(Circular) (Six-base) MAPSORT of: pFN21AB3483.seq Check: 8375 from: 1 to: 5464 pFN21AB3483 5464 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 4911 5179 1632 Size: 1895 1384 268 1917 Fragments arranged by size: 1917 1895 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 2357 3207 4779 2357 Size: 850 1572 3042 Fragments arranged by size: 3042 1572 850 ApaLI G'TGCA_C Cuts at: 2531 3521 4677 2531 Size: 990 1156 3318 Fragments arranged by size: 3318 1156 990 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2789 1870 Size: 73 176 670 4545 Fragments arranged by size: 4545 670 176 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2285 2785 4240 618 Size: 325 217 627 498 500 1455 1842 Fragments arranged by size: 1842 1455 627 500 498 325 217 BanII G_rGCy'C Cuts at: 1391 2783 1391 Size: 1392 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 2382 4075 Size: 107 122 71 631 605 467 243 1693 Cuts at: 4075 136 Size: 1525 Fragments arranged by size: 1693 1525 631 605 467 243 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2535 3525 4681 1790 Size: 745 990 1156 2573 Fragments arranged by size: 2573 1156 990 745 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2112 2647 3195 Size: 416 53 83 399 24 695 535 548 Cuts at: 3195 4153 442 Size: 958 1753 Fragments arranged by size: 1753 958 695 548 535 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2685 2685 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2157 4124 1239 Size: 870 48 1967 2579 Fragments arranged by size: 2579 1967 870 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 4127 882 Size: 617 1098 1530 2219 Fragments arranged by size: 2219 1530 1098 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 2645 5458 2645 Size: 2813 2651 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2701 1186 Size: 1515 3949 BseYI C'CCAG_C Cuts at: 2172 2196 2220 2244 2268 2357 2392 4667 5163 Size: 24 24 24 24 89 35 2275 496 Cuts at: 5163 2172 Size: 2473 Fragments arranged by size: 2473 2275 496 89 35 24 24 24 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4891 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 3525 3610 4681 2535 Size: 990 85 1071 3318 Fragments arranged by size: 3318 1071 990 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 2783 3525 3610 4681 1391 Size: 399 745 248 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 399 248 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2819 844 Size: 1975 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3959 4133 65 Size: 1183 2711 174 1396 Fragments arranged by size: 2711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 2569 1184 Size: 1385 4079 BstXI CCAn_nnnn'nTGG Cuts at: 2240 2240 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459 Size: 822 367 385 17 1452 86 12 346 Cuts at: 5459 1972 Size: 1977 Fragments arranged by size: 1977 1452 822 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2157 2157 Size: 5464 BtgI C'CryG_G Cuts at: 513 1065 2004 2233 2499 513 Size: 552 939 229 266 3478 Fragments arranged by size: 3478 939 552 266 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2370 2437 2980 3774 3794 2370 Size: 67 543 794 20 4040 Fragments arranged by size: 4040 794 543 67 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2911 3800 Size: 54 1425 50 275 18 213 868 889 Cuts at: 3800 5429 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 868 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2711 3394 1353 Size: 1358 683 3423 Fragments arranged by size: 3423 1358 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2157 3527 4124 4911 5179 1239 Size: 393 477 48 1370 597 787 268 1524 Fragments arranged by size: 1524 1370 787 597 477 393 268 48 EcoO109I rG'GnC_Cy Cuts at: 2374 2374 Size: 5464 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2080 4611 1678 Size: 402 2531 2531 Fragments arranged by size: 2531 2531 402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 4105 4137 4179 Size: 32 135 32 1079 32 1422 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 1422 1079 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 3429 Fragments arranged by size: 3429 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 2214 2238 2262 3422 3781 3940 Size: 648 1024 24 24 24 1160 359 159 Cuts at: 3940 518 Size: 2042 Fragments arranged by size: 2042 1160 1024 648 359 159 24 24 24 MspA1I CmG'CkG Cuts at: 1606 2357 2880 3557 4705 4950 1606 Size: 751 523 677 1148 245 2120 Fragments arranged by size: 2120 1148 751 677 523 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2233 2499 513 Size: 552 939 229 266 3478 Fragments arranged by size: 3478 939 552 266 229 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2392 2467 2822 4367 1132 Size: 97 1163 75 355 1545 2229 Fragments arranged by size: 2229 1545 1163 355 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PstI C_TGCA'G Cuts at: 830 2353 2816 830 Size: 1523 463 3478 Fragments arranged by size: 3478 1523 463 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2357 2357 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2349 2491 2812 3947 4628 4819 826 Size: 219 1304 142 321 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 681 321 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2121 2791 1872 Size: 249 670 4545 Fragments arranged by size: 4545 670 249 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2675 1517 Size: 1158 4306 StyI C'CwwG_G Cuts at: 513 1065 2004 2014 2233 2377 2499 2583 2903 Size: 552 939 10 219 144 122 84 320 Cuts at: 2903 513 Size: 3074 Fragments arranged by size: 3074 939 552 320 219 144 122 84 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 2621 2666 3491 3508 3661 3846 Size: 1255 723 318 45 825 17 153 185 Cuts at: 3846 4291 325 Size: 445 1498 Fragments arranged by size: 1498 1255 825 723 445 318 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 2508 3710 Size: 274 80 33 51 1443 510 21 1202 Cuts at: 3710 5422 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1202 510 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2709 2741 5424 5456 2709 Size: 32 2683 32 2717 Fragments arranged by size: 2717 2683 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2540 1316 Size: 1224 4240 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2119 2789 1870 Size: 249 670 4545 Fragments arranged by size: 4545 670 249 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI