Restriction map for
pFN21AB3483
(Linear) (Six-base) MAPSORT of: KIBB3483.seq Check: 1311 from: 1 to: 759
KIBB3483.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 352 759
Size: 352 407
ApaLI G'TGCA_C
Cuts at: 0 526 759
Size: 526 233
AvaI C'yCGr_G
Cuts at: 0 114 759
Size: 114 645
BanI G'GyrC_C
Cuts at: 0 280 759
Size: 280 479
BglI GCCn_nnn'nGGC
Cuts at: 0 134 377 759
Size: 134 243 382
Fragments arranged by size:
382 243 134
Bme1580I G_kGCm'C
Cuts at: 0 530 759
Size: 530 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 107 642 759
Size: 107 535 117
Fragments arranged by size:
535 117 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 680 759
Size: 680 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 152 759
Size: 104 48 607
Fragments arranged by size:
607 104 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 759
Size: 592 167
BsaBI GATnn'nnATC
Cuts at: 0 640 759
Size: 640 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 759
Size: 696 63
BseYI C'CCAG_C
Cuts at: 0 167 191 215 239 263 352 387 759
Size: 167 24 24 24 24 89 35 372
Fragments arranged by size:
372 167 89 35 24 24 24 24
BsiHKAI G_wGCw'C
Cuts at: 0 530 759
Size: 530 229
Bsp1286I G_dGCh'C
Cuts at: 0 530 759
Size: 530 229
BstEII G'GTnAC_C
Cuts at: 0 564 759
Size: 564 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 235 759
Size: 235 524
Bsu36I CC'TnA_GG
Cuts at: 0 152 759
Size: 152 607
BtgI C'CryG_G
Cuts at: 0 228 494 759
Size: 228 266 265
Fragments arranged by size:
266 265 228
BtsI GCAGTG_nn'
Cuts at: 0 365 432 759
Size: 365 67 327
Fragments arranged by size:
365 327 67
EaeI y'GGCC_r
Cuts at: 0 38 759
Size: 38 721
EarI CTCTTCn'nnn_
Cuts at: 0 706 759
Size: 706 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 152 759
Size: 104 48 607
Fragments arranged by size:
607 104 48
EcoO109I rG'GnC_Cy
Cuts at: 0 369 759
Size: 369 390
HaeII r_GCGC'y
Cuts at: 0 75 759
Size: 75 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 759
Size: 646 32 81
Fragments arranged by size:
646 81 32
MscI TGG'CCA
Cuts at: 0 40 759
Size: 40 719
MslI CAynn'nnrTG
Cuts at: 0 185 209 233 257 759
Size: 185 24 24 24 502
Fragments arranged by size:
502 185 24 24 24
MspA1I CmG'CkG
Cuts at: 0 352 759
Size: 352 407
NcoI C'CATG_G
Cuts at: 0 228 494 759
Size: 228 266 265
Fragments arranged by size:
266 265 228
NspI r_CATG'y
Cuts at: 0 387 462 759
Size: 387 75 297
Fragments arranged by size:
387 297 75
PstI C_TGCA'G
Cuts at: 0 348 759
Size: 348 411
PvuII CAG'CTG
Cuts at: 0 352 759
Size: 352 407
SfcI C'TryA_G
Cuts at: 0 344 486 759
Size: 344 142 273
Fragments arranged by size:
344 273 142
SmaI CCC'GGG
Cuts at: 0 116 759
Size: 116 643
StuI AGG'CCT
Cuts at: 0 670 759
Size: 670 89
StyI C'CwwG_G
Cuts at: 0 9 228 372 494 578 759
Size: 9 219 144 122 84 181
Fragments arranged by size:
219 181 144 122 84 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 616 661 759
Size: 298 318 45 98
Fragments arranged by size:
318 298 98 45
TatI w'GTAC_w
Cuts at: 0 482 503 759
Size: 482 21 256
Fragments arranged by size:
482 256 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 704 736 759
Size: 704 32 23
Fragments arranged by size:
704 32 23
Tth111I GACn'n_nGTC
Cuts at: 0 535 759
Size: 535 224
XmaI C'CCGG_G
Cuts at: 0 114 759
Size: 114 645
Enzymes that do cut:
AlwNI ApaLI AvaI BanI BglI Bme1580I BmrI BplI
BpmI BsaI BsaBI BseRI BseYI BsiHKAI Bsp1286I BstEII
BstXI Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoO109I
HaeII Hin4I MscI MslI MspA1I NcoI NspI PstI
PvuII SfcI SmaI StuI StyI TaqII TatI TstI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI
XmnI ZraI