Restriction map for
pFN21AB3451
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BbvI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BmyI BpmI BsaAI
BsaHI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeIII Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MluNI
MscI MseI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tru9I TseI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 491 TCCGGA T^CCGGA
AcsI 247 AAATTC [GA]^AATT[CT]
ApoI 247 AAATTC [GA]^AATT[CT]
Asp700I 265 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 518 CCCGAG C^[CT]CG[GA]G
BcgI 732 CGAGACAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 466 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 466 GCTCAGC GC^T[ACGT]AGC
BsaBI 504 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 181 GGTCTC GGTCTC(1/5)
BsaWI 491 TCCGGA [AT]^CCGG[AT]
BseAI 491 TCCGGA T^CCGGA
BsgI 526 GTGCAG GTGCAG(16/14)
BsiYI 300 CCATGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 300 CCATGGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 728 GGGAC GGGAC(10/14)
BsoBI 518 CCCGAG C^[CT]CG[GA]G
BspEI 491 TCCGGA T^CCGGA
BspHI 512 TCATGA T^CATGA
BspMI 105 ACCTGC ACCTGC(4/8)
BssKI 149 CCAGG ^CC[ACGT]GG
BstNI 149 CCAGG CC^[AT]GG
BstYI 309 GGATCT [GA]^GATC[CT]
Cac8I 598 GCTTGC GC[ACGT]^[ACGT]GC
CelII 466 GCTCAGC GC^T[ACGT]AGC
CfoI 21 GCGC GCG^C
DraII 151 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 151 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 149 CCAGG ^CC[AT]GG
Fnu4HI 35 GCGGC GC^[ACGT]GC
HaeII 20 AGCGCC [GA]GCGC^[CT]
HapII 492 CCGG C^CGG
HgaI 138 GACGC GACGC(5/10)
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HpaII 492 CCGG C^CGG
ItaI 35 GCGGC GC^[ACGT]GC
MaeII 371 ACGT A^CGT
MamI 504 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 309 GGATCT [GA]^GATC[CT]
MroI 491 TCCGGA T^CCGGA
MslI 370 CACGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 492 CCGG C^CGG
MvaI 149 CCAGG CC^[AT]GG
PflMI 300 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 207 GAGTC GAGTC(4/5)
PpuMI 151 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 138 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 512 TCATGA T^CATGA
ScrFI 149 CCAGG CC^[ACGT]GG
SfaNI 172 GCATC GCATC(5/9)
SmlI 184 CTCAAG C^T[CT][GA]AG
SspI 127 AATATT AAT^ATT
TspRI 723 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 300 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 309 GGATCT [GA]^GATC[CT]
XmnI 265 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 67 GATCC GGATC(4/5)
309 GGATC
AvaII 152 GGACC G^G[AT]CC
480 GGTCC
BbsI 453 GAAGAC GAAGAC(2/6)
628 GTCTTC
BpuAI 453 GAAGAC GAAGAC(2/6)
628 GTCTTC
BsmAI 733 GAGAC GTCTC(1/5)
182 GTCTC
BsrI 724 ACTGG ACTGG(1/-1)
775 CCAGT
Cfr13I 152 GGACC G^G[ACGT]CC
480 GGTCC
DdeI 77 CTAAG C^T[ACGT]AG
467 CTCAG
DpnII 67 GATC ^GATC
310 GATC
DsaI 300 CCATGG C^C[GA][CT]GG
335 CCATGG
HphI 697 GGTGA GGTGA(8/7)
146 TCACC
MboI 67 GATC ^GATC
310 GATC
MwoI 651 GCTGAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
681 GCTCATGGAGC
NcoI 300 CCATGG C^CATGG
335 CCATGG
NdeII 67 GATC ^GATC
310 GATC
Sau3AI 67 GATC ^GATC
310 GATC
Sau96I 152 GGACC G^G[ACGT]CC
480 GGTCC
TfiI 564 GATTC G^A[AT]TC
784 GAATC
Tsp45I 145 GTCAC ^GT[GC]AC
320 GTGAC
XcmI 50 CCAAGAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
662 CCAAGTTTGAATTGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI AluI BsaJI EcoT14I HinfI
MaeIII MboII MnlI NlaIII NlaIV
StyI Tsp509I