC G[AT]GC[AT]^C
AspI 532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 114 CCCGGG C^[CT]CG[GA]G
BalI 38 TGGCCA TGG^CCA
BanI 280 GGCACC G^G[CT][GA]CC
BmyI 526 GTGCAC G[AGT]GC[ACT]^C
BsaBI 636 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 598 GAGACC GGTCTC(1/5)
BseRI 705 CTCCTC GAGGAG(10/8)
BsiHKAI 526 GTGCAC G[AT]GC[AT]^C
BsmAI 598 GAGAC GTCTC(1/5)
BsmFI 243 GGGAC GGGAC(10/14)
BsoBI 114 CCCGGG C^[CT]CG[GA]G
Bsp1286I 526 GTGCAC G[AGT]GC[ACT]^C
BstEII 564 GGTCACC G^GT[ACGT]ACC
BstPI 564 GGTCACC G^GT[ACGT]ACC
BstUI 622 CGCG CG^CG
BstXI 228 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 151 CCTCAGG CC^T[ACGT]AGG
Cac8I 404 GCAGGC GC[ACGT]^[ACGT]GC
DraII 368 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 38 TGGCCA [CT]^GGCC[GA]
EarI 700 CTCTTC CTCTTC(1/4)
Eco81I 151 CCTCAGG CC^T[ACGT]AGG
EcoO109I 368 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 564 GGTCACC G^GT[ACGT]ACC
HaeII 71 AGCGCC [GA]GCGC^[CT]
HapII 115 CCGG C^CGG
HgaI 536 GCGTC GACGC(5/10)
HinfI 661 GAATC G^A[ACGT]TC
HpaII 115 CCGG C^CGG
Ksp632I 700 CTCTTC CTCTTC(1/4)
MaeII 605 ACGT A^CGT
MamI 636 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
MseI 608 TTAA T^TAA
MspA1I 350 CAGCTG C[AC]G^C[GT]G
MspI 115 CCGG C^CGG
MvnI 622 CGCG CG^CG
PflFI 532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 344 CTGCAG CTGCA^G
PvuII 350 CAGCTG CAG^CTG
SfaNI 21 GATGC GCATC(5/9)
SmaI 114 CCCGGG CCC^GGG
StuI 668 AGGCCT AGG^CCT
TaqI 688 TCGA T^CGA
TfiI 661 GAATC G^A[AT]TC
Tru9I 608 TTAA T^TAA
Tth111I 532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 688 TCGA T^CGA
XmaCI 114 CCCGGG C^CCGGG
XmaI 114 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 720 GATCC GGATC(4/5)
685 GGATC
AvaII 438 GGACC G^G[AT]CC
696 GGTCC
BcnI 114 CCCGG CC^[GC]GG
115 CCGGG
BglI 128 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
371 GCCTTGGCGGC
BpmI 83 CTGGAG CTGGAG(16/14)
131 CTGGAG
BsrI 97 ACTGG ACTGG(1/-1)
648 CCAGT
CfoI 72 GCGC GCG^C
621 GCGC
DdeI 152 CTCAG C^T[ACGT]AG
665 CTCAG
DsaI 228 CCATGG C^C[GA][CT]GG
494 CCATGG
HhaI 72 GCGC GCG^C
621 GCGC
HinP1I 72 GCGC G^CGC
621 GCGC
MboII 701 TCTTC GAAGA(8/7)
743 TCTTC
NciI 114 CCCGG CC^[GC]GG
115 CCGGG
NcoI 228 CCATGG C^CATGG
494 CCATGG
NlaIV 280 GGCACC GG[ACGT]^[ACGT]CC
368 GGGGCC
NspI 383 ACATGC [GA]CATG^[CT]
458 ACATGC
SfcI 344 CTGCAG C^T[GA][CT]AG
486 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboI
MnlI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI